The Role of microRNAs in the pathogenesis of acute myocardial infarction (RSPB2.8)

Abstract

MicroRNAs (miRNAs) are considered as a small non-coding RNA which regulateexpression of gene by preventing the translation including destruction of specific mRNA.Acute myocardial infarction (AMI) is characterized by inflammation, cardiomyocyteapoptosis, and cardiac necrosis that may develop heart failure. Necroptosis is a form ofregulated necrosis and is dependent on a signaling pathway involving receptor interactingprotein kinase (RIPK). Fas-associated protein with death domain (FADD) is a negativeregulator for necroptosis. MicroRNAs play a critical role in the pathogenesis ofcardiovascular diseases. 10 mice were randomly assigned into two groups: normal controlgroup and induction group by s.c injection of isoprenaline (100 mg/kg). Heart injury wasevaluated through the histological examination in heart tissue, in addition to thebiochemical assessment of troponin I. The levels of miRNA-103 in heart tissues weredetermined using based miRNA quantitative real-time polymerase chain reactions (qRT-PCRs). The following parameters were investigated for studying the possiblemechanisms of miRNA-103 in necrosis: FADD, RIPK and IL-6 (Interleukine-6). Theresults revealed that in AMI group miRNA 103 was significantly elevated while theexpression level of FADD was decreased, moreover RIPK and IL-6 were significantlyincreased. In exploring the molecular mechanism of miRNA 103 in AMI, The presentstudy provides new evidence showing that miRNA 103 up regulation through targetingFADD, resulting loss of FADD leads to resistance to apoptosis and cells undergonecroptosis instead. In conclusion, miR-103 might be considered a novel potentialbiomarkers for AMI and its modulation provide a new approach for preventing AMI.