Browsing by Author "S. El-Hawary, Seham"

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Comparative Botanical Study and DNA Finger Printing of Certain Eugenia Species Growing in Egypt
(International Journal of Pharmaceutical and Clinical Research, 2016) Ezzat, Shahira M; S. El-Hawary, Seham; F. Taha, Kamilia; Y. Eissa, Amira
Objective: to discriminate between six Eugenia species growing in Egypt based on their micro-morphological and genetic characterization. Methods: For establishment of different botanical and genetic criteria, this study presents a comparative investigation of the botanical features of the leaves and stems through microscopically investigation of the prepared entire, transverse sections and isolated elements of these organs. Furthermore, the DNA of the six Eugenia species was extracted from leaf samples and Inter Simple Sequence Repeat (ISSR) analysis using six primers of arbitrary sequences. Results: Comparative botanical characters of the different organs were identified. On the other hand, the total number of amplified product was 68 fragments, which were generated by the six primers; the primer HB-09 recorded 55.5% as the highest percentage of polymorphism. As well as, the primer HB-44 recorded 54.5% polymorphism. The primer HB-15 recorded the lowest percentage of polymorphism (10%) and the highest degree of similarity (90%). Conclusion: For the present study, microscopical characters, as well as, DNA fingerprinting can be considered as the identifying parameters to authenticate and differentiate between the six Eugenia species under study.
Effect of Certain Essential oils on Dissolution of Three Commercial Gutta-percha Brands
(Journal of Essential Oil Bearing Plants, 2015) Ezzat, Shahira M; S. El-Hawary, Seham; E. Eid, Gehan; K. Abd-El Rhman, Shaimaa
The purpose of this study was to test the ability of some essential oils to dissolve three types of commercial gutta-percha brands which are commonly used in Egypt. The chemical composition of three commercially available gutta-percha brands (Hygenic, Maillefer and Meta) was determined using X-ray, FTIR and elemental microanalysis. Essential oils from thirty medicinal plants representing the six most famous families of aromatic plants growing in Egypt were extracted by steam distillation and tested for dissolving the gutta-percha brands. Essential oils from the peels of Citrus x sinensis (L.) Osbeck (Navel orange), C. deliciosa Tenora (Common mandarin) and C. x aurantium L.(Bitter orange)(Rutaceae) showed the highest dissolution rate. The essential oils from the aerial parts of Origanum syriacum L. and Lavandula hyprida Reverchon cv. Grosso (Labiateae) showed complete dissolution at 40oC, also essential oils from the seeds of Elletaria cardamomum (Cardamom) Matonvar. miniscula Burkill (Zingibraceae) showed similar dissolution at 40oC. GC-MS analysis was performed on six essential oils representing the three effective families in order to correlate their composition with their dissolution efficiency.
Evaluation of the Phenolic and Flavonoid Contents, Antimicrobial and Cytotoxic Activities of Some Plants Growing in Al Jabal Al-Akhdar in Libya
(International Journal of Pharmacognosy and Phytochemical Research, 2016) Ezzat, Shahira M; S. El-Hawary, Seham; El Shabrawy, Abd El Rahman; A. A. El-Shibani, Fatma
The methanolic extract of the aerial part of two Libyan medicinal plants Arbutus pavarii. Pampan (Ericaceae) and Sarcopoterium spinosium. L. (Rosaceae) growing in El-Jabal Al Akhdar area were studied for their phenolic and flavonoid content, antimicrobial and cytotoxic activitie. Total polyphenol contents ranged from 61.7±2.7to 163.6±0.85 μg gallic acid equivalent / g (A. pavarii Pampan and S. spinosium. L.) and total flavonoid contents ranged from 126.9±2.98 to 206.1±1.09 μg rutin equivalent (A. pavarii Pampan and S. spinosium L.). Qualitative and quantitative analysis of major phenolic and flavonoids in the extracts were conducted by high-performance liquid chromatography (HPLC). Finally, antimicrobial activities of the two plants were measured using the disc diffusion method. While, cytotoxic properties were tested against the HEPG2 and T47D cell lines. Arbutus pavarii extract proved to be the most cytotoxic extract in this study with IC50 19.7±2.8 and 19±0.65 (μg/ml) on HEPG2 and T47D respectively.