Browsing by Author "Salama M.M."

Now showing 1 - 3 of 3

Bioactive glycoalkaloides isolated from Solanum melongena fruit peels with potential anticancer properties against hepatocellular carcinoma cells
(Nature Publishing Group, 2019) Fekry M.I.; Ezzat, Shahira M; Salama M.M.; Alshehri O.Y.; Al-Abd A.M.; Pharmacognosy Department; Faculty of Pharmacy; Cairo University; Cairo; Egypt; Biomedical Research Section; Nawah Scientific; Mokattam; Cairo; Egypt; Department of Pharmacognosy; Faculty of Pharmacy; October University for Modern Science and Arts (MSA); 6th October; Giza; 12566; Egypt; Pharmacognosy Department; Faculty of Pharmacy; The British University of Egypt; El-Sherouk city; Cairo; Cairo 11837; Egypt; Research Centre; King Faisal Specialist Hospital and Research Center; Jeddah; 21499; Saudi Arabia; Pharmacology Department; Medical Division; National Research Centre; Dokki; Giza; Egypt; Department of Pharmaceutical Sciences; College of Pharmacy; Gulf Medical University; Ajman; Ajman 4184; United Arab Emirates
Hepatocellular carcinoma (HCC) is progressively increasing tumor with lack of accurate prognosis and inadequate systemic treatment approaches. Solanum sp. (such as Solanum melongena) is a folk herb which is reported to possess anticancer properties. In a continuity for our interest in pursuing the anticancer activity of compounds isolated from the fruit peels of Solanum melongena, the HPLC profiling and ESI-MS assessment for the methanolic extract evidenced the presence of bioactive glycoalkaloids (solasonine, solasodine and solamargine). These glycoalkaloids were isolated, purified and proved to possess in vitro cytotoxicity against human liver cancer cell lines (Huh7 and HepG2). Herein, we investigated the potential mechanism of action of these compounds using DNA content flow-cytometry and apoptosis/necrosis differential anaylsis using annexin-V/FITC staining. Solasonine, solasodine and solamargine inducd significant antiproliferative effect against liver cancer cells (Huh7 and HepG2) which was attributed to cell cycle arrest at S-phase. Solamargine, solasodine and solasonine induced significant apoptosis in Huh7 cells. Only solamargine-induced cell cycle arrest, was reflected as apoptotic cell killing effect against HepG2 cells. In conclusion, glycoalkaloids derived from Solanum melongena and particularly, solamargine are promising antiproliferative agents with potential anticancer effects. � 2019, The Author(s).
In vivo anti-inflammatory activity and UPLC-MS/MS profiling of the peels and pulps of Cucumis melo var. cantalupensis and Cucumis melo var. reticulatus
(Elsevier Ireland Ltd, 2019) Ezzat, Shahira M; Raslan M.; Salama M.M.; Menze E.T.; El Hawary S.S.; Department of Pharmacognosy; Faculty of Pharmacy; Cairo University; Cairo; 11562; Egypt; Department of Pharmacognosy; Faculty of Pharmacy; October University for Modern Science and Arts (MSA); 6th October12566; Egypt; Biotechnology and Life Sciences Department; Faculty of Postgraduate Studies for Advanced Sciences; Beni-Suef University62511; Egypt; Department of Pharmacognosy; Faculty of Pharmacy; The British University in Egypt; El-Sherouk City; Egypt; Department of Pharmacology and Toxicology; Faculty of Pharmacy; Ain Shams University; Cairo; 11566; Egypt
Ethnopharmacological relevance: Cucumis melo var. cantalupensis and Cucumis melo var. reticulatus are the most famous varieties of netted muskmelon or cantaloupe in Egypt. Cantaloupe has a great reputation as an anti-inflammatory drug for hot inflammation of liver, cough, eczema, and kidney disorders such as ulcers in the urinary tract, and our objective was to confirm this use scientifically. Materials and methods: Inflammation was induced in adult male Sprague Dawley rats by subcutaneous injection of 0.05 ml of carrageenan (1% solution in saline) into the plantar surface of the right hind paw 30 min after oral pretreatment of the rats with 95% ethanolic extracts of Cucumis melo var. cantalupensis peels (CCP) and pulps (CCU) and Cucumis melo var. reticulatus peels (CRP) and pulps (CRU) at doses of 25 and 50 mg/kg. Indomethacin (10 mg/kg) was used as a standard drug. The effect of the tested samples was measured on the oedema volume, as well as PGE-2, TNF-? IL-6 and IL-1? levels. Metabolic profiling of the extracts was performed using UPLC-MS/MS analysis. Results: Pretreatment of rats with the ethanol extract of the pulps and peels of the two varieties at doses of 25 and 50 mg/kg significantly inhibited the carrageenan-induced increase in the oedema volume of the rat paws after 3 h, except for the low dose of the French cantaloupe pulp. CRP at 50 mg/kg caused the most significant reductions in both TNF-? (P < 0.05) and IL-1? (P < 0.001) levels, while CCP caused the most significant reductions in PGE-2 and IL-6 (P < 0.05) levels. Increases in PGE-2, TNF-? IL-6 and IL-1? levels were also significantly prevented by indomethacin (10 mg/kg). UPLC-MS/MS facilitated the identification of 44 phenolic compounds, including phenolic acids and flavonoids. Conclusion: This is the first report of the chemical and biological study of the peels of Cucumis melo var. cantalupensis and Cucumis melo var. reticulatus. � 2019
Recent Updates in Pharmacological Properties of Chitooligosaccharides
(Hindawi Limited, 2019) Marmouzi I.; Ezzat, Shahira M; Salama M.M.; Merghany R.M.; Attar A.M.; El-Desoky A.M.; Mohamed S.O.; University Mohammed v in Rabat; Facult� de M�dicine et Pharmacie; Laboratoire de Pharmacologie et Toxicologie; �quipe de Pharmacocin�tique; Rabat; Morocco; Pharmacognosy Department; Faculty of Pharmacy; Cairo University; Kasr El-Ainy Street; Cairo; 11562; Egypt; Department of Pharmacognosy; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA); 6th October12611; Egypt; Department of Pharmacognosy and Medicinal Plants; Faculty of Pharmacy; British University in Egypt; Cairo; 11837; Egypt; Department of Pharmacognosy; National Research Centre; Giza; Egypt; Faculty of Science and Techniques; Hassan II University of Casablanca; Mohammedia; Morocco; Department of Molecular Biology; Genetic Engineering and Biotechnology Research Institute (GEBRI); University of Sadat City (USC); Sadat City; Egypt; Natural Wellness Scientific Office; Kuala Lumpur; Malaysia
Chemical structures derived from marine foods are highly diverse and pharmacologically promising. In particular, chitooligosaccharides (COS) present a safe pharmacokinetic profile and a great source of new bioactive polymers. This review describes the antioxidant, anti-inflammatory, and antidiabetic properties of COS from recent publications. Thus, COS constitute an effective agent against oxidative stress, cellular damage, and inflammatory pathogenesis. The mechanisms of action and targeted therapeutic pathways of COS are summarized and discussed. COS may act as antioxidants via their radical scavenging activity and by decreasing oxidative stress markers. The mechanism of COS antidiabetic effect is characterized by an acceleration of pancreatic islets proliferation, an increase in insulin secretion and sensitivity, a reduction of postprandial glucose, and an improvement of glucose uptake. COS upregulate the GLUT2 and inhibit digestive enzyme and glucose transporters. Furthermore, they resulted in reduction of gluconeogenesis and promotion of glucose conversion. On the other hand, the COS decrease inflammatory mediators, suppress the activation of NF-B, increase the phosphorylation of kinase, and stimulate the proliferation of lymphocytes. Overall, this review brings evidence from experimental data about protective effect of COS. � 2019 Ilias Marmouzi et al.