Browsing by Author "Mostafa, Ahmed"

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Antiviral potential of rosuvastatin and hesperidin in combination with favipiravir liposomal nanoformulations in targeting the main protease (M pro) of SARS-CoV-2: Molecular docking, molecular dynamics and in-vitro studies
(Editions de Sante, 2024-05) Elimam, Hanan; El-Sawy, Hossam S; Fayed, Marwa A.A; Mahmoud, Sara H; Bakr, Riham O; Saleh, Rasha M; Mostafa, Ahmed; Elshal, Mohamed F
Favipiravir (Fav) is a drug utilized to treat coronavirus disease 2019 (COVID-19) due its capacity to expedite the clearance of the SARS-CoV-2 virus through binding to its main protease (Mpro). However, the use of Fav has been associated with some adverse health effects. Meanwhile, numerous studies have highlighted the potential antiviral activities of specific phytochemicals and statins. Consequently, we thought to explore drug combination strategies involving certain statins and phytochemicals and their liposome nanoformulations either alone or with Fav, aiming to augment its efficacy and mitigate potential adverse effects. The molecular docking and molecular dynamic simulations analyses have revealed that hesperidin (HES) and rosuvastatin (ROS) have the best targeting potential for Mpro protein out of 10 phytochemicals and 6 statin compounds. The selected compounds were elaborated alone or with FAV into six nanoformulations FAV, ROS, HES, FAV/ROS, FAV/HES, and FAV/ ROS/HES-loaded liposomes. Light and electron microscope evaluations confirmed the vesicular shape of all liposomal dispersions. The entrapment capacity and release extent from FAV/ROS/HES-loaded liposomes was the lowest compared to other nanoformulations. In vitro, the FAV/HES or FAV/ROS-loaded liposomes displayed the highest capacity to impede the replication of SARS-CoV-2 with IC50 of 0.738 and 3.28 μg/mL, respectively. These results confirmed the potential of hesperidin and rosuvastatin as adjuvant medications with Favipiravir to combat COVID-19 and suggest the preference of the combinatory treatments. Finally, our findings provide a rational for further in-vivo studies to evaluate the potential activities of these drug combinations to mitigate the adverse events of favipiravir and to boost its SARS-CoV-2 clearance efficacy.
Delineating a potent antiviral activity of Cuphea ignea extract loaded nano-formulation against SARS-CoV-2: In silico and in vitro studies
(Elsevier, 9/15/2021) Mahmoud, Dina B; Ismail, Walaa M; Moatasim, Yassmin; Kutkat, Omnia; ElMeshad, Aliaa N; Ezzat, Shahira M; El Deeb, Kadriya S; El-Fishawy, Ahlam M; Gomaa, Mokhtar R; Kandeil, Ahmed; Al-karmalawy, Ahmed A; Ali, Mohamed A; Mostafa, Ahmed
Journal of Drug Delivery Science and Technology Available online 15 September 2021, 102845 In Press, Journal Pre-proofWhat are Journal Pre-proof articles? Delineating a potent antiviral activity of Cuphea ignea extract loaded nano-formulation against SARS-CoV-2: In silico and in vitro studies Author links open overlay panelDina B.MahmoudaWalaa M.IsmailbYassminMoatasimcOmniaKutkatcAliaa N.ElMeshaddeShahira M.EzzatbfKadriya S.El DeebbAhlam M.El-FishawybMokhtar R.GomaacAhmedKandeilcAhmed A.Al-karmalawygMohamed A.AlicAhmedMostafac a Pharmaceutics Department, National Organization for Drug Control and Research, Giza, Egypt b Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Kasr-El-Ainy Street, Cairo, 11562, Egypt c Center of Scientific Excellence for Influenza Viruses, National Research Centre, Giza, Egypt d Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Cairo University, Kasr-El-Ainy Street, Cairo, 11562, Egypt e Faculty of Nanotechnology for Postgraduate Studies, Cairo University, El Sheikh Zayed, Giza, 12588, Egypt f Department of Pharmacognosy, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), Giza, 12451, Egypt g Department of Pharmaceutical Medicinal Chemistry, Faculty of Pharmacy, Horus University-Egypt, New Damietta, 34518, Egypt Received 7 March 2021, Revised 17 July 2021, Accepted 5 September 2021, Available online 15 September 2021. https://doi.org/10.1016/j.jddst.2021.102845 Get rights and content Abstract The outbreak of coronavirus disease-2019, caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is a worldwide emerging crisis. Polyphenols are a class of herbal metabolites with a broad-spectrum antiviral activity. However, most polyphenols encounter limited efficacy due to their poor solubility and degradation in neutral and basic environments. Thus, the effectiveness of their pharmaceutical application is critically dependent on the delivery systems to overcome the aforementioned drawbacks. Herein, Polyphenols-rich Cuphea ignea extract was prepared and its constituents were identified and quantified. Molecular docking was conducted for 15 compounds in the extract against SARS-CoV-2 main protease, among which rutin, myricetin-3-O-rhamnoside and rosmarinic acid depicted the most promising antiviral activity. Further, a self-nanoemulsifying formulation, composed of 10% oleic acid, 40% tween 20 and propylene glycol 50%, were prepared to improve the solubility of the extract components and enable its concurrent delivery permitting combined potency. Upon dilution with aqueous phases, the formulation rapidly form nanoemulsion of good stability and excellent dissolution profile in acidic pH when compared to the crude extract. It inhibited SARS-CoV-2 completely in vitro at a concentration as low as 5.87 μg/mL presenting a promising antiviral remedy for SARS-CoV-2, which may be attributed to the possible synergism between the extract components.
Monitoring the Role of Programmed Cell Death 1 and its Ligands in Autoimmune Systemic Lupus Erythematous Disease
(MSA university Faculty of pharmacy, 2020) Mostafa, Ahmed; Ezzat, Eman; Mohsen, Mayar; Abdalaa, Walaa
Autoimmune diseases areone of the recent dangerous issues that occur without specific known reasons. This condition the immune system start to attack the host cells rather than protect the body from any harmful invasion considering these cells as a foreign substance needed to be attack. The reasons behind the falling of immune system to recognize the difference between the host cells and the foreign cells are unknown and they may be genetic, environmental, bad life style, uncontrolled diet or physiological reason. systemic lupus erythematosus (SLE) is one of the autoimmune diseases that mostly affect women in which the immune system start to attack the cells of the body result in many serious damage to different organs especially to skin, kidneys, liver and heart. For the diagnosis of SLE there are different assessment methods;either non-specific such as the serological test and the measuring of anti-dsDNA concentration in blood which increase with the presence of the disease or specific measuring through measuring the concentration of percentages of PD-1(program cell death that express on both CD3 T cells and CD19 B cells) and percentages of PD-L1(program cell death ligand that express on CD19 B cells).Both levelscan be measured using flow cytometer. Flow cytometer is an approach used to measure and detect various properties of the cells released to promote down regulation of immune system leading to of the autoimmune problem as a defense mechanism from the body against the up normal attacking of the immune system
Newly synthesized series of oxoindole–oxadiazole conjugates as potential anti-SARS-CoV-2 agents: in silico and in vitro studies
(Royal society of chemistry, 04/02/2022) El-Masry, Rana M; Al-Karmalawy, Ahmed A; Alnajjar, Radwan; Mahmoud, Sara H; Mostafa, Ahmed; Kadry, Hanan H; Abou-Seri, Sahar M; Taher, Azza T
In this study, a series of 1,3,4-oxadiazoles carrying the isatin moiety (IVa–g) as anti-SARS-CoV-2 agents were designed and synthesized. Molecular docking of the compounds (IVa–g) into the SARS-CoV-2 Mpro active site showed promising binding affinities. The docking results were supported using molecular dynamics simulations and MM-GBSA calculations as well. To validate the in silico predictions, all compounds were evaluated for their half-maximal cytotoxicity (CC50) and virus-inhibitory (IC50) concentrations. The CC50 concentrations were remarkably high for most of the tested compounds. However, compounds IVe and IVg showed high activity against SARS-CoV-2 at IC50 values of 13.84 μM and 4.63 μM, with selectivity indices of 4.1 and 5.9, respectively. The most potent antiviral agent IVg demonstrated an IC50 of 16.6 μM against SARS-CoV-2 Mpro, which is considered a moderate activity. However, the represented cellular antiviral activity of IVg could justify further optimization to develop this series of compounds as broad-spectrum anti-SARS-CoV-2 agents.
Simultaneous quantitative analysis of tamsulosin and finasteride in pharmaceutical dosage form by U-HPLC Tandem mass spectrometry
(European Journal of Chemistry, 2014) Mohamed, Dalia; Mowaka, Shereen; Mostafa, Ahmed
A sensitive, rapid, selective and accurate liquid chromatography coupled to quadrupole tandem mass spectrometry (LC-MS/MS) method was developed for simultaneous identification and quantification of tamsulosin and finasteride in bulk and in their combined dosage form. Chromatography was performed on a Hypersil gold 50 mm × 2 mm (1.9 μm) column, using acetonitrile:ammonium acetate (90:10, v:v) pH = 3.5 as the mobile phase. Protonated ions formed by a turbo ion-spray in positive mode were used to detect the analytes as well as the internal standard (IS). MS/MS detection was carried out by monitoring the fragmentation of 408.74 → 227.29 (m/z), 373.11 → 304.96 (m/z) and 255.75 → 166.15 (m/z) for tamsulosin, finasteride and diphenhydramine (IS), respectively, on a triple quadrupole mass spectrometer. The linearity was obtained over the concentration range of 1.6-40.0 ng/mL for tamsulosin and 20.0-500.0 ng/mL for finasteride with a lower limit of detection of 0.5 ng/mL and 5.0 ng/mL for the two drugs, respectively. The proposed method was successfully applied to tamsulosin and finasteride determination in pharmaceutical dosage form. The results obtained were statistically analyzed and compared with those of reference ones; in addition, the method was validated according to USP 34 recommendations. The simplicity and sensitivity of this method allows its use in the quality control of the cited drugs and can be extended for routine analysis of the drugs in their pharmaceutical preparations.