Browsing by Author "El-Sharabasy, Sherif"

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Effect of Amino Acids on the Growth and Production of Steroids in Date Palm Using Tissue Culture Technique
(Researcher, 2012) El-Sharabasy, Sherif; Ahmed Farag, Mai; El-Emerym, GAE; Safwat, Gehan; Diab, Ayman A
The present investigation studied the effect of amino acids (Glutamine, Spermidine and Asparagine) with different concentration (50, 250.500 mg/l) used as precursors to produce secondary metabolites (steroids) and growth development during different stages (callus, embryoids and shooting) of date palm (Malakaby cv.). In Embryogenic callus stage,callus volume was the highest (4.00) when treated with any of the three amino acids, 50 mg/l of Glutamine or Asparagine showed no effect compared to the control giving the lowest callus volume (3.00). Total steroids in callus tissues clearly showed that using Glutamine 250 mg/l in medium gave the highest steroid content 0.662 mg/g and percentage (336% of control), while the lowest (0.111mg/g) was found with Asparagine (500mg/l) and 56.35% of control. Glutamine at 250 mg/l resulted in the highest weight of embryos (2.100 gm). As well as, 500 mg/l Spermidine seemed to be the best amino acid used in order to stimulate steroid biosynthesis resulting in 202.1% of control (0.782 mg/g). In shooting stage, according to the number of shoots, the highest number of shoots (2.33) was achieved with Glutamine and Spermidine at 500 mg/l. In shoot weight, the highest weight (7.267 gm) was achieved by using 500mg/l Glutamine , as to steroid biosynthesis in shooting stage, the best result obtained, were by using Glutamine at 500 mg/l which gave highest steroid biosynthesis (0.534mg/g), 206.0% of contro
The effect of natural antioxidant(s) on date palm (Phoenix dactylifera L.) in vitro
(Academic Journals (Kenya), 2014) Safwat, Gehan; El-Sharabasy, Sherif; El-Banna, A El-Moneam; Khede Zardah, Saleh; Hamido, Nashwa
Date palm (Phoenix dactylifera L.) is one of the most valuable economic resources in the Middle East and North Africa that grow on monocotyledonous trees. To increase crop yield of palm trees, in vitro micro-propagation has become an attractive alternative for large-scale production of date palm. A problem that frequently damages tissues in the early micro-propagation is the brown color that advances in the callus culture due to the creation of quinones. Quinones seize plant cellular developments which lead to cellular decay. This study advocates the use of antioxidant factors found in spinach, kale and strawberries within various concentrations (50, 150 and 300 mg/L) with respect to the medium culture, in an attempt to reduce the level of total phenol and browning which occurs, and also to improve growth and development in different in vitro stages of date palm (P. dactylifera L.). The results indicate that better growth value of callus was achieved using 150 mg/L of kale concentration; allowing the total phenol level to be reduced to 0.9237 mg/g D.W, presenting a significant growth value in comparison to the other treatments in the embryonic callus stage. In the date palm’s somatic embryogenesis stage, the results show that the use of 50 mg/L of spinach, 50 mg/L of kale, 150 mg/L of strawberries, achieved a high number of somatic embryos and the total phenol level was reduced to 0.6167 mg/g D.W. Results from date palm shoot proliferation shows that high numbers of shoot (16.3) was achieved using 50 to 300 mg/L of kale; however, total phenol level was reduced to 0.04567 at 150 mg/L of spinach concentration. The fluctuation of reducing total phenol level in date palm was recorded when the explants were grown on medium supplemented with 50 mg/L of kale concentration.
Influence of Nitrogen Source for the Improvement of Shoots and Roots on in vitro Strawberry (Fragaria ananassa DUCH)
(Agricultural Chemistry &Environmental Protection Society, 2014) S. Hassan, Osama; El-Sharabasy, Sherif; M. Aziz, Marina
Using Some Micro-elements to Improve Shoot and Root Induction of Date Palm (Phoenix dactylifera L.)
(Agricultural Chemistry & Environmental Protection society, 2013) Hassan, Osama; Hammad, Maged; El-Sharabasy, Sherif; Safwat, Gehan