Browsing by Author "El-Khadragy M.F."

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Ceratonia siliqua pod extract ameliorates Schistosoma mansoni-induced liver fibrosis and oxidative stress
(BioMed Central Ltd., 2016) Al-Olayan E.M.; El-Khadragy M.F.; Alajmi R.A.; Othman M.S.; Bauomy A.A.; Ibrahim S.R.; Abdel Moneim A.E.; King Saud University; Department of Zoology; Faculty of Science; Riyadh; Saudi Arabia; University of Helwan; Department of Zoology and Entomology; Faculty of Science; Cairo; Egypt; University of Hail; Faculty of Preparatory year; Hail; Saudi Arabia; October University for Modern Science and Arts (MSA); Faculty of Biotechnology; Giza; Egypt; Qassim University; Laboratory Sciences Department; College of Science and Arts; Al-Rass; Saudi Arabia; National Organization for Drug Control and Research (NODCAR); Molecular Drug Evaluation Department; Giza; Egypt
Background: Schistosomiasis is a prevalent parasitic disease found predominantly in tropical and sub-tropical areas of the developing world, with the second highest socioeconomic and public health burden despite strenuous control efforts. In the present study, we aimed to investigate the ameliorative effects of Ceratonia siliqua pod extract (CPE) on liver fibrosis and oxidative stress in mice infected with Schistosoma mansoni. Methods: The schistosomal hepatopathologic mouse model was established by tail immersion with schistosomal cercaria. The extract was given daily for 10 days beginning 42 days post-infection. Liver samples were obtained from mice sacrificed 9 weeks after infection. Liver histopathological changes were observed with hematoxylin-eosin and Masson trichrome staining. Results: Typical schistosomal hepatopathologic changes were induced in the untreated mice. However, the oral administration of CPE was effective in reducing worm number and the egg load in the liver. This treatment also decreased granuloma size and collagen deposition by inhibiting tissue inhibitor of metalloproteinases-2 (TIMP-2) expression. Schistosomal infection induced oxidative stress by increasing lipid peroxidation (LPO) and nitrite/nitrate (nitric oxide; NO) production along with concomitant decreases in glutathione (GSH) and various antioxidant enzymes, including superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase. However, treatment of mice with CPE at 300 or 600 mg/kg inhibited LPO and NO production, increased GSH content, and restored the activities of the antioxidant enzymes compared with untreated infected mice. Furthermore, treatment with CPE inhibited apoptosis, as indicated by the reduced Bax expression in hepatic tissue. Conclusion: These data indicated that extracts from Ceratonia siliqua pods may play an important role in combating schistosomal hepatopathology and may inhibit granuloma formation and liver fibrosis through down-regulation of TIMP-2 expression. 2016 The Author(s).
Erratum to: Ceratonia siliqua pod extract ameliorates Schistosoma mansoni-induced liver fibrosis and oxidative stress [BMC Complementary and Alternative Medicine, 16, (2016) (434)] DOI: 10.1186/s12906-016-1389-1
(BioMed Central Ltd., 2017) Al-Olayan E.M.; El-Khadragy M.F.; Alajmi R.A.; Othman M.S.; Bauomy A.A.; Ibrahim S.R.; Moneim A.E.A.; King Saud University; Chair vaccines research of infectious diseases; Faculty of Science; Riyadh; Saudi Arabia; King Saud University; Department of Zoology; Faculty of Science; Riyadh; Saudi Arabia; University of Helwan; Department of Zoology and Entomology; Faculty of Science; Cairo; Egypt; University of Hail; Faculty of Preparatory year; Hail; Saudi Arabia; October University for Modern Science and Arts (MSA); Faculty of Biotechnology; Giza; Egypt; Qassim University; Laboratory Sciences Dept.; College of Science and Arts; Al-Rass; Saudi Arabia; Molecular Drug Evaluation Department; National Organization for Drug Control and Research (NODCAR); Giza; Egypt
Following publication of the original article [1] it wasbrought to our attention that the affiliations for thisarticle had been incorrectly presented. Please notethat the correct affiliations should be as follows:Ebtesam M. Al-Olayan 1,2 , Manal F. El-Khadragy 1,2,3, Reem A. Alajmi 1 , Mohamed S. Othman 4,5 , Amira A.Bauomy 3,6 , Shaimaa R. Ibrahim 7 and Ahmed E. Abde l Moneim 3* 1 Chair vaccines research of infectious diseases, Facultyof Science, King Saud University, Riyadh, and KSA 2 Department of Zoology, Faculty of Science, King SaudUniversity, Riyadh, KSA 3 Department of Zoology and Entomology, Faculty ofScience, University of Helwan, Cairo, Egypt 4 Faculty of Preparatory year, University of Hail, Hail,KSA 5 Faculty of Biotechnology, October University forModern Science and Arts (MSA), Giza, Egypt 6 Laboratory Sciences Dept., College of Science andArts, Qassim University, Al-Rass, KSA 7 Molecular Drug Evaluation Department, NationalOrganization for Drug Control and Research (NODCAR),Giza, EgyptPlease also note that the Acknowledgements sectionshould be updated to the following: "The authors extendtheir appreciation to the Deanship of Scientific Researchat King Saud University for funding the work throughthe research group project No. RGPVPP-074". � The Author(s).
The potential protective effect of physalis peruviana L. against carbon tetrachloride-induced hepatotoxicity in rats is mediated by suppression of oxidative stress and downregulation of MMP-9 expression
(Elsevier Ltd., 2014) Al-Olayan E.M.; El-Khadragy M.F.; Aref A.M.; Othman M.S.; Kassab R.B.; Abdel Moneim A.E.; Zoology Department; Faculty of Science; King Saud University; Riyadh 11451; Saudi Arabia; Zoology and Entomology Department; Faculty of Science; Helwan University; Cairo 11795; Egypt; Biological Science Department; Faculty of Dentistry; Modern Sciences and Arts (MSA) University; Giza 12111; Egypt; Biochemistry and Molecular Biology Department; Faculty of Biotechnology; Modern Sciences and Arts (MSA) University; Giza 12111; Egypt; Experimental Biology Department; Faculty of Science; Masaryk University; 62500 Brno; Czech Republic; Biochemistry and Molecular Biology Department; Asturias Institute of Biotechnology; University of Oviedo; 33006 Oviedo; Spain
The active constituent profile in Cape gooseberry (Physalis peruviana L.) juice was determined by GC-MS. Quercetin and kaempferol were active components in the juice. In this study we have evaluated its potential protective effect on hepatic injury and fibrosis induced by carbon tetrachloride (CCl4). Twenty-eight rats divided into 4 groups: Group I served as control group, and Group II received weekly i.p. injection of 2 mL CCl4/kg bwt for 12 weeks. Group III were supplemented with Physalis juice via the drinking water. The animals of Group IV received Physalis juice as Group III and also were intraperitoneally injected weekly with 2 mL CCl4/kg bwt for 12 weeks. Hepatoprotective effect was evaluated by improvement in liver enzymes serum levels, reduction in collagen areas, downregulation in expression of the fibrotic marker MMP-9, reduction in the peroxidative marker malonaldehyde and the inflammatory marker nitric oxide, and restoration of the activity of antioxidant enzymatic and nonenzymatic systems, namely, glutathione content, superoxide dismutase, catalase, glutathione-S-transferase, glutathione peroxidase, and glutathione reductase activities. The results show that the potential hepatoprotective effects of Physalis peruviana may be due to physalis acts by promotion of processes that restore hepatolobular architecture and through the inhibition of oxidative stress pathway. � 2014 Ebtisam M. Al-Olayan et al.