Faculty Of Biotechnology Graduation Project 2018 - 2019

Permanent URI for this collectionhttp://185.252.233.37:4000/handle/123456789/40

Browse

Browsing Faculty Of Biotechnology Graduation Project 2018 - 2019 by Subject "Antibacterial"

Filter results by typing the first few letters
Now showing 1 - 2 of 2
  • No Thumbnail Available
    Item
    Antioxidant, antimicrobial and anticancer effect of African rose
    (October University for Modern Sciences and Arts, 2019) Abdelrahman Kamel, Teba
    Origin of African rose (plum) was reported for the first time in china 470 B.C. these was the first written document about plum. African rose have shown to have lots of medical uses and also have the ability to prevent diseases related to food, also in promoting of health. Moreover, African rose have been considered as a therapeutic treatment for pathologies that are associated with inflammation and oxidative stress. It was reveal that African rose phenolic compounds have so many of biological activities such as anti-inflammatory, anticancer, antioxidant and antimicrobial activities. The recent study is to analysis in vitro antimicrobial, anticancer and antioxidant activities of phenols, tannins, flavonoids and alkaloids that were extracted from African rose. These extractions were used to extract phenolic compounds from African rose this is done by ethanolic extraction method. Then to determine the phytochemicals HPLC analysis was used, DPPH was used to evaluate antioxidant activity, to determine antimicrobial activity agar discs diffusion method was used. There were three different cancer cell lines that had been taken from human there are Liver cell line (HepG2), Brest cancer cell line (MCF-7) and colon cancer cell line (Caco-2) colon cancer these to determine cytotoxic activity beside using of neutral red (NR) assay.
  • No Thumbnail Available
    Item
    Hydrogel Nanocomposite for Antibacterial Applications
    (October University for Modern Sciences and Arts, 2019) Hossam Fakhry, Sahar
    The development of hydrogels in the past few years in order to obtain an enhanced material with improved properties have led to the emergence of nanocomposite hydrogels that have unique properties which allows it to be used in various different fields and applications such as drug delivery, adsorption soil containing, tissue engineering, wound dressing and especially antimicrobial applications. Nanocomposite hydrogel are versatile materials that can be used as an alternative to the conventional antimicrobial agents that pathogens can become resistant to as the crosslinking of the polymeric materials along with the nanoparticles enhances the mechanical properties and the antimicrobial activity of the hydrogel. Thus, this study was conducted in order to fabricate a novel super absorbent terpolymer nanocomposite hydrogel through using the free radical co-polymerization method based on the usage of 2-Acrylamido-2-methylpropane sulfonic acid (AMPS), acrylamide, acrylonitrile and acrylic acid monomers and iron oxide (Fe2O3) magnetic nanoparticle and using benzoyl peroxide as initiator and ethylene glycol di- methacrylate (EGDMA) as crosslinker to test it for antimicrobial activity. The structure of the synthesized composite was confirmed using FTIR spectroscopy and XRD apparatus. Furthermore, the surface morphology and the magnetic nano-particle distributions were determined by SEM instrument. In addition to the that the swelling capacity of hydrogel nanocomposite was measured using the swelling test and lastly the efficiency of the produced composite was evaluated as antimicrobial agent for Gram positive Bacillus subtilis & Gram-negative Escherichia coli bacteria and Candida albicans fungal strain.