Browsing by Author "Gad, Sameh S"

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Hepatoprotective Effect of Silver Nanoparticles at Two Different Particle Sizes: Comparative Study with and without Silymarin
(Multidisciplinary Digital Publishing Institute (MDPI), 2022-06-30) Elfaky, Mahmoud A; Sirwi, Alaa; Ismail, Sameh H; Awad, Heba H; Gad, Sameh S
Silver nanoparticles have been used for numerous therapeutic purposes because of their increased biodegradability and bioavailability, yet their toxicity remains questionable as they are known to interact easily with biological systems because of their small size. This study aimed to investigate and compare the effect of silver nanoparticles’ particle size in terms of their potential hazard, as well as their potential protective effect in an LPS-induced hepatotoxicity model. Liver slices were obtained from Sprague Dawley adult male rats, and the thickness of the slices was optimized to 150 µm. Under regulated physiological circumstances, freshly cut liver slices were divided into six different groups; GP1: normal, GP2: LPS (control), GP3: LPS + AgNpL (positive control), GP4: LPS + silymarin (standard treatment), GP5: LPS + AgNpS + silymarin (treatment I), GP6: LPS + AgNpL + silymarin (treatment II). After 24 h of incubation, the plates were gently removed, and the supernatant and tissue homogenate were all collected and then subjected to the following biochemical parameters: Cox2, NO, IL-6, and TNF-α. The LPS elicited marked hepatic tissue injury manifested by elevated cytokines and proinflammatory markers. Both small silver nanoparticles and large silver nanoparticles efficiently attenuated LPS hepatotoxicity, mainly via preserving the cytokines’ level and diminishing the inflammatory pathways. In conclusion, large silver nanoparticles exhibited effective hepatoprotective capabilities over small silver nanoparticles.
Nanoparticles: A New Approach for treatment of bacterial and viral hepatic infections via modulating oxidative stress and DNA fragmentation
(Academic Press Inc., 2022-06) Gad, Sameh S; Abdelrahim, Dina S; Ismail, Sameh H; Ibrahim, Sherine M
Background: Nanoparticles are recently playing a potential role in improving drug uptake and the treatment of diseases. A variety of nanoparticles, such as selenium nanoparticles (SeNPs) and Silver nanoparticles (AgNPs) have been used as drug carriers in various ways for treatment of cancers and liver diseases. Our aim in this study is to investigate the ability of AgNPs and SeNPs to target and treat the viral and bacterial infection of liver in rats and cell lines. Methods: For assessment of antioxidant activity of silver nanoparticles, six adult male albino rats were included in this study, liver slices were taken and assigned to 6 groups. Markers of hepatic functions, oxidative stress and inflammation in liver slices are carried out. While for assessment of antiviral activity of SeNPs, HBV-replicating human cell line HepG2 and normal human cell lines were used, hepatic and inflammatory alterations are determined through quantitative polymerase chain reaction (PCR) and comet assay techniques. Results: The effect of Ag-NPs on interleukin-6 (IL-6) and tumor necrosis factor (TNF-α) levels were reduced in different treated groups with Ag-NPs compared with the control and diseased groups. On the other hand, SeNPs revealed significant alterations in the inflammatory markers as well as DNA damage in the treated HBV- human cell line HepG2 compared to the diseased ones. Conclusion: Silver nanoparticles have the ability for producing various hepatic alterations and can inhibit the proliferation of hepatic stellate cells (HSCs) in a dose and size dependent manner. On the other hand, SeNPs showed excellent selectivity towards viral cells in the HepG2 cell lines. Both Ag-NPs and SeNPs might be a promising drug design for treating viral and bacterial liver diseases.
Nanotechnology applications for treatment of hepatic infections via modulating Hepatic histopathological and DNA alterations
(Academic Press Inc., 2022-06-17) Gad, Sameh S; Abdelrahim, Dina S; Ismail, Sameh H; Ibrahim, Sherine M
Background: Nanoparticles are recently playing a potential role in improving drug uptake and the treatment of diseases. A variety of nanoparticles, such as selenium nanoparticles (SeNPs) and Silver nanoparticles (AgNPs) have been used as drug carriers in various ways for treatment of cancers and liver diseases. Our aim in this study is to investigate the ability of AgNPs and SeNPs to target and treat the viral and bacterial infection of liver in rats and cell lines. Methods: For assessment of antioxidant activity of silver nanoparticles, six adult male albino rats were included in this study, liver slices were taken and assigned to 6 groups. Markers of hepatic functions, oxidative stress and inflammation in liver slices are carried out. While for assessment of antiviral activity of SeNPs, HBV-replicating human cell line HepG2 and normal human cell lines were used, hepatic and inflammatory alterations are determined through quantitative polymerase chain reaction (PCR) and comet assay techniques. Results: The effect of Ag-NPs on interleukin-6 (IL-6) and tumor necrosis factor (TNF-α) levels were reduced in different treated groups with Ag-NPs compared with the control and diseased groups. On the other hand, SeNPs revealed significant alterations in the inflammatory markers as well as DNA damage in the treated HBV- human cell line HepG2 compared to the diseased ones. Conclusion: Silver nanoparticles have the ability for producing various hepatic alterations and can inhibit the proliferation of hepatic stellate cells (HSCs) in a dose and size dependent manner. On the other hand, SeNPs showed excellent selectivity towards viral cells in the HepG2 cell lines. Both Ag-NPs and SeNPs might be a promising drug design for treating viral and bacterial liver diseases.
A new arsenal of polyphenols to make Parkinson's disease extinct: HPLC-MS/MS profiling, very interesting MAO-B inhibitory activity and antioxidant activity of Otostegia fruticosa
(Taylor and Francis, 16/02/2022) Al-Madhagy, Somaia A; Gad, Sameh S; Mostafa, Eman S; Angelonie, Simone; Saad, Muhammed A; Sabry, Omar M; Caprioli, Giovani; El-Hawary, Seham S
Fifteen compounds belong to phenolic acids, derivatives of phenolic acids, iridoids, xanthones and flavonoids were characterized in the methanolic extract of Otostegia fruticosa leaves using HPLC-MS/MS. Extract has been also investigated for its MAO-B inhibitory activity, antioxidant activity, total phenolic and total flavonoid content. The extract exhibited interesting MAO-B inhibitory activity (IC50; 2.24 ± 0.08) compared to the reference compound selegiline (0.55 ± 0.02 µg/mL). It also showed a potent antioxidant activity proven in both DPPH and ORAC assay methods. The extract showed an IC50 of 3.64 ± 1.22 µg/mL in the DPPH test which was significantly lower than that of the standard ascorbic acid which attained an IC50 of 18.3 ± 1.41 µg/mL. Moreover, in the oxygen radical absorbance capacity assay (ORAC) the extract showed a decline in the IC50 to 3.48 ± 1.16 µg/mL as compared to the standard Trolox which exhibited an IC50 of 27.0 ± 13.41.
A new firewall in the fight against breast cancer: in-vitro and in-silico studies correlating chemistry to apoptotic activity of Otostegia fruticosa
(Taylor and Francis Ltd., 2022-10) Al-Madhagy, Somaia. A; Gad, Sameh S; Mostafa, Eman S; Angelonie, Simone; Saad, Muhammed A; Sabry, Omar M; Caprioli, Giovanni; El-Hawary, Seham S
Breast cancer is the most devastating disease for women. There is a great demand for new sources to treat this disease. Medicinal plants are an indispensable source of bioactive compounds with wide range of pharmacological activities. In-vitro cytotoxic activity of Otostegia fruticosa methanolic extract against human breast cancer was studied using MCF-7 cell line. The extract showed mildly potent activity (IC50 ¼ 51 ± 9.836 mg/mL) in comparison to the standard anticancer doxorubicin (IC50 ¼ 7.467 ± 1.05 mg/mL). Potential compounds responsible for activity have been identified using Molecular Operating Environment (MOE) module on the major compounds detected by HPLC-MS/MS technique against estrogen alpha receptor (ERaþ: PDB ID 2JF9). 3,5-di-O-dicaffeoyl- quinic acid, hyperoside and rutin showed similar binding and antagonistic interaction with the estrogen alpha receptor as tam- oxifen in several poses. The retrieved results confirm that we can add this plant to a powerful arsenal that combats this insidious disease.
Pharmacological Study on Activity of Silymarin and/or Zinc Oxide Nanoparticles against Hepatic Dysfunction Using Cell Line HepG2
(MEDWIN PUBLISHERS, 07/11/2019) Gad, Sameh S
Introduction: The liver is the primary organ for metabolism. The increase of use in nanoparticles nowadays is scary to such an important organ unless it is fully investigated. Therefore, our aim is evaluating the hepatoprotective effect of silymarin in alone and in combination with zinc oxide nanoparticles Zno NPs against hepatic dysfunction induced by methotrexate (MTX) on human liver cells (HepG2). Methotrexate is an anti-metabolite that is used in treating autoimmune illness and cancer but it induces liver toxicity so that its application is limited. Methods and Results: In our study, we investigated the hepatoprotective effect of Zno NPs, and silymarin against HepG2 cells induced by (MTX). Doses were determined according to determination of the IC50 for each compound, dose of induction used was 75 μg/ml of methotrexate. Results showed that Zno NPs with silymarin exert a distinct effect on cells viability by causing a great hepatoprotection for Hep G cells. We used different doses for both silymarin and Zno NPs. Doses used for silymarin were (100, 75 and 50 μg/ml), and doses of Zno NPs were (20, 10 and 5 μg/ml), but actually the most hepatoprotective doses exerted by Zno NPs and silymarin were 10 and 50 μg/ml which achieved about 97% cell viability. Conclusion: Overall, our data demonstrated that Zno NPs in combination with silymarin cause a hepatoprotection for Hep G2 cells pretreated with methotrexate to cause liver cytotoxicity. This study provides guidance for development of treatment of liver dysfunction using this combination of Zno NPs and silymarin. in addition, this study suggests that further investigation required for better understanding of nanoparticles use
Selenium and silver nanoparticles: A new approach for treatment of bacterial and viral hepatic infections via modulating oxidative stress and DNA fragmentation
(Wiely, 09/12/2021) Gad, Sameh S; Abdelrahim, Dina S; Ismail, Sameh H; Ibrahim, Sherine M
Nanoparticles are recently playing a potential role in improving drug uptake and the treatment of diseases. A variety of nanoparticles, such as selenium nanoparticles (SeNPs) and silver nanoparticles (AgNPs) have been used as drug carriers in various ways for treatment of cancers and liver diseases. Our aim in this study is to investigate the ability of AgNPs and SeNPs to target and treat the viral and bacterial infection of the liver in rats and cell lines. For assessment of antioxidant activity of AgNPs in rats with induced liver bacterial infection, six adult male albino rats were included in this study, liver slices were taken and assigned to 6 groups. Markers of hepatic functions, oxidative stress, and inflammation in liver slices are carried out. Although for assessment of antiviral activity of SeNPs, hepatitis B virus transfected (HBV)‐replicating human cell line HepG2 and normal hepatocyte cells were used, hepatic and inflammatory alterations are determined through quantitative polymerase chain reaction and comet assay techniques. The effect of AgNPs on interleukin‐6 and tumor necrosis factor levels were reduced in different treated groups with AgNPs compared with the control and diseased groups. On the other hand, SeNPs revealed significant alterations in the inflammatory markers as well as DNA damage in the treated HBV‐human cell line HepG2 compared to the diseased ones. AgNPs have the ability for producing various hepatic alterations and can inhibit the proliferation of hepatic stellate cells (HSCs) in a dose and size‐dependent manner. On the other hand, SeNPs showed excellent selectivity towards viral cells in the HepG2 cell lines. Both AgNPs and SeNPs might be promising drug designs for treating viral and bacterial liver diseases.
Therapeutic Potential of Pavetta L. Genus: An Updated Review of Its Traditional Uses, Phytochemistry, Pharmacology and Toxicological Aspects
(NIDOC (Nat.Inform.Document.Centre), 2022-10) Al-Madhagy, Somaia. A; El-Hawary, Seham S; Sabry, Omar M; Gad, Sameh S; Mostafa, Eman S
Pavetta L. genus is one of the largest genera in Rubiaceae family. Although of the wide distribution and the variety of traditional uses of it plants, research covered only limited species of Pavetta L. genus. The purpose of this review is to provide an in-depth understanding of traditional uses, phytochemical, pharmacological, and toxicological characteristics of these plants, which may lead to new insights into new therapeutic benefits of them. To achieve that, literatures without time limitation was used to gather information about all features of Pavetta L. genus including its botanical description and geographical distribution.
Therapeutic Potential of Pavetta L. Genus: An Updated Review of Its Traditional Uses, Phytochemistry, Pharmacology and Toxicological Aspects
(NIDOC (Nat.Inform.Document.Centre), 2023-02) Al-Madhagy, Somaia. A; El-Hawary, Seham S; Sabry, Omar M; Gad, Sameh S; Mostafa, Eman S
Pavetta L. genus is one of the largest genera in Rubiaceae family. Although of the wide distribution and the variety of traditional uses of it plants, research covered only limited species of Pavetta L. genus. The purpose of this review is to provide an in-depth understanding of traditional uses, phytochemical, pharmacological, and toxicological characteristics of these plants, which may lead to new insights into new therapeutic benefits of them. To achieve that, literatures without time limitation was used to gather information about all features of Pavetta L. genus including its botanical description and geographical distribution. :
A Unique Acylated Flavonol Glycoside from Prunus persica (L.) var. Florida Prince: A New Solid Lipid Nanoparticle Cosmeceutical Formulation for Skincare
(MDPI, 03/12/2021) Mostafa, Eman S; Maher, Ahmed; Mostafa, Dalia A; Gad, Sameh S; Nawwar, Mahmoud A.M; Swilam, Noha
MDPI Open Access Journals zoom_out_map search menu Journals Antioxidants Volume 10 Issue 3 10.3390/antiox10030436 antioxidants-logo Submit to this Journal Review for this Journal Edit a Special Issue ► Article Menu Open AccessArticle A Unique Acylated Flavonol Glycoside from Prunus persica (L.) var. Florida Prince: A New Solid Lipid Nanoparticle Cosmeceutical Formulation for Skincare by Eman S. Mostafa 1,*,Ahmed Maher 2,*OrcID,Dalia A. Mostafa 3OrcID,Sameh S. Gad 4OrcID,Mahmoud A.M. Nawwar 5 andNoha Swilam 6OrcID 1 Department of Pharmacognosy, Faculty of Pharmacy, October University of Modern Sciences and Arts (MSA), Giza 12451, Egypt 2 Department of Biochemistry, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), Giza 12451, Egypt 3 Department of Pharmaceutics, Faculty of Pharmacy, October University of Modern Sciences and Arts (MSA), Giza 12451, Egypt 4 Department of Pharmacology, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), Giza 12451, Egypt 5 National Research Centre, Department of Phytochemistry, Dokki, Cairo 12622, Egypt 6 Department of Pharmacognosy, Faculty of Pharmacy, The British University in Egypt (BUE), Cairo 11837, Egypt * Authors to whom correspondence should be addressed. Academic Editors: Alfredo Aires and Yong Chool Boo Antioxidants 2021, 10(3), 436; https://doi.org/10.3390/antiox10030436 Received: 24 February 2021 / Revised: 6 March 2021 / Accepted: 8 March 2021 / Published: 12 March 2021 (This article belongs to the Special Issue Phenolics as Antioxidant Agents) View Full-Text Download PDF Browse Figures Citation Export Abstract Polyphenols are known dietary antioxidants. They have recently attracted considerable interest in uses to prevent skin aging and hyperpigmentation resulting from solar UV-irradiation. Prunus persica (L.) leaves are considered by-products and were reported to have a remarkable antioxidant activity due to their high content of polyphenols. This study aimed at the development of a cosmeceutical anti-aging and skin whitening cream preparation using ethanol leaves extract of Prunus persica (L.) (PPEE) loaded in solid lipid nanoparticles (SLNs) to enhance the skin delivery. Chemical investigation of PPEE showed significantly high total phenolic and flavonoids content with notable antioxidant activities (DPPH, ABTS, and β-carotene assays). A unique acylated kaempferol glycoside with a rare structure, kaempferol 3-O-β-4C1-(6″-O-3,4-dihydroxyphenylacetyl glucopyranoside) (KDPAG) was isolated for the first time and its structure fully elucidated. It represents the first example of acylation with 3,4-dihydroxyphenyl acetic acid in flavonoid chemistry. The in-vitro cytotoxicity studies against a human keratinocytes cell line revealed the non-toxicity of PPEE and PPEE-SLNs. Moreover, PPEE, PPEE-SLNs, and KDPAG showed good anti-elastase activity, comparable to that of N-(Methoxysuccinyl)-Ala-Ala-Pro-Val-chloromethyl ketone. Besides, PPEE-SLNs and KDPAG showed significantly (p < 0.001) higher anti-collagenase and anti-tyrosinase activities in comparison to EDTA and kojic acid, respectively. Different PPEE-SLNs cream formulae (2% and 5%) were evaluated for possible anti-wrinkle activity against UV-induced photoaging in a mouse model using a wrinkle scoring method and were shown to offer a highly significant protective effect against UV, as evidenced by tissue biomarkers (SOD) and histopathological studies. Thus, the current study demonstrates that Prunus persica leaf by-products provide an interesting, valuable resource for natural cosmetic ingredients. This provides related data for further studying the potential safe use of PPEE-SLNs in topical anti-aging cosmetic formulations with enhanced skin permeation properties