Stem Cells; a Potential Treatment of Autoimmune Diseases
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Date
2022
Journal Title
Journal ISSN
Volume Title
Type
Other
Publisher
October university for modern sciences and arts
Series Info
Biochemistry Graduation Project 2020 - 2022;
Doi
Scientific Journal Rankings
Abstract
Rheumatoid arthritis is an auto-immune disease characterized by loss of self-tolerance in the joints;
consequently resulting in inflammation in joints, cartilage destruction, and bone erosion (Mitra,
2013). Recent studies deducted that stem cells are a promising candidate that could achieve a
balanced equilibrium between addressing the pathophysiology of RA and overcoming conventional
drugs` disadvantages. MSCs are multipotent stem cells primarily transformed in the bone marrow; in
addition, it has a crucial role in repairing and forming skeletal tissues, such as; adipocytes,
chondrocytes, and osteocytes (Greish et al., 2012). Besides MSCs' chondrogenic differentiation
potential, it also orchestrates the activity of the immune system through direct and indirect
interactions and it also has a significant anti-inflammatory effect. Also, the plant extract of Paeonia
lactiflora Pallas that shows a great anti-inflammatory effect in different studies is going to be tested
alone and in combination with MSC to observe if it potentiates its effect. On the other hand, the
objective involves a brief discussion of the pathophysiology of rheumatoid arthritis, stem cells, and
their isolation scheme as well as the method of disease induction. Moreover, it also involves the
investigation of disease parameters and exploring the modulatory effect of mesenchymal stem cells
on RA. At the end of the treatment period real-time PCR was used to detect the following genes:
alkaline phosphatase (mALP), transcription factor sp7 (mOsterix) and collagen type I or alpha 1
(mCOL-I). Moreover, a western blot was also employed to measure both Sirt-1 and Foxp3 in the
joints. The results showed a significant increase in osteogenic markers and a significant reduction in
pro-inflammatory cytokines, in the MSC group and the plant extract group when compared to the
other groups (P<0.05). To sum up, this study illustrated that MSC and plant extract showed a
beneficial role in the treatment of RA through their anti-inflammatory and immunosuppressive
properties. Moreover, it had a much more significant role when both were combined as it provided a
better prognosis than when MSC is used alone
Description
Keywords
October University For Modern Sciences and Arts, MSA, October University For Modern Sciences and Arts MSA, جامعة اكتوبر للعلوم الحديثة والاداب, Rheumatoid Arthritis, Stem Cells, Anti-rheumatic Drugs
Citation
Faculty Of Pharmacy Graduation Project 2020 - 2022