Browsing by Author "Elshishtawy, Hisham"

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    Antibacterial Effect of Pre-constructed 3D Bone Scaffolds before and after Modification with Propolis
    (Scientific Foundation SPIROSKI, 02/01/2022) Hesham, Mai; Elshishtawy, Hisham; El Kady, Sherihan; Wahied, Dina
    AIM: This study was to determine and compare the antibacterial activity of different scaffold materials before and after their modification with ethanolic extract of Egyptian propolis ethanolic extract of propolis (EEP). SETTINGS AND DESIGN: Preparation of the dry mass of propolis, preparation of EEP, preparation of the scaffolds, and antibacterial activity testing. MATERIALS AND METHODS: Four bacterial strains were used to determine the antibacterial activity of two different scaffold materials before and after their modification with EEP (15% and 25% by weight). RESULTS: Tricalcium phosphate + gelatin binder modified by 25% EEP exhibited the highest antibacterial activity against Escherichia coli. While, tricalcium phosphate + (alginate and cellulose nanowhiskers) binder modified by 25% EEP demonstrated the highest antibacterial activity Staphylococcus aureus, Streptococcus mutans, and Lactobacillus casei. CONCLUSIONS: It can be concluded that EEP had a significant effect on the antibacterial activity of both scaffold materials; the antibacterial activity was higher against Gram-positive bacteria.
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    COMPARATIVE EVALUATION OF THE ANTIBACTERIAL ACTIVITY OF GLASS IONOMER RESTORATION INCORPORATED WITH DIFFERENT METAL OXIDES NANOPARTICLES ON ORAL STREPTOCOCCUS MUTANS
    (EDJ, 2019-10) Ezzeldin, Naglaa; Elshishtawy, Hisham; El-Borady, Ola; Mohamed, Mona
    Objective: The objective of this study is to compare and evaluate the antibacterial activity of modified conventional Glass ionomer restoration (GI) with zinc oxide (ZnO-NPs) and cerium oxide Nanoparticles (CeO2-NPs) on the oral Streptococcus mutans (SM). Materials and Methods: one hundred and five disc-shaped specimens were divided into seven groups; GI, GI incorporated with three, five and seven percent ZnO-NPs and CeO2-NPs. Antibacterial activity was assessed by counting of colony forming unit (CFU) of SM after direct contact test and 96 hours biofilm evaluation. Fourier transform infrared spectroscopy (FTIR) was used to illustrate interaction between different concentrations of Nanoparticles incorporated with GI. Results: Direct contact test showed a significant reduction of CFU of GI incorporated with seven percent ZnO-NPs in relation to GI (P value 0.007). Biofilm evaluation showed significant reduction of CFU of GI incorporated with three, five and seven percent ZnO-NPs and five and seven percent CeO2-NPs (P value 0.000). FTIR showed Peaks at 1405 and 1635 cm–1 related to symmetric and asymmetric vibrations of COO−, those peaks were broader and red shifted in GI incorporated with ZnO-NPs and CeO-NPs. Conclusion: Incorporation of conventional GI with ZnO-NPs and CeO2-NPs enhance its antibacterial activity against oral SM.