Browsing by Author "Maraee A.H."

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Cathepsin D expression in chronic plaque psoriasis: An immunohistochemical study
(2011) Abdou A.G.; Maraee A.H.; Shoeib M.A.E.; Elsammie A.A.A.; Elnaidany N.F.; Pathology Department; Menofiya University; Shebein Elkom; Egypt; Dermatology and Andrology Department; Faculty of Medicine; Menofiya University; Shebein Elkom; Egypt; Clinical Pharmacy Department; Faculty of Pharmacy; MSA University; October City; Egypt
Cathepsins are lysosomal cysteine proteases, which are involved in a variety of physiologic processes such as proenzyme activation, antigen presentation, tissue remodeling, bone matrix resorption, and pathologic processes such as facilitating tumor invasion and modulating the process of programmed cell death. This study aimed to evaluate the pattern of cathepsin D (CD) expression in chronic plaque psoriasis in comparison to normal skin by means of immunohistochemistry. The study included 34 patients presenting with chronic plaque psoriasis and 10 ageand sex-matched normal subjects as control group. Sixty percent of normal skin showed granular positivity for CD confined to basal layer. CD is upregulated in psoaritic lesion with 94.1% positivity making a significant difference between psoriasis and normal skin as regards the percentage and distribution of CD expression, where the latter was predominantly diffuse in psoriatic lesion. The eight cases exposed to PUVA therapy showed reduction of CD positivity to 62.5% with a predominance of mild staining and focal expression compared to pretreatment biopsies. CD may have a role in the pathogenesis of psoriasis in view of its high percentage and diffuse expression in psoriatic epidermis. CD degradative capacity may be responsible for disordered differentiation and scale formation characteristic of psoriasis. Reduction of CD expression may be one of the pathways of PUVA mechanism of action.
Immunohistochemical expression of ezrin in cutaneous basal and squamous cell carcinomas
(2011) Abdou A.G.; Maraee A.H.; El-Sayed E.M.M.; Elnaidany N.F.; Pathology Department; Faculty of Medicine; Menofiya University; Shebein Elkom; 32511; Egypt; Dermatology and Andrology Department; Faculty of Medicine; Menofiya University; Shebein Elkom; 32511; Egypt; Faculty of Pharmacy; MSA University; October city; 12592; Egypt
Ezrin is a member of the ezrin-radixin-moesin family of proteins, which link the actin-containing cytoskeleton to the plasma membrane. Overexpression of ezrin protein is correlated with the metastatic potential in several cancers. Little is known about the distribution of ezrin in normal epidermis and nonmelanoma skin cancer; therefore, in the current study, we examined the immunohistochemical expression of ezrin in normal skin (10 biopsies) and epithelial skin tumors (25 basal cell carcinoma [BCC] and 20 squamous cell carcinoma [SCC]). Ezrin was expressed in epidermis of all normal controls with a prominent membranous pattern compared with 93.3% positivity in malignant cases with a significant higher intensity (assessed by H score) in favor of the latter (P =.002). Cytoplasmic expression of ezrin either alone or associated with membranous expression was both seen in BCC and SCC. The median value of H score in SCC (160) cases was higher than that in BCC (60). H score values of ezrin expression in BCC was significantly higher in tumors arising in sites other than the head and neck (P =.04). In SCC, the intensity of ezrin expression tended to be associated with advanced stage (P =.08). Our study demonstrated the probable tumorigenic role of ezrin in epithelial skin tumor formation. It may enhance local invasion or metastasis of epithelial skin tumors, which necessitates further larger study to clarify. The intensity rather than the pattern of ezrin expression had a more probable impact on the tumor behavior. � 2011 Elsevier Inc. All rights reserved.
Up-regulation of Notch-1 in psoriasis: An immunohistochemical study
(2012) Abdou A.G.; Maraee A.H.; Sharaf A.; Elnaidany N.F.; Pathology Department; Faculty of Medicine; Menofiya University; Shebein Elkom; Egypt; Dermatology and Andrology Department; Faculty of Medicine; Menofiya University; Shebein Elkom; Egypt; Faculty of Pharmacy; MSA University; October City; Egypt
The Notch pathway plays a key role in differentiation, proliferation, and influencing cell fate decision in multiple organisms and tissues including the epidermis and its appendages. The role of Notch-1 in psoriasis has not been widely evaluated; therefore, the current study aimed to evaluate its role in etiopathogenesis of this common skin disease. The current study used immunohistochemical technique to evaluate Notch-1 expression in 35 lesional biopsies of patients having chronic plaque psoriasis in comparison with normal skin biopsies, representing the control group. Notch-1 was expressed in the epidermis of both normal and psoriatic skins; however, the intensity was in favor of psoriatic lesion, and the nuclear form of Notch-1 was more frequently and diffusely seen in psoriasis. Exacerbation of psoriasis as assessed by the Psoriasis Area and Severity Index score was significantly associated with intense (P =.005) and nuclear form of Notch-1 expression (P =.0001). The nuclear form of Notch-1 was also correlated with female sex (P =.043). From this study, up-regulation and not down-regulation of Notch-1 may have a role in pathogenesis of psoriasis. The nuclear form is responsible for the exacerbation of symptoms, and it is the one that may disappear by the effect of psoralen and ultraviolet A radiation (PUVA) therapy. � 2012 Elsevier Inc.