Browsing by Author "Salama, Maha M."

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Anti-Obesity Effect of a Tea Mixture Nano-Formulation on Rats Occurs via the Upregulation of AMP-Activated Protein Kinase/Sirtuin-1/Glucose Transporter Type 4 and Peroxisome Proliferator-Activated Receptor Gamma Pathways
(Multidisciplinary Digital Publishing Institute (MDPI), 2023-07) Salem, Mohamed A.; Aborehab, Nora M.; Abdelhafez, Mai M.; Ismail, Sameh H.; Maurice, Nadine W.; Azzam, May A.; Alseekh, Saleh; Fernie, Alisdair R.; Salama, Maha M.; Ezzat, Shahira M
White, green, and oolong teas are produced from the tea plant (Camellia sinensis (L.) Kuntze) and are reported to have anti-obesity and hypolipidemic effects. The current study aims to investigate the anti-obesity effects of a tea mixture nano-formulation by targeting the AMPK/Sirt-1/GLUT-4 axis in rats. In vitro lipase and α-amylase inhibition assays were used to determine the active sample, which was then incorporated into a nanoparticle formulation subjected to in vivo anti-obesity testing in rats by measuring the expression level of different genes implicated in adipogenesis and inflammation using qRT-PCR. Moreover, metabolomic analysis was performed for each tea extract using LC/ESI MS/MS coupled to chemometrics in an attempt to find a correlation between the constituents of the extracts and their biological activity. The in vitro pancreatic lipase and α-amylase inhibition assays demonstrated more effective activity in the tea mixture than the standards, orlistat and acarbose, respectively, and each tea alone. Thus, the herbal tea mixture and its nanoparticle formulation were evaluated for their in vivo anti-obesity activity. Intriguingly, the tea mixture significantly decreased the serum levels of glucose and triglycerides and increased the mRNA expression of GLUT-4, P-AMPK, Sirt-1, and PPAR-γ, which induce lipolysis while also decreasing the mRNA expression of TNF-α and ADD1/SREBP-1c, thereby inhibiting the inflammation associated with obesity. Our study suggests that the tea mixture nano-formulation is a promising therapeutic agent in the treatment of obesity and may also be beneficial in other metabolic disorders by targeting the AMPK/Sirt-1/Glut-4 pathway.
ANTI-WRINKLE ACTIVITY AND UPLC-MS/MS METABOLIC PROFILING OF POMEGRANATE AND GRAPE SEEDS EXTRACTS
(INT JOURNAL PHARMACEUTICAL SCIENCES, 08/01/2020) Odah, Sally M.; Salama, Maha M.; Aziz, Wafaa M.; El-Alfy, Taha S.; Ezzat, Shahira M
UV irradiation is one of the main causes of what is known as photoaging characterized by loss of skin elasticity and the appearance of wrinkles. In this study, the anti-wrinkle activity of grape seed extract (GSE) and Pomegranate seed extract (PSE) was studied through evaluation of their in-vitro antioxidant, anti-tvrosinase and in-vivo anti-wrinkle activity in UVB-irradiated mice through topical application of PSE and GSE at 50 mg/kg/twice a week for 15 weeks. The total phenolic content of PSE and GSE was 2.1375 and 5.9625 mg GAE/g extract, respectively. PSE and GSE also showed DPPH scavenging activity with IC50 of 37.9 and 29.9 mu g/mL, respectively. PSE and GSE showed anti-tyrosinase activity with IC50 of 115 and 107.75 mu g /mL, respectively, while that of Kojic acid is 17.45 mu g /mL. In UVB-irradiated mice, the increased expression of metaloproteinase (MMP-2) was significantly inhibited by PSE and GSE where their activities exceeded that of the standard turmeric extract (TME). The topical application of PSE, GSE, and TME of the UVB radiated groups prevented the increase in the thickness of the epidermis induced by UVB. UPLC-MS/MS profiling of both extracts led to the identification of 43 phenolic compounds of different classes. We can conclude that the anti-wrinkle activity of both extracts was attributed to their high phenolic content and the antioxidant potential of both extracts. Therefore, the seeds of pomegranate and grapes, which are considered as waste products, could be incorporated in cosmeceutical skincare products after further clinical trials
Upregulation of MC4R and PPAR-α expression mediates the anti-obesity activity of Moringa oleifera Lam. in high-fat diet-induced obesity in rats
(Elsevier Ireland Ltd, 01/01/2020) Ezzat, Shahira M; El Bishbishy, Mahitab H.; Aborehab, Nora M; Salama, Maha M.; Hasheesh, Adel; Abdel Motaal, Amira; Rashad, Hend; Metwally, Fateheya M
Ethnopharmacological relevance: various extracts of Moringa oleifera Lam. leaves, were reported to possess antiobesity effect in experimental animals models, yet its active doses and mechanism of action are still unclear. Materials and methods: The metabolic profiling of 70% ethanol extract of M. oleifera (MO) leaves was performed using HPLC-MS/MS analysis. The antiobesity activity of MO was tested in high fat diet induced obesity in rats at 200 and 400 mg/kg body weight orally for 1 month. Total cholesterol (TC), high density lipoproteins (HDL-C), low density lipoprotein-cholesterol (LDL-C), triglycerides (TGs), insulin resistance, insulin sensitivity, and adipose tissue index were monitored. In addition, fatty acid synthase (FAS) and HMG-CoA reductase mRNA from liver tissue, Peroxisome Proliferator-Activated Receptor alpha (PPARα) and Melanocortin-4 receptor (MC4R) RNA from adipose tissue were quantified using qRT-PCR. MO hard gelatin capsules (400 mg/capsule) were formulated and standardized using HPLC-RP analysis and tested on fifteen female participants, aged 45–55 with a BMI of 29–34 kg/m2. Results: Thirteen metabolites were tentatively identified using HPLC-MS/MS analysis including flavonols, flavones and a phenolic acid. MO 400 showed a prominent effect on reducing the rats’ final weights, % weight increase and adiposity index (P < 0.05). Glucose, insulin and HOMA-IR were significantly reduced and R-QUICKI was significantly increased by MO 400 (P < 0.001). Mean tissue level of leptin and vaspin were significantly reduced, adiponectin, omentin and GLUT-4 expression were increased significantly by MO 400 (P < 0.01). MO 400 significantly suppressed FAS and HMG-CoA reductase and increased mRNA expression of MC4R and PPAR-α (P < 0.01). Eight weeks administration of MO hard gelatin capsules to obese patients showed significant reduction of the average BMI, TC and LDL compared to the baseline values (p < 0.05). Conclusion: Our results presented a scientific evidence for the traditional use of M. oleifera leaves as antiobesity herbal medicine