Browsing by Author "Hashim A.N."

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Antitumor activity of Cuphea ignea extract against benzo(a)pyrene-induced lung tumorigenesis in Swiss Albino mice
(Taylor and Francis Ltd., 2019) Hassan S.K.; Mousa A.M.; El-Sammad N.M.; Abdel-Halim A.H.; Khalil W.K.B.; Elsayed E.A.; Anwar N.; Linscheid M.W.; Moustafa E.S.; Hashim A.N.; Nawwar M.; Department of Biochemistry; National Research Centre; Dokki; Cairo; Egypt; Department of Cell Biology; National Research Centre; Dokki; Cairo; Egypt; Bioproducts Research Chair; Zoology Department; College of Science; King Saud University; Riyadh; Saudi Arabia; Department of Chemistry of Natural and Microbial Products; National Research Centre; Dokki; Cairo; Egypt; Department of Pathology; National Cancer Institute; Cairo University; Cairo; Egypt; Laboratory of Applied Analytical and Environmental Chemistry; Humboldt-University; Berlin; Germany; October University of Modern Sciences and Arts; 6th October City; Egypt; Department of Phytochemistry and Plant Systematics; National Research Centre; Cairo; Egypt
Lung cancer has one of the highest mortality rates among various types of cancer and is the most frequent cancer in the world. The incidence of lung cancer is increasing rapidly, in parallel with an increased incidence of smoking. Effective chemoprevention may be an alternative strategy to control the incidence of lung cancer. Thus, the objective of current work was to ascertain the possible preventive and therapeutic efficacies of Cuphea ignea extract in a mouse model of lung tumorigenesis and its cytotoxicity toward the A549 human lung cancer cell line. Lung tumorigenesis was induced by the oral administration of benzo(a)pyrene (50 mg/kg b.w.) twice per week to Swiss albino mice for 4 weeks. Benzo(a)pyrene-treated mice were orally administered C. ignea (300 mg/kg body weight, 5 days/week) for 2 weeks before or 9 weeks after the first benzo(a)pyrene dose, for a total of 21 weeks. At the end of the administration period, various parameters were measured in the serum and lung tissues. The results revealed that the oral administration of benzo(a)pyrene resulted in increases in relative lung weight, serum levels of tumor markers (ADA, AHH, and LDH), and the inflammatory marker NF-?B, and a decreased total antioxidant capacity compared with the control. In addition, decreased levels of enzymatic and non-enzymatic antioxidants, with a concomitant increase in lipid peroxidation, metalloproteinases (MMP-2 and MMP-12), and the angiogenic marker VEGF were detected in lung tissues. Moreover, benzo(a)pyrene administration induced the upregulation of PKC?, COX-2, and Bcl-2 expression, with the downregulation of BAX and caspase-3 expression. C. ignea treatment alleviated all alterations in these parameters, which was further confirmed by the histopathological analysis of lung tissues. The findings of the current work provide the first verification of the preventive and therapeutic potentials of C. ignea extract against benzo(a)pyrene-induced lung tumorigenesis in mice. � 2019 The Authors
Antiulcerogenic effect of Cuphea ignea extract against ethanol-induced gastric ulcer in rats
(BioMed Central Ltd., 2019) Mousa A.M.; El-Sammad N.M.; Hassan S.K.; Madboli A.E.N.A.; Hashim A.N.; Moustafa E.S.; Bakry S.M.; Elsayed E.A.; Department of Biochemistry; National Research Centre; Dokki; Cairo; Egypt; Department of Animal Reproduction and Artificial Insemination Research; National Research Centre; Dokki; Cairo; Egypt; Department of Phytochemistry and Plant Systematics; National Research Centre; Dokki; Cairo; Egypt; October University of Modern Sciences and Arts; 6th October City; Egypt; Bioproducts Research Department; Zoology Department; King Saud University; Riyadh; Saudi Arabia; Department of Chemistry of Natural and Microbial Products; National Research Centre; Dokki; Cairo; Egypt
Background: Cuphea ignea is one of the herbal resources belonging to Lythraceae family. Some species of this family have been used traditionally in South and Central America's folk medicine for treating stomach disorders. Therefore, the present study was performed to evaluate the gastropreventive effect of aqueous ethanolic extract of C. ignea aerial parts on ethanol-induced gastric ulcer. Methods: Gastric ulcers were induced in Sprague Dawley rats using one oral dose of absolute ethanol (1.5 mL/rat). The C. ignea aerial parts extract at doses of 250 and 500 mg/kg body weight and ranitidine (a reference drug) at a dose of 30 mg/kg body weight were orally administrated daily for 7 days before ulcer induction. One hour after ethanol administration blood samples were collected and then stomachs of sacrificed rats were subjected to biochemical, macroscopic and microscopic studies. Results: Oral administration of C. ignea extract significantly attenuated gastric ulcer as revealed by significant reduction in the gastric ulcer index and volume of gastric juice while significantly increased preventive percentage, gastric pH value and pepsin activity. Pre-Treatment of C. ignea extract markedly improved the serum level of TNF-?, the gastric MPO activity and NO content. Furthermore, C. ignea pre-Treatment significantly increased the gastric levels of enzymatic and non-enzymatic antioxidants namely CAT, SOD, GSH-Px, and GSH with concomitant reduction in MDA level compared with those in the ethanol group. These results were further supported by histopathological findings which revealed the curing effect of C. ignea on the hemorrhagic shock induced by ethanol toxicity. Conclusions: C. ignea extract showed a potential gastroprotective effect on ethanol-induced gastric ulcer, and its effect may be mediated through suppression of oxidative stress and gastric inflammation. � 2019 The Author(s).
A coumarin with an unusual structure from Cuphea ignea, its cytotoxicity and antioxidant activities
(Govi-Verlag Pharmazeutischer Verlag GmbH, 2018) Moustafa E.S.; Swilam N.F.; Ghanem O.B.; Hashim A.N.; Nawwar M.A.; Lindequist U.; Linscheid M.W.; October University for Modern Sciences and Arts; 6th October City; Egypt; Pharmacognosy Department; Faculty of Pharmacy; British University; Egypt; Under Graduate Research Trainee; Faculty of Pharmacy; German University; Cairo; Egypt; Department of Phytochemistry and Plant Systematics; National Research Center; 14 El Tahrier Str.; Dokki; Cairo; 1361; Egypt; Pharmaceutical Biology; Institute of Pharmacy; Ernst-Moritz-Arndt-University; Greifswald; Germany; Laboratory of Applied Analytical and Environmental Chemistry; Humboldt-University; Berlin; Germany
Phenolic metabolite profiling using two dimensional paper chromatographic analysis (2 DPC) was used for assaying the complex mixture of phenolics of an aqueous ethanol aerial part extract of Cuphea ignea (Lytheraceae). A coumarin with a rare structure, namely, 7-hydroxy 3-methoxy coumarin 5-O-?-glucopyranoside was isolated from the investigated extract. The structure was elucidated by conventional methods and spectral analysis, including one and two dimensional NMR (1D and 2D NMR), as well as by interpretation of the spectra obtained by high resolution electrospray ionization mass technique (HRESIMS). The rare coumarin significantly inhibited reactive oxygen species production with an ED50 value of 6.31�1.64 ?g/ml and 5.78�0.66 ?g/ml as determined by the the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) and the oxygen radical absorption capacity (ORAC) assay respectively. The isolated coumarin presented a cytotoxic activity assessed by using the neutral red assay (NRU) against lung cancer cell line (H23) with IC50 of 40.38�2.75 ?g/ml. � 2018 Govi-Verlag Pharmazeutischer Verlag GmbH. All rights reserved.