Browsing by Author "Nour, Mai S"

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Green and white-assessed validated chromatographic methods for Ondansetron purity testing in its pharmaceutical formulations; in silico toxicity profiling of impurities
(Elsevier Inc, 2024-02) El-Maraghy, Christine M; Nour, Mai S; ELbalkiny, Heba T
Drug impurities are seen as a crucial threat to drug safety, specifically when dealing with mutagenic/ toxic impurities. Here, we present LC-MS/MS and HPTLC-densitometric methods for simultaneous quantification of Ondansetron and its four official impurities. For the LC-MS/MS, the isocratic elution was applied using methanol and water containing 0.1 % formic acid in a ratio (70:30 v/v) at a flow rate of 1 mL/min, a stationary phase C18 column (4.6 × 50 mm, 5 µm) and mass detection using the MRM mode. For the HPTLC-densitometric method, the mobile phase consists of ethyl acetate: methanol in a ratio (6:4 v/v), and the UV detection was at 216 nm. The developed methods have been validated per ICH recommendations and then evaluated using five tools for whiteness and greenness assessment, offering promising results in comparison to reported chromatographic methods. Additionally, the toxicity profile of the impurities was expected by the online software; PreADMET and pkCSM. The developed methods are recommended for quality control due to their high analytical performance as well as their sustainability, simplicity, and cost-effectiveness, which improves the surveillance capability.
Sterols from Centaurea pumilio L. with cell proliferative activity: In vitro and in silico studies
(2023-04) Fayed, Marwa A. A; Al-Wahaibi, Lamya H; Bakr, Riham O; Nour, Mai S; Basudan, Omer A; Parvez, Mohammad K; Al-Dosari, Mohammed S; Abdel-Mageed, Wael M
Numerous studies highlighted the impact of natural products, particularly phytosterols, in wound healing while providing less expensive alternatives to che- mically synthesized drugs, with less side effects. Centaurea pumilio L. (family Asteraceae) is a rare and endangered species of genus Centaurea with few reports concerning its chemistry. Our phytochemical investigation for the non-polar fraction of its aerial parts led to the isolation and identification of the new compound (6) identified as stigmast-1,5-dien-3-O-β-D-glucopyranoside along with five known sterols and triterpenes (1–5) identified as taraxas- terol, β-sitosterol, stigmasterol, β-sitosterol glucoside, and stigmasterol-3-O-β-D-glucopyranoside. Structures of the isolated compounds have been characterized using 1D, 2D NMR, and mass spectral analyses. The cell viability and proliferative activity of the isolated compounds were evaluated using an MTT assay on cultured human primary umbilical vein endothelial cells (HUVEC). None of the com- pounds exhibited any sign of cytotoxicity. Nonetheless, com- pounds 5 and 6 moderately enhanced the HUVEC cell growth by 14 and 16%, respectively, at the maximal tested dose (50 µg/mL). As inhibition of glycogen synthase kinase 3-β (GSK3-β) enzyme is important to enhance the wound healing process; therefore, molecular docking was per- formed to understand the possible interactions between bioactive compounds 5 and 6 and GSK-3β binding pocket active amino acid residues. Both compounds were able to bind to the substrate‑binding site of GSK-3β and poten- tially interact with the key active site residues, forming strong π and hydrogen interactions with the catalytic site residues, revealing lower binding energy (−7.185 and −6.303 kcal/mol, respectively) than that of indirubin-3- monooxime (−5.303 kcal/mol); thereby representing strong natural replacements candidates for GSK-3β inhibitors