Antibacterial Effect of Pre-constructed 3D Bone Scaffolds before and after Modification with Propolis

Abstract

AIM: This study was to determine and compare the antibacterial activity of different scaffold materials before and after their modification with ethanolic extract of Egyptian propolis ethanolic extract of propolis (EEP). SETTINGS AND DESIGN: Preparation of the dry mass of propolis, preparation of EEP, preparation of the scaffolds, and antibacterial activity testing. MATERIALS AND METHODS: Four bacterial strains were used to determine the antibacterial activity of two different scaffold materials before and after their modification with EEP (15% and 25% by weight). RESULTS: Tricalcium phosphate + gelatin binder modified by 25% EEP exhibited the highest antibacterial activity against Escherichia coli. While, tricalcium phosphate + (alginate and cellulose nanowhiskers) binder modified by 25% EEP demonstrated the highest antibacterial activity Staphylococcus aureus, Streptococcus mutans, and Lactobacillus casei. CONCLUSIONS: It can be concluded that EEP had a significant effect on the antibacterial activity of both scaffold materials; the antibacterial activity was higher against Gram-positive bacteria.