Comparative genomic study of post natal blood samples from patients suffering from infertility using Array CGH

Abstract

Infertility is the inability to conceive offsprings after 12 month of frequent mating. It is caused by hormonal, physiological and environmental misbalancing. Moreover, Premature ovarian failure, endometriosis and polycystic ovarian syndrome causes female infertility which will be discussed in this study regarding genes associated with those diseases. Therefore, the enrolled project is about comparative genomic study of post natal blood samples of females suffering from infertility using Microarray CGH. Blood samples were collected from the patients by the host institution, then the samples were prepared in the lab using Thermo Scientific GeneJET Genomic DNA Purification Kit #K0722 and Agilent Oligonucleotide Array-Based CGH for Genomic DNA Analysis protocols. The interpretation of results were done using Agilent feature extraction and cytogenomics software. Patients‟ results were interpreted and analysed showing deletion and amplification in genes located on chromosomes associated with infertility. These genes could be used and targeted in further studies and researches in manufacturing drugs.