Browsing by Author "Rashad, Hend"

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    Moringa oleifera offers a Multi-Mechanistic Approach for Management of Obesity in Rats
    (International Journal of Pharmaceutical Sciences Review and Research, 2014) H. Ahmed, Hanaa; M. Metwally, Fateheya; Rashad, Hend; M. Zaazaa, Asmaa; M. Ezzat, Shahira; M. Salama, Maha
    Obesity is a condition in which excess body fat is accumulated to an extent that health may be negatively affected. Obesity is associated with a number of chronic health problems such as diabetes, heart disease, hypertension and cancer. The current study was constructed to evaluate the efficacy of alcoholic extract of Moringa oleifera in management of obesity induced by high cholesterol diet in rats. Adult female albino rats were classified into four groups. The first group was kept on standard rodent chow for 30 weeks (lean control). The other three groups received high cholesterol diet for 30 weeks. These animals were assigned as obese control group, Moringa oleifera treated group and Simvastatin treated group. The results revealed significant increase in thoracic (TC) and abdominal (AC) circumferences as well as body mass index (BMI) in obese group. Moreover, dyslipidemia, and hyperleptinemia have been demonstrated in obese group. Furthermore, serum malondialdehyde (MDA) and nitric oxide (NO) levels were significantly increased in obese group versus the lean control group. In addition, obese group revealed significant decrease in serum adiponectin level in concomitant with significant increase in resistin level as compared to lean control group. On the other side, treatment with Moringa oleifera alcoholic extract or simvastatin could reduce food intake and BMI as well as ameliorate the dyslipidemia, in obese groups. Serum leptin level showed significant decrease in obese group due to treatment with Moringa oleifera alcoholic extract or Simvastatin. As well significant inhibition of serum MDA and NO levels was detected as a consequence of treatment with either Moringa oleifera extract or Simvastatin. Additionally, the treatment of obese group with Moringa oleifera extract or Simvastatin resulted in significant decrease in serum resistin level in concomitant with significant increase in serum adiponectin level as compared to obese control group. In conclusion, the data of the current study provides experimental evidence for the anti-obesity effect of Moringa oleifera ethanol extract. Thus, present findings reinforce the advice recommending consumption of Moringa oleifera to modulate obesity.
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    Randomized double-blinded pilot clinical study of the antidiabetic activity of Balanites aegyptiaca and UPLC-ESI-MS/MS identification of its metabolites
    (Taylor & Francis, 2017) Rashad, Hend; M Metwally, Fateheya; Ezzat, Shahira M; M Salama, Maha; Hasheesh, Adel; Abdel Motaal, Amira
    Balanites aegyptiaca Del. (Zygophyllaceae) fruits are traditionally known for the treatment of hyperglycaemia. Several in vitro and in vivo studies proposed some mechanisms of action. However, clinical trials in human beings were never reported to date.
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    Upregulation of MC4R and PPAR-α expression mediates the anti-obesity activity of Moringa oleifera Lam. in high-fat diet-induced obesity in rats
    (Elsevier Ireland Ltd, 01/01/2020) Ezzat, Shahira M; El Bishbishy, Mahitab H.; Aborehab, Nora M; Salama, Maha M.; Hasheesh, Adel; Abdel Motaal, Amira; Rashad, Hend; Metwally, Fateheya M
    Ethnopharmacological relevance: various extracts of Moringa oleifera Lam. leaves, were reported to possess antiobesity effect in experimental animals models, yet its active doses and mechanism of action are still unclear. Materials and methods: The metabolic profiling of 70% ethanol extract of M. oleifera (MO) leaves was performed using HPLC-MS/MS analysis. The antiobesity activity of MO was tested in high fat diet induced obesity in rats at 200 and 400 mg/kg body weight orally for 1 month. Total cholesterol (TC), high density lipoproteins (HDL-C), low density lipoprotein-cholesterol (LDL-C), triglycerides (TGs), insulin resistance, insulin sensitivity, and adipose tissue index were monitored. In addition, fatty acid synthase (FAS) and HMG-CoA reductase mRNA from liver tissue, Peroxisome Proliferator-Activated Receptor alpha (PPARα) and Melanocortin-4 receptor (MC4R) RNA from adipose tissue were quantified using qRT-PCR. MO hard gelatin capsules (400 mg/capsule) were formulated and standardized using HPLC-RP analysis and tested on fifteen female participants, aged 45–55 with a BMI of 29–34 kg/m2. Results: Thirteen metabolites were tentatively identified using HPLC-MS/MS analysis including flavonols, flavones and a phenolic acid. MO 400 showed a prominent effect on reducing the rats’ final weights, % weight increase and adiposity index (P < 0.05). Glucose, insulin and HOMA-IR were significantly reduced and R-QUICKI was significantly increased by MO 400 (P < 0.001). Mean tissue level of leptin and vaspin were significantly reduced, adiponectin, omentin and GLUT-4 expression were increased significantly by MO 400 (P < 0.01). MO 400 significantly suppressed FAS and HMG-CoA reductase and increased mRNA expression of MC4R and PPAR-α (P < 0.01). Eight weeks administration of MO hard gelatin capsules to obese patients showed significant reduction of the average BMI, TC and LDL compared to the baseline values (p < 0.05). Conclusion: Our results presented a scientific evidence for the traditional use of M. oleifera leaves as antiobesity herbal medicine