Biodesulfurization of refractory sulfur compounds in petro-diesel by a novel hydrocarbon tolerable strain Paenibacillus glucanolyticus HN4
Date
44105
Journal Title
Journal ISSN
Volume Title
Type
Article
Publisher
Springer
Series Info
Environmental Science and Pollution Research;
Doi
Scientific Journal Rankings
Abstract
One of the main precursors of air pollution and acid rains is the presence of the recalcitrant thiophenic compounds, for example
dibenzothiophene (DBT) and its derivatives in transportation fuels. In an attempt to achieve the worldwide regulations of ultra-
low sulfur transportation fuels without affecting its hydrocarbon skeleton, a biphasic medium containing 100 mg/L DBT
dissolved in n-hexadecane (1/4 oil/water v/v) used for enrichment and isolation of selective biodesulfurizing bacterium from
an oil-polluted sediment sample collected from Egyptian Red Sea shoreline. The isolated bacterium is facultative anaerobe,
motile, spore-former, and mesophile. It is genetically identified as Paenibacillus glucanolyticus strain HN4 (NCBI Gene Bank
Accession No. MT645230). HN4 desulfurized DBT as a model of the recalcitrant thiophenic compounds without affecting its
hydrocarbon skeleton via the 4S-pathway producing 2-hydroxybiphenyl (2-HBP) as a dead end product. HN4 substantiated to be
a hydrocarbon tolerant, biosurfactants(s) producer, and endorsed unique enzymatic system capable of desulfurizing broad range
of thiophenic compounds and expressed an efficient desulfurization activity against the recalcitrant alkylated DBTs. As far our
knowledge, it is the first reported BDS study using P. glucanolyticus. Statistical optimization based on One-Factor-At-A-Time
(OFAT) technique and response surface methodology (RSM) applied for elucidation of mathematical model correlations de-
scribing and optimizing the effect of different physicochemical parameters on batch biphasic BDS process. That illustrated an
approximate increase in BDS efficiency by 1.34 fold and recorded 94% sulfur removal in biphasic batch process at optimum
operation conditions of 120 h, 0.14 wt% S-content model oil (DBT dissolved in n-hexadecane), 33.5 °C, pH7 and 1/1 oil/water
phase ratio, and 147 rpm. Resting cells of HN4 in a biphasic reactor (1/1 v/v) decreased the sulfur content of a refractory
thiophenic model oil (thiophene, benzothiophene, DBT, and alkylated DBT dissolved in n-hexadecane) from 0.14 to 0.027
wt%, and petro-diesel from 0.2 to 0.04 wt%, within 120 h, keeping the calorific value of the treated fuel intact. Consequently, that
novel strain could be recommended as a promising candidate
for BDS as complementary to hydrodesulfurization process in
oil refinery.
Description
Keywords
Model and real feed oil, Biosurfactants producer, Oil tolerance, Two-phase system, Selective biocatalytic desulfurization, Dibenzothiophene