Browsing by Author "Matboli M."

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    Dual approach for the colorimetric determination of unamplified microRNAs by using citrate capped gold nanoparticles
    (Springer-Verlag Wien, 2018) Nossier A.I.; Abdelzaher H.; Matboli M.; Eissa S.; Biochemistry Department; Faculty of Pharmacy; Misr University for Science and Technology (MUST); 6th October City; Giza; Egypt; Faculty of Biotechnology; October University for Modern Sciences & Arts; 6th October City; Cairo; Egypt; Oncology Diagnostic Unit; Medical Biochemistry & Molecular Biology Department; Faculty of Medicine; Ain Shams University; Cairo; Egypt; Faculty of Medicine Ain Shams Research Institute (MASRI); Cairo; Egypt
    The authors describe a method for the colorimetric determination of unamplified microRNA. It is based on the use of citrate-capped gold nanoparticles (AuNPs) and, alternatively, a microRNA-probe hybrid or a magnetically extracted microRNA that serve as stabilizers against the salt-induced aggregation of AuNPs. The absorbance ratios A525/A625 of the reacted AuNP solutions were used to quantify the amount of microRNA. The assay works in the range of 5�25�pmol microRNA. The lower limit of detection (LOD) is 10�pmol. The performance of the method was tested by detection of microRNA-210-3p in totally extracted urinary microRNA from normal, benign, and bladder cancer subjects. The sensitivity and specificity for qualitative detection of urinary microRNA-210-3p using the assay are 74% and 88% respectively, which is consistent with real time PCR based assays. The assay was applied to the determination of specific microRNA by using its specific oligo targeter or following magnetic isolation of the desired microRNA. The method is simple, cost-efficient, has a short turn-around time and requires minimal equipment and personnel. � 2018, Springer-Verlag GmbH Austria, part of Springer Nature.
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    Investigating miRNA-661 and ATG4-B mRNA expression as potential biomarkers for hepatocellular carcinoma
    (Future Medicine Ltd., 2018) Ali M.A.; Matboli M.; El-Khazragy N.; Saber O.; El-Nakeep S.; Abdelzaher H.M.; Shafei A.E.-S.; Mostafa R.; Department of Biomedical Research; Armed Forces College of Medicine (AFCM); Cairo; 11774; Egypt; Department of Medical Biochemistry and Molecular Biology; Ain Shams Faculty of Medicine Research Center (Masri)11778; Egypt; Department of Clinical Pathology; Oncology Diagnostic Unit; Faculty of Medicine; Ain Shams University; Cairo; 11778; Egypt; Armed Forces College of Medicine (AFCM); Cairo; 11774; Egypt; Hepatology and Gastroenterology Unit; Internal Medicine Department; Faculty of Medicine; Ain Shams University; Cairo; 11778; Egypt; Faculty of Biotechnology; October University for Modern Sciences and Arts; Cairo; 12585; Egypt
    Aim: We aimed to examine the statistical association between serum expression of miRNA 661 (miR-661) and ATG-4B mRNA and hepatocellular carcinoma (HCC) based on in silico data analysis followed by clinical validation. Patients & methods: Quantitative reverse-transcriptase real-time PCR was used to examine the expression of miR-661 and ATG-4B mRNA in the sera of HCC patients versus control. Results: The expression of miR-661 and ATG-4B mRNA was positive in 97.14 and 77.14%, respectively, in HCC patients. The survival analysis showed that ATG-4B mRNA was an independent prognostic factor. Conclusion: Our data are the first report of its kind regarding the considerable clinical significance of miR-661 and ATG-4B mRNA in HCC patients. � 2018 2018 Future Medicine Ltd.