Browsing by Author "M. Fouad, Manal"

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    DEVELOPMENT AND VALIDATION OF CHROMATOGRAPHIC AND SPECTROSCOPIC METHODS FOR ESTIMATION OF REPAGLINIDE AND METFORMIN HCl IN COMBINED DOSAGE FORM
    (Journal of Global Trends in Pharmaceutical Sciences, 2014) M. Fouad, Manal; S. Rashed, Noha
    Two simple, accurate and reproducible methods have been developed for the determination of repaglinide and metformin as bulk or simultaneously in their combined dosage form. The first is a reversed phase HPLC method using C18 column, mobile phase consisting of methanol-0.2% heptane sulphonate sodium (70:30 v/v) at a flow rate of 1ml/min and UV detection at 240 nm. Beer's plot shows good correlations in the concentration range of 1-10 μgml-1 for both drugs. The second method is a derivative spectrophotometry, in which the two drugs were quantified using second derivative responses at 287 nm for repaglinide and at 234 or 252 nm for metformin with a linearity range of 5- 40 or 2-12 μgml-1 for the two drugs, respectively. The proposed methods were validated and successfully applied to the determination of both drugs in their pharmaceutical formulation with mean recoveries of 99.58-100.45 ± 0.386-0.931. The results obtained were statistically compared to those obtained from a reference method and were found to be in good agreement.