Browsing by Author "Hammam M.A."

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Diagnostic and prognostic role of galectin 3 expression in cutaneous melanoma
(2010) Abdou A.G.; Hammam M.A.; Farargy S.E.; Farag A.G.A.; El Shafey E.N.; Farouk S.; Elnaidany N.F.; Pathology Department; Menofiya University; Shebein Elkom; Ahmed Maher Educational Hospital; Cairo; Egypt; Dermatology and Andrology Department; Menofiya University; Shebein Elkom; Ahmed Maher Educational Hospital; Cairo; Egypt; Faculty of Medicine; Menofiya University; Shebein Elkom; Ahmed Maher Educational Hospital; Cairo; Egypt; Clinical Pharmacy Department; Faculty of Pharmacy; MSA University; October City; Egypt
Many of the histopathologic criteria used to diagnose melanoma overlap with atypical but otherwise benign naevi such as dysplastic or Spitz naevi. Galectin-3 is a member of the galectin gene family and is expressed at elevated levels in a variety of neoplastic cell types. The aim of the present study was to investigate the diagnostic value of galectin-3 expression compared with homatropine methyle bromide-45(HMB-45) (one of the established and widely used immunohistochemical melanocytic markers) together with assessment of its prognostic value in melanoma lesions. This study was carried out on 21 cases of melanoma and 20 benign pigmented naevi. Galectin-3 was expressed in all the examined benign and malignant melanocytic lesions. The nucleocytoplasmic pattern of galectin-3 appeared in malignant cases only with 42.86% sensitivity, 100% specificity, and 70.73% accuracy. This pattern tended to be associated with thick melanoma (P = 0.08) and reduced survival (P = 0.22). The intensity of galectin-3 assessed by H-score was significantly of higher values in malignant lesions compared with benign lesions (P < 0.0001). The best cut-off value for discrimination between benign and malignant melanocytic lesions was 295 with 95% sensitivity, 70% specificity, and 83% accuracy. The diagnostic power of galectin-3 in distinguishing between benign and malignant melanocytic lesions relies on the pattern and the intensity of its expression. The nucleocytoplasmic pattern of galectin-3 expression carries greater probability of a malignant phenotype and a poor prognostic impact on patients' outcome. Copyright � 2010 by Lippincott Williams & Wilkins.
Epidemiology of dermatophyte infections among school children in Menoufia Governorate, Egypt
(Blackwell Publishing Ltd, 2018) Farag A.G.A.; Hammam M.A.; Ibrahem R.A.; Mahfouz R.Z.; Elnaidany N.F.; Qutubuddin M.; Tolba R.R.E.; Dermatology; Adrology and STDs Department; Faculty of Medicine; Menoufia University; Shebin El-Kom; Egypt; Department of Public Health and Community Medicine; Faculty of Medicine; Menoufia University; Shebin El-Kom; Egypt; Cleveland Clinic; Lerner College of Medicine; Case Western Reserve University; Cleveland; OH; United States; Clinical Pathology; Faculty of Medicine; Menoufia University; Shebin El-Kom; Egypt; Clinical Pharmacy; Faculty of Pharmacy; MSA University; October City; Egypt; Independent Researcher; Cleveland; OH; United States
Most superficial mycotic infections of human skin are due to dermatophytes. Children are frequently affected due to different predisposing factors, particularly overcrowding in classrooms. This study aimed to estimate the prevalence of dermatophytes infections and their related risk factors among school children in Menoufia Governorate, Egypt. Six public primary and preparatory schools were randomly selected and their pupils (n=3464) were asked to complete a predesigned questionnaire covering both personal data and suspected risk factors for superficial dermatophyte infections. The children were also examined for dermatological diseases. Any suspected lesions were biopsied for mycological examination. The prevalence of clinically suspected dermatophytes infections was 1.41%, whereas the prevalence of culture confirmed cases was 0.98%. The most common clinical type was tinea capitis with a prevalence of 1.01%. Microsporum canis was the only isolated organism from the suspicious lesions with a 69.4% positivity rate. A higher prevalence was observed among boys, low socio-economic pupils and those with a family history of dermatophyte infections. Pet contact and sharing towels and caps among pupils were significant risk factors. Dermatophyte infection is still prevalent among basic school pupils. Fortunately, it is related to preventable risk factors. We recommend regular screening and use of educational health programmes for kids to control it. 2018 Blackwell Verlag GmbH
Macrophage migration inhibitory factor as an incriminating agent in vitiligo
(Sociedade Brasileira de Dermatologia, 2018) Farag A.G.A.; Habib M.S.; Kamh M.E.; Hammam M.A.; Elnaidany N.F.; Dermatology; Andrology and STDs department; Faculty of Medicine; Menoufia UniversityEl Menoufia; Egypt; Medical Biochemistry department; Faculty of Medicine; Menoufia UniversityEl Menoufia; Egypt; Clinical Pharmacy department; Faculty of Pharmacy-MSA University; 6th October City; Egypt
Background: Vitiligo is an autoimmune skin disorder in which the loss of melanocytes is mainly attributed to defective autoimmune mechanisms and, lately, there has been more emphasis on autoinflammatory mediators. Among these is the macrophage migration inhibitory factor, which is involved in many autoimmune skin diseases. However, little is known about the contribution of this factor to vitiligo vulgaris. Objective: To determine the hypothesized role of migration inhibitory factor in vitiligo via estimation of serum migration inhibitory factor levels and migration inhibitory factor mRNA concentrations in patients with vitiligo compared with healthy controls. We also aimed to assess whether there is a relationship between the values of serum migration inhibitory factor and/ or migration inhibitory factor mRNA with disease duration, clinical type and severity in vitiligo patients. Methods: Evaluation of migration inhibitory factor serum level and migration inhibitory factor mRNA expression by ELISA and real-time PCR, respectively, were performed for 50 patients with different degrees of vitiligo severity and compared to 15 age- and gender-matched healthy volunteers as controls. Results: There was a highly significant increase in serum migration inhibitory factor and migration inhibitory factor mRNA levels in vitiligo cases when compared to controls (p<0.001). There was a significant positive correlation between both serum migration inhibitory factor and migration inhibitory factor mRNA concentrations in vitiligo patients, and each of them with duration and severity of vitiligo. In addition, patients with generalized vitiligo have significantly elevated serum migration inhibitory factor and mRNA levels than control subjects. Study limitations: Small number of investigated subjects. Conclusions: Migration inhibitory factor may have an active role in the development of vitiligo, and it may also be a useful index of disease severity. Consequently, migration inhibitory factor may be a new treatment target for vitiligo patients. � 2018 by Anais Brasileiros de Dermatologia.