Browsing by Author "Salah El Dine, Riham"

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New Bioactive Metabolites from a Crown Gall Induced on an Eucalyptus tereticornis Sm. Tree
(Zeitschrift für Naturforschung, 2013) Ezzat, Shahira M; M. Salama, Maha; Salah El Dine, Riham; M. El-Sayed, Aly; A. Sleem, Amany
Applying a bioactivity-guided isolation strategy for the ethanolic extract of crown gall tumours induced on an Eucalyptus tereticornis tree, two new compounds in addition to a known one were isolated. The new compounds were identifi ed as an amino acid derivative named 1-ethyl-6-(1'-methyl-1'-phenylethyl) piperidin-2-one (1) and a lanostane tetracyclic triterpene named 3β-hydroxy-24-methyllanosta-8,17(20),24(28)-trien-22-oic acid (2), together with stigmasterol-3-O-glucoside (3). The three compounds exhibited signifi cant cytotoxic activity against two human cell lines, breast (MCF7) and colon (HCT116), with IC50 values of 1.01, 1.54, and 2.15 μg/ml, respectively, against MCF7 and 3.49, 3.83, and 3.39 μg/ ml, respectively, against HCT116. Furthermore, in rats elevated levels of blood cholesterol, triglycerides, and low-density lipoprotein (LDLc) were signifi cantly reduced, while the level of high-density lipoprotein (HDLc) was signifi cantly increased by administration of the etha nolic extract as well as of 3. These results support a correlation between the reduction of blood cholesterol levels and improvement of colorectal cancer.
Protective effect of Echinops galalensis against CCl4-induced injury on the human hepatoma cell line (Huh7)
(Elsevier, 2013) Ezzat, Shahira M; M. Abdallah, Hossam; Salah El Dine, Riham; Abdel-Sattar, Essam; B. Abdel-Naim, Ashraf
Phytochemical investigation of the flowering aerial parts of Echinops galalensis (Asteraceae) led to the isolation of a new taraxasteryl triterpene, 3b-acetoxy-taraxast-12, 20(30)-diene-11a-21a-diol (1), together with nine known metabolites, a-amyrin (2), b-sitosterol (3), erythrodiol (4), lup-20(29)-ene- 1,3-diol (5), 1,5-dicaffeoylquinic acid (6), 3,5-dicaffeoylquinic acid (7), 3,4-dicaffeoylquinic acid (8), 4,5- dicaffeoylquinic acid (9) and apigenin-7-O-b-D-glucoside (10). The structure of the new compound was determined by comprehensive analyses of their 1D and 2D NMR, mass spectral (HR-EI) data and comparison with previously known analogs. The effect of the methanol extract of E. galalensis, its fractions as well as compounds (1–10) on human hepatoma cell line (Huh7) was evaluated according to aspartate aminotransferase (AST), alanine transaminase (ALT), superoxide dismutase (SOD) activities and malondialdehyde (MDA) level before and after exposure of the cells to carbon tetrachloride (CCl4). It was found that pre-treatment of human hepatoma cell line (Huh7) with the tested samples (100 mg/ml) prior to CCl4 challenge protected against cell injury. The protective effect of E. galalensis was suggested to be mediated, at least partly, by its antioxidant activity.
Valorization of Egyptian Food Byproducts in the Development of Biologically Active Nutraceuticals.
(IJP, 2018) Abdel-Sattar, Essam; Amer, Reham I.; Bagrel, Denyse; El Mansourai, Latifa; Philippot, Stéphanie; Darwish El-Tanbouly, Nebal; Evain-Bana, Emilie; Boukhira, Smahane; Abou-Hussein, Dina R.; Bousta, Dalila; Tzanova, Tzvetomira; Salah El Dine, Riham
This study was aimed to maximize the benefits of the use of by-products (oil cake) of olive fruits and black seeds after extraction of their fixed oils, rather than their use as animals feed or landfilling or composting. The cake of both seeds was assessed by HPLC for their main bioactive marker compounds (thymoquinone for black seed and oleuropein for olive fruit), to choose the best method for extraction, determination of phenolic contents and in vitro antioxidant activity (DPPH and reducing powers, FRAP assays). Both by-products were also assessed for their cytotoxicity against four human breast cell lines, three of them are cancerous (MCF7, MDA-MB-213, Vcr-R), and a non-cancerous one (epithelial type) but immortalized by telomerase (hTERT-HME), in addition to a human hepatoma cell line (HepG2). Also, both wastes were subjected for in-vitro CDC25s phosphatase inhibition assay on three isoforms (A-C) and for in-vivo immunomodulatory effects. In conclusion, the results of this study showed the interest of cumulating different biological approaches exploring various physiological mechanisms and showed the utility of these extracts in different fields, the first being used as a cytoprotective agent, and the second one being promising as an anticancer agent.