The Use of CASA to Monitor Effects of Red Laser on Post-frozenBuffalo Semen

Abstract

Live biological tissue reacts to irradiation effects from different light sources and wavelengths, sperm cells especially exhibit physiological changes when exposed to laser light irradiation. The motility characteristics of the buffalo bull (Bubalusbubalis), are considered to be the most important qualities necessary for a successful fertilization process. The buffalo’s population in Egypt have a major socioeconomic impact, where Egypt is one of the major buffalo population holding countries in the African continent with 5,231,162 heads; and in the world with 3.1% of the world's total population. Economically, the buffalo meat makes up 46% of the market consumption of red meat in 2016, which highly effects the Egyptian economy. Therefore, the current study focuses on the improvement of semen motility characteristics and fertility capabilities of post-frozen Egyptian buffalo semen, through the use of red laser irradiation. The study describes the potential of utilizing laser irradiation as a simple and inexpensive method to improve the in situ characteristics of semen motility and overall quality, for the purpose of enhancing the fertility capabilities of the semen samples to be used in the artificial insemination processes. Results showed that the red laser light irradiation were highly capable of exciting and enhancing the post-frozen buffalo semen motility characteristics, both over the long term and short term evaluations. Significant changes in the motility characteristics indicated that the red laser light irradiation at 625nm wavelength is an effective treatment method for enhancing the motility characteristics of the post-frozen buffalo semen.