Evaluation of antibacterial activity of chitosan-coated anisotropic silver Nanoparticles on different bacterial strains

Abstract

The present study explores the antibacterial activity of Chitosan NPs when it is coated with different concentrations of silver NPs. CS-AgNPs materials were synthesized by a simple chemical method. The synthesized CS–Ag Nanoparticles contains two different ratio of silver to be able to demonstrate the effect of different concentrations of silver on the antibacterial activity of the synthesized product. Silver Nanoparticles were synthesized by chemical reduction method as well. The CS–Ag Nanoparticles was characterized using Fourier transform infrared spectroscopy (FTIR) that was used to study the conformational changes in the functional groups of the polymers present in CS-AgNPs, in addition to Transmission electron microscope (TEM) for determining The morphology and size, also atomic force microscopy (AFM) to provide topographical chart for the synthesized product, as well as X-ray diffraction (XRD) to investigate The crystalline and phase structure of CS-AgNPs. FTIR spectroscopy has confirmed the presence of both silver and chitosan Nanoparticles as it was also observed. TEM results have revealed that CS-AgNPs has a well capped multi structures and well distribution of silver on the nanoparticle surface. AFM results have indicated that silver NPs is well dispersed and loaded into the chitosan sheet. XRD pattern of CS-AgNPs has shown an amorphous shape for the product that was conducted from the broadening of peaks. The results have clearly indicated the interaction between chitosan and metallic surface of silver NPs which has led to effective stabilization of silver NPs on the chitosan polymer. CS-AgNPs antibacterial activity was assessed by measuring the inhibition zone when it was tested against Bacillus subtitles, Bacillus lichniforms, E. coli, and Enterobacter ludwigii, and has showed highly potent antibacterial activity.