Browsing by Author "Hamdan, Dalia I"

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Chemical profling and cytotoxic potential of the n-butanol fraction of Tamarix nilotica fowers
(BioMed Central Ltd., 2023-05) Fayed, Marwa A. A; Bakr, Riham O; Yosri, Nermeen; Khalifa, Shaden A. M; El‑Seedi, Hesham R; Hamdan, Dalia I; Refaey, Mohamed S; El‑Seedi, Hesham R; Hamdan, Dalia I; Refaey, Mohamed S
Background Cancer represents one of the biggest healthcare issues confronting humans and one of the big chal‑ lenges for scientists in trials to dig into our nature for new remedies or to develop old ones with fewer side efects. Halophytes are widely distributed worldwide in areas of harsh conditions in dunes, and inland deserts, where, to cope with those conditions they synthesize important secondary metabolites highly valued in the medical feld. Several Tamarix species are halophytic including T.nilotica which is native to Egypt, with a long history in its tradition, found in its papyri and in folk medicine to treat various ailments. Methods LC–LTQ–MS–MS analysis and 1 H-NMR were used to identify the main phytoconstituents in the n- butanol fraction of T.nilotica fowers. The extract was tested in vitro for its cytotoxic efect against breast (MCF-7) and liver cell carcinoma (Huh-7) using SRB assay. Results T.nilotica n-butanol fraction of the fowers was found to be rich in phenolic content, where, LC–LTQ–MS– MS allowed the tentative identifcation of thirty-nine metabolites, based on the exact mass, the observed spectra fragmentation patterns, and the literature data, varying between tannins, phenolic acids, and favonoids. 1 H-NMR confrmed the classes tentatively identifed. The in-vitro evaluation of the n-butanol fraction showed lower activ‑ ity on MCF-7 cell lines with IC50>100 µg/mL, while the higher promising efect was against Huh-7 cell lines with an IC50= 37 µg/mL. Conclusion Our study suggested that T.nilotica fowers’n-butanol fraction is representing a promising cytotoxic can‑ didate against liver cell carcinoma having potential phytoconstituents with variable targets and signaling pathways.
Coriander (Coriandrum sativum L.) essential oil and oil‑loaded nano‑formulations as an anti‑aging potentiality viaTGFβ/SMAD pathway
(Springer nature, 2022-04) Salem, Mohamed A; Manaa, Eman G; Osama, Nada; Aborehab, Nora M; Ragab, Mai F; Haggag, Yusuf A; Ibrahim, Magda T; Hamdan, Dalia I
Aging has become a concern for many people, especially women. Given that high-quality anti-aging products are of high cost; it has imperative to search for other economical sources. Essential oils are frequently used in cosmetics products due to a wide range of biological activities as well as their pleasant odor. The current study aimed to investigate the biochemical efect of the cosmetic potential of selected Apiaceous essential oils, traditionally used for skincare, by evaluating their anti-wrinkle activity. It is worth noting that, coriander essential oil showed the highest collagenase, elastase, tyrosinase, and hyaluronidase inhibitory activities compared to other Apiaceous oils (fennel, anise, and cumin). GC–MS proved that coriander essential oil showed a very high level of oxygenated monoterpenes, with linalool (81.29%) as the most abundant constituent. Intriguingly, coriander oil cream and Coriander Essential Oil-loaded Lipid Nanoparticles (CEOLNs) formulations attenuated in vivo UV-induced skin photoaging that was manifested by signifcantly decreased MDA, COX-2, PGE- 2, MMP-1, JNK, and AP-1 levels. Moreover, these pharmaceutical dosage forms signifcantly increased skin collagen content compared to UV-injured group. Also, coriander essential oil signifcantly increased TGFβ, TGFβII, and SMAD3 protein expression levels compared to UV-injured group. In conclusion, the pharmaceutical dosage forms of coriander oil possess anti-wrinkle activities that could have an auspicious role in amending extrinsic aging.
Identification and analysis of toxic phytochemicals
(Elsevier, 2021-03) Salem, Mohamed A; Serag, Ahmed; El-Seedi, Hesham R.; Hamdan, Dalia I; Ezzat, Shahira M; Zayed, Ahmed
Plants and plant products are used in food and medicine for thousands of years ago. It was thought that natural means safe, but this is not always the case as many plants or plant constituents are toxic or even poisonous especially those containing cardiac, cyanogenic, and thio-glycosides and certain types of alkaloids. Toxic natural products may cause hepatotoxicity, neurotoxicity, gastrointestinal toxicity, renal toxicity, and cytotoxicity. Analysis of toxic plants and plant constituents is facing a great challenge because of their different chemical and physicochemical properties. Novel extraction techniques have been proposed such as ultrasonic-assisted extraction, microwave-assisted extraction, supercritical fluid extraction, and accelerated solvent extraction. In this chapter, we will have an overview of some of the toxic plants and phytochemicals as well as the methods for their qualitative and quantitative analysis.
Preparation and in vivo evaluation of nano sized cubosomal dispersion loaded with Ruta graveolens extracts as a novel approach to reduce asthma-mediated lung inflammation
(King Saud University, 2024-02) Alyami, Mohammad H; Hamdan, Dalia I; Khalil, Heba M.A; Orabi, Mohamed A.A; Aborehab, Nora M; Osama, Nada; Abdelhafez, Mai M; Al-Mahallawi, Abdulaziz Mohsen; Alyami, Hamad S
Asthma is a chronic disease affecting people of all ages. Asthma medications are associated with adverse effects restricting their long-term usage, demanding newer alternative therapies. This study aimed to investigate the anti-asthmatic properties of Ruta graveolens extract and its prepared nano-cubosomal dispersion (Ruta-ND). Firstly, the R. graveolens methanolic extract exhibited higher anti-inflammatory activity on Lipopolysaccharide (LPS)-activated BEAS-2B cells. To ensure best bioavailability and hence best cellular uptake, R. graveolens extract was loaded in nano-cubosomal dispersion (ND). Then, the anti-asthmatic effects of Ruta extract and ND were simultaneously evaluated in rats’ model with ovalbumin-induced allergic asthma. R. graveolens extract and RutaND subsided asthma score and improved lung function by restoring FEV1/FVC ratio to the expected values in control rats. Also, it showed strong antioxidant and anti-inflammatory activities manifested by lowering levels of malondialdehyde (MDA), IL-4, IL-7, TGF-β, and Ig-E, and increasing levels of superoxide dismutase (SOD) and INF-γ in bronchoalveolar lavage fluid. Our research findings also indicate autophagy induction and apoptosis inhibition by Ruta extract and Ruta-ND. Finally, the HPLC MS/MS phytochemical profiling of R. graveolens extract evident production of various alkaloids, flavonoids, coumarins, and other phenolics with reported pharmacological properties corresponding to/emphasize our study findings. In conclusion, R. graveolens exhibited promise in managing Ova-induced allergic asthma and could be developed as an alternative antiallergic asthma drug.