Browsing by Author "Sabet, Salwa"

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Adipocyte of Obese Breast Cancer Patients Is Characterized by The Overexpression of Caveolin-1 Protein/Mediator the Main Constituent of the Plasma Membrane Vesicles Caveolae That Contain Proteins Contribute to Breast Cancer Progression
(Egyptian Society of Biological Sciences, 2019) Saber, Aya; Abdelaziz Ibrahim, Sherif; Hosney, Mohamed; Taha Mohamed, Hossam; Fares, Mohamed; Sabet, Salwa; El-Shinawi, Mohamed; Mostafa Mohamed, Mona
Breast cancer (BC) is the second leading mortality cause due to poor survival rates compared to lung cancer all over the world. Recently, lifestyle increased obesity among the population globally. Since, the adipose tissues (AT) are the major contributor to the volume of the breast and adipocytes cells, which constitute AT are one of the major prominent cells play an effective role in cancer progression via releasing different mediators and adipokines. Thus, AT may display a crucial role in BC progression, especially in obese patients compared to non-obese patients, which characterized by increased AT. Interestingly, adipocytes are characterized by expressing caveolin-1 (Cav-1) protein. Cav-1 constitutes the lipid raft of caveola which contains different proteolytic enzymes inducing cancer metastasis. In this regard, the aim of the present study was to explore the level of expression of Cav-1 protein in the tissue specimen of 5 non-obese vs. 15 obese patients using immunohistochemistry (IHC) and immunoblotting techniques. Our finding demonstrates that the level of Cav-1expression was statistically significantly low in non-obese compared to obese BC patients (p < 0.05). Herein, our results revealed that the highest expression of Cav-1 in obese patients compared to non-obese (control) patients can be considered as a biomarker for BC patients.
Chalcones: Promising therapeutic agents targeting key players and signaling pathways regulating the hallmarks of cancer
(Elsevier Ireland Ltd, 2023-01) WalyEldeen, Amr Ahmed,; Sabet, Salwa; El-Shorbagy, Haidan M.; Abdelhamid, Ismail A; Ibrahim, Sherif Abdelaziz
The need for innovative anticancer treatments with high effectiveness and low toxicity is urgent due to the development of malignancies that are resistant to chemotherapeutic agents and the poor specificity of existing anticancer treatments. Chalcones are 1,3-diaryl-2-propen-1-ones, which are the precursors for flavonoids and isoflavonoids. Chalcones are readily available from a wide range of natural resources and consist of very basic chemical scaffolds. Because the ease with which the synthesis it allows for the production of several chalcone derivatives. Various in-vitro and in-vivo studies indicate that naturally occurring and synthetic chalcone de- rivatives exhibit promising biological activities against cancer hallmarks such as proliferation, angiogenesis, invasion, metastasis, inflammation, stemness, and regulation of cancer epigenetics. According to their structure and functional groups, chalcones derivatives and their hybrid compounds exert a broad range of biological ac- tivities through targeting key elements and signaling molecules relevant to cancer progression. This review will provide valuable insights into the latest updates of chalcone groups as anticancer agents and extensively discuss their underlying molecular mechanisms of action.
Fibulin-2 is required for basement membrane integrity of mammary epithelium
(Nature Publishing Group, 2018) M Ibrahim, Ayman; Sabet, Salwa; A El-Ghor, Akmal; Kamel, Nora; E Anis, Shady; S Morris, Joanna; Stein, Torsten
Fibulin-2 (FBLN2) is a secreted extracellular matrix glycoprotein which has been associated with tissue development and remodelling. In the mouse mammary gland, FBLN2 can be detected during ductal morphogenesis in cap cells and myoepithelial cells at puberty and early pregnancy, respectively. In an attempt to assign its function, we knocked down Fbln2 in the mouse mammary epithelial cell line EpH4. FBLN2 reduction led to an increase in the size of spheroidal structures when compared to scrambled control shRNA-transduced cells plated on Matrigel matrix. This phenotype was associated with a disruption of the collagen IV sheath around the epithelial spheroids and downregulation of integrin β1, suggesting a role for FBLN2 in stabilizing the basement membrane (BM). In contrast to mice, in normal adult human breast tissue, FBLN2 was detected in ductal stroma, and in the interlobular stroma, but was not detectable within the lobular regions. In tissue sections of 65 breast cancers FBLN2 staining was lost around malignant cells with retained staining in the neighbouring histologically normal tissue margins. These results are consistent with a role of FBLN2 in mammary epithelial BM stability, and that its down-regulation in breast cancer is associated with loss of the BM and early invasion.
Inflammatory breast cancer: High incidence of GCC haplotypes (−1082A/G, −819T/C, and −592A/C) in the interleukin-10 gene promoter correlates with over-expression of interleukin-10 in patients’ carcinoma tissues
(SAGE Publications, 2017) Sabet, Salwa; Khalaf El-Sayed, Shrouk; Taha Mohamed, Hossam; El-Shinawi, Mohamed; M. Mohamed, Mona
Interleukin-10 is involved in carcinogenesis by supporting tumor escape from the immune response. The aim of this study was to assess the single nucleotide polymorphisms, −1082A/G, −819T/C and −592A/C, in interleukin-10 gene promoter in inflammatory breast cancer compared to non–inflammatory breast cancer and association of these polymorphisms with interleukin-10 gene expression. We enrolled 105 breast cancer tissue (72 non–inflammatory breast cancer and 33 inflammatory breast cancer) patients and we determined the three studied single nucleotide polymorphisms in all samples by polymerase chain reaction restriction fragment length polymorphism and investigated their association with the disease and with various prognostic factors. In addition, we assessed the expression of interleukin-10 gene by realtime quantitative reverse transcription polymerase chain reaction and the correlation between studied single nucleotide polymorphisms and interleukin-10 messenger RNA expression. We found co-dominant effect as the best inheritance model (in the three studied single nucleotide polymorphisms in non–inflammatory breast cancer and inflammatory breast cancer samples), and we didn’t identify any association between single nucleotide polymorphisms genotypes and breast cancer prognostic factors. However, GCC haplotype was found highly associated with inflammatory breast cancer risk (p<0.001, odds ratio=43.05). Moreover, the expression of interleukin-10 messenger RNA was significantly higher (p<0.001) by 5.28-fold and 8.95-fold than non–inflammatory breast cancer and healthy control, respectively, where GCC haplotype significantly increased interleukin-10 gene expression (r=0.9, p<0.001).
Resveratrol and Gold Nanoparticles Combination Downregulate Livin in Hepatocellular Carcinoma Rat Model
(NIDOC (Nat.Inform.Document.Centre), 2024-05) Bassiony, Heba; Elsheikh, Sahar; Shahein, Yasser Ezzat; Sabet, Salwa; ElShorbagy, Haidan M
Hepatocellular carcinoma (HCC) has reported high prevalence rates recently in Egypt and worldwide. Livin is one of the apoptotic inhibitor genes and its silencing increases the apoptotic incidence and hence the resistance to cancer proliferation. Resveratrol (Res) and gold nanoparticles (Au-NPs) have been documented for their antitumor activities. In the present study, we used hepatocarcinoma as a model to investigate the combination of Res and Au-NPs (Res-Au-NPs) antitumor activity, via their effect on the expression of livin’ and A-fetoprotein genes (AFP). Au-NPs (size: 50.0±2.0 nm) were produced by the seeded growth technique. The HCC rat model was administered with Res, Au-NPs, and their combination for two months. Livin and AFP gene expressions, DNA damage, and pathological changes in the liver tissues were assessed. Our results revealed a significant reduction in livin’ and AFP mRNA expression levels in HCC rats treated with a combination of Res and Au-NPs compared to those treated with Res or Au-NPs alone. This reduction was accompanied by increased DNA damage in the same group. The histological examination revealed an improvement in parenchymal structure with decreased degenerated hepatocytes in the Res-Au-NPs treated group compared to HCC, Res, and Au-NPs groups. In conclusion, the combination of Res and Au-NPs might be more effective in HCC treatment than using Res or Au-NPs alone due to the synergetic effects of both, which might be a prospective directed approach for the treatment of HCC.
Silica-coated graphene compared to Si-CdSe/ZnS quantum dots: toxicity, emission stability, and role of silica in the uptake process for imaging purposes
(Elsevier, 2022-05) ElZorkany, Heba ElSayed; Farroh, Khaled Yehia; El-Shorbagy, Haidan M; Elshoky, Hisham A; Youssef, Tareq; Salaheldin, Taher A; Sabet, Salwa
Quantum dots (QDs) present a special type of nanocrystals (NCs) due to their unique optical and chemical properties. While cadmium- based QDs (Cd-QDs) have the most favorable physicochemical properties, their toxicity, instability in the aqueous phase, and loss of brightness at high temperature are some of the obstacles that prevent the wide use of Cd-QDs. Carbon-based QDs as graphene quantum dots (GQDs) represent a very promising biocompatible replacement. In the present work, we mainly focus on comparing the efficiency and uptake of GQDs and Cd-QDs for fluorescent imaging purposes and studying the. effect of growing silica shell on the emission and the uptake of QDs inside living human and bacterial cells. Graphene and CdSe/ZnS QDs were prepared and encapsulated in silica to increase their emission and uptake by living cells. Moreover, we studied their photostability and cytotoxicity. The Prepared G-Si QDs showed good emission inside the cytoplasmic portion of the liver hepatocellular carcinoma cell line (HepG2) and Bacillus subtilis (B. subtilis), but they revealed lower photoluminescence (PL) intensity compared to Si-CdSe/ZnS NCs although G-Si QDs are advantageous in other aspects, i.e. possess lower toxicity and higher stability with temperature variations.
Targeting Apollon via siRNA Induces Apoptosis in Different Human Malignancies
(IDOSI Publications, 2014) G Mohamed, Layaly; Sabit, Hussein; Sabet, Salwa; El-Sayed Salem, Reda; El-Ghareeb, Abdel-Wahab
Apollon, also called Baculoviral IAP Repeat-Containing Protein 6 (BIRC6) or Baculoviral IAP Repeat-containing Ubiquitin Conjugating Enzyme (BRUCE), is an anti-apoptotic protein belonging to the IAP family, which consists of eight members. The genes of this family render cancer cells insensitive to apoptotic stimulation. The aim of the present study was to investigate and assess the role of small interference RNA (siRNA) in the regulation of Apollon gene expression in four different human cancerous cell lines; breast cancer (MCF-7), cervical cancer (HeLa), colon cancer (CaCo-2) and hepatocellular carcinoma (HepG-2). Lipofection was carried out to introduce the Apollon-specific siRNA into the cancerous cells and the Apollon expression levels were determined using RT-PCR. Trypan blue assay was conducted to assess the integrity of the cell membranes after being transfected. 3-(4, 5-dimethylthiazol-2-yl)-2-5- Diphenyl tetrazolium bromide (MTT) assay was also implemented to assess the cell viability through the mitochondrial reductase enzymes activity. The obtained results concluded that transfecting the malignant cells with Apollon-specific siRNA have led to the down regulation of Apollon expression compared to the control non-transfected cells. RNA interference targeting the anti-apoptotic genes such as Apollon could be considered as a promising approach and may help as a future therapeutic tool for many types of human cancers.