WOUND HEALING AND ANTI INFLAMMATORY EFFECTS OF PROPOLIS IN CRITICAL SIZE DEFECT OF RAT BUCCAL MUCOSA (HISTOCHEMICAL AND IMMUNO HISTOCHEMICAL STUDY)

Abstract

Objectives: the aim of this study is to evaluate the healing and anti-inflammatory effects of propolis in critical wound defects in rats ‘ buccal mucosa. Subjects& methods: 20 adult male albino rats of average weight 150-250 grams were used. Rats were divided into 2 groups Group I: consists of 10 rats, which were subdivided into 2 sub groups Sub Group I A: consists of 5 rats, the wounds were left un treated for 3 days Sub Group I B: consists of 5 rats, the wounds were left un treated for 7 days Group II: Consists of 10 rats which were subdivided into 2 sub groups Sub Group II A: consists of 5 rats, the wounds were treated with propolis for 3 days Sub Group II B: consists of 5 rats, the wounds were treated with propolis for 7 days The wounds were examined histologically, histochemically and immunohistochemically Results: Histologiacally, histochemical and immunohistochemical results showed that the healing process was faster in propolis treated rats due to anti inflammatory and anti oxidant effects of Propolis. Immunohistochemical labeling of vimentin in propolis treated groups was higher than un treated groups Conclusion: Propolis could improve wound healing by stimulating the proliferation of epithelial cells; and fibroblast proliferation, thus improving wound closure and decreasing inflammation (as prolonged inflammation and the release of inflammatory cytokines could cause tissue damage)