Browsing by Author "El Bishbishy M.H."

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Chemometric discrimination of three Pistacia species via their metabolic profiling and their possible in vitro effects on memory functions
(CIC Edizioni Internazionali s.r.l., 2020) El Bishbishy M.H.; Gad H.A.; Aborehab N.M.; Pharmacognosy Department; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA); Giza; Egypt; Pharmacognosy Department; Faculty of Pharmacy; Ain Shams University; Cairo; Egypt; Biochemistry Department; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA); Giza; Egypt
Alzheimer's disease (AD) is the most widespread neurodegenerative disease; there are around ten million new cases of Alzheimer yearly worldwide especially in middle or low-income countries. Pistacia is a genus of flowering plants including the well-known, economically important P. chinensis Bunge, P. lentiscus L. and P. khinjuk. In this study, the metabolic profiling of Pistacia leaves extracts was achieved via UHPLC-ESI-MS analysis and GC�MS analysis employing chemometric analysis for their discrimination. In addition, the methanolic extracts of different Pistacia species were assessed for their anti-cholinesterase and anti-inflammatory activities by various in vitro assays. 37 and 30 metabolites belonging to different classes were identified by UHPLC-ESI-MS and GC�MS analyses respectively. Chemometric analysis revealed that P. lentiscus and P. khinjuk were more closely related chemically to each other. All studied Pistacia leaves extracts showed apparent anti-cholinesterase and anti-inflammatory activities, which promotes their use in the prevention and management of AD. � 2019 Elsevier B.V.
Comparative study of volatile oil content and antimicrobial activity of pecan cultivars growing in Egypt
(2013) El Hawary S.S.; Zaghloul S.S.; El Halawany A.M.; El Bishbishy M.H.; Department of Pharmacognosy; Faculty of Pharmacy; Cairo University; Cairo 11562; Egypt; Department of Pharmacognosy; Faculty of Pharmacy; Modern Sciences and Arts University; Giza; Egypt; Faculty of Pharmacy; King Abdulaziz University; Jeddah; Saudi Arabia
The volatile oils obtained from the leaves of four pecan cultivars growing in Egypt were evaluated for their chemical composition and antimicrobial activity. The selected cultivars (cv.) were Carya illinoinensis (Wangneh.) K. Koch. cv. Wichita, C. illinoinensis cv. Western Schley, C. illinoinensis cv. Cherokee, and C. illinoinensis cv. Sioux. The gas chromatography-mass spectrometry analyses revealed that the volatile oils from samples of the different cultivars differ in composition and percentage of their components. ?-Curcumene was found as the major constituent of the cv. Wichita oil, whereas germacrene D was the major component of cv. Sioux, cv. Cherokee, and cv. Western Schley. The antimicrobial activity was assayed using the Kirby-Bauer Method by measuring the zone of inhibition of growth. All volatile oils displayed an antimicrobial activity against the tested bacterial strains. On the other hand, only the volatile oil of cv. Wichita showed an antifungal effect on Aspergillus flavus. This work has identified candidates of volatile oils for future in vivo studies to develop antibiotic substitutes for the diminution of human and animal pathogenic bacteria. Nevertheless, the variations of the volatile oil components and antimicrobial potencies of the different studied cultivars, necessitate identifying the cultivars used in future studies. � 2013 Mary Ann Liebert, Inc., and Korean Society of Food Science and Nutrition.
LC-MS/MS-based chemometric analysis of phytochemical diversity in 13 Ficus spp. (Moraceae): Correlation to their in vitro antimicrobial and in silico quorum sensing inhibitory activities
(Elsevier B.V., 2018) Elhawary S.S.; Younis I.Y.; El Bishbishy M.H.; Khattab A.R.; Department of Pharmacognosy; Faculty of Pharmacy; Cairo University; Cairo; 11562; Egypt; Department of Pharmacognosy; Faculty of Pharmacy; MSA University; Giza; 12585; Egypt; Department of Pharmacognosy; Division of Pharmaceutical Sciences; College of Pharmacy; Arab Academy for Science; Technology and Maritime Transport; Alexandria; 1029; Egypt
Thirteen Ficus species, family Moraceae, were profiled via HPLC-ESI-MS/MS. About 35 metabolites were tentatively identified, for the first time, belonging to various classes including flavonoids (flavonols, flavanols, flavanonols, flavones, flavanones), flavonolignans, anthocyanins and hydroxycinnamic acids derivatives). We herein also report three species, viz. Ficus auriculata, Ficus lutea Vahl. and Ficus trigonata L., with promising antimicrobial activity for the management of the infectious diseases associated with the tested food-borne and spoilage microorganisms using agar well diffusion assay. The compositional variabilities existing among the studied species in correlation to their antimicrobial activities were explored using different chemometric tools. From the Orthogonal Projection to Latent Structures (OPLS) regression model, a correlation between the observed antimicrobial activity and the abundant Ficus phytoconstituents viz. quercetin-3-glucoside, kaempferol-3-O-rutinoside, kaempferol-3-O-glucoside, rutin and chlorogenic acid, was evidenced. By showing promising in silico anti-quorum sensing efficacy, these phytoconstituents could represent a promising set of natural hence safe antimicrobial agents. 2018
Looking at marine-derived bioactive molecules as upcoming anti-diabetic agents: A special emphasis on PTP1B inhibitors
(MDPI AG, 2018) Ezzat, Shahira M; El Bishbishy M.H.; Habtemariam S.; Salehi B.; Sharifi-Rad M.; Martins N.; Sharifi-Rad J.; Pharmacognosy Department; Faculty of Pharmacy; Cairo University; Kasr El-Ainy Street; Cairo; 11562; Egypt; Department of Pharmacognosy; Faculty of Pharmacy; October University for Modern Science and Arts (MSA); Cairo; 12566; Egypt; Herbal Analysis Services UK; Pharmacognosy Research Laboratories; University of Greenwich; Central Avenue; Chatham-Maritime; Kent; ME4 4TB; United Kingdom; Student Research Committee; Bam University of Medical Sciences; Bam; 44340847; Iran; Department of Medical Parasitology; Zabol University of Medical Sciences; Zabol; 61663-335; Iran; Institute for Research and Innovation in Health (i3S); University of Porto; Porto; 4200-135; Portugal; Faculty of Medicine; University of Porto; Alameda Prof. Hern�ni Monteiro; Porto; 4200-319; Portugal; Zabol Medicinal Plants Research Center; Zabol University of Medical Sciences; Zabol; 61615-585; Iran; Department of Chemistry; Richardson College for the Environmental Science Complex; University of Winnipeg; 599 Portage Avenue; Winnipeg; MB R3B 2G3; Canada
Diabetes mellitus (DM) is a chronic metabolic disease with high morbimortality rates. DM has two types: type 1, which is often associated with a total destruction of pancreatic beta cells, and non-insulin-dependent or type 2 diabetes mellitus (T2DM), more closely associated with obesity and old age. The main causes of T2DM are insulin resistance and/or inadequate insulin secretion. Protein-tyrosine phosphatase 1B (PTP1B) negatively regulates insulin signaling pathways and plays an important role in T2DM, as its overexpression may induce insulin resistance. Thus, since PTP1B may be a therapeutic target for both T2DM and obesity, the search for novel and promising natural inhibitors has gained much attention. Hence, several marine organisms, including macro and microalgae, sponges, marine invertebrates, sea urchins, seaweeds, soft corals, lichens, and sea grasses, have been recently evaluated as potential drug sources. This review provides an overview of the role of PTP1B in T2DM insulin signaling and treatment, and highlights the recent findings of several compounds and extracts derived from marine organisms and their relevance as upcoming PTP1B inhibitors. In this systematic literature review, more than 60 marine-derived metabolites exhibiting PTP1B inhibitory activity are listed. Their chemical classes, structural features, relative PTP1B inhibitory potency (assessed by IC50 values), and structure�activity relationships (SARs) that could be drawn from the available data are discussed. The upcoming challenge in the field of marine research�metabolomics�is also addressed. � 2018 by the authors.
Profile of bioactive compounds of Capparis spinosa var.Aegyptiaca growing in Egypt
(Sociedade Brasileira de Farmacognosia, 2016) Bakr R.O.; El Bishbishy M.H.; Pharmacognosy Department; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA); Giza; Egypt
The present study was designed to investigate polyphenolic and sulphur contents of the aerial parts of Capparis spinosa var.aegyptia (Lam.) Boiss., Capparaceae, wildly growing in Egypt.The chemical compositions of the water distilled essential oil were investigated by GC/MS analysis where the major constituent of the oil was methyl isothiocyanate (24.66%).Hydroethanolic extract was evaluated by LC-HRESI-MS-MS in both positive and negative modes.Forty-two compounds were identified including quercetin, kaempferol and isorhamnetin derivatives in addition to myricetin, eriodictyol, cirsimaritin and gallocatechin derivatives.Quercetin tetrahexoside dirhamnoside as well as kaempferol dihexoside dirhamnoside have not been identified before in genus Capparis.Phenolic acids, such as quinic acid, p-coumaroyl quinic acid and chlorogenic acid were also identified.Evaluation of cytotoxic activity of hydroethanolic extract against three human cancer cell lines (MCF-7; breast adenocarcinoma cells, Hep-G2; hepatocellular carcinoma cells and HCT-116; colon carcinoma) using 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay showed significant effect with IC50 values 24.5, 24.4 and 11 g/ml, compared to Doxorubicin as a standard cytotoxic drug.C.spinosa revealed itself as a promising candidate for nutraceutical researches. 2016 Sociedade Brasileira de Farmacognosia.
A putative Chondroprotective role for IL-1β and MPO in herbal treatment of experimental osteoarthritis
(BioMed Central Ltd., 2017) Aborehab N.M.; El Bishbishy M.H.; Refaiy A.; Waly N.E.; MSA University; Department of Biochemistry; Faculty of Pharmacy; Giza; 11787; Egypt; MSA University; Department of Pharmacognosy; Faculty of Pharmacy; Giza; 11787; Egypt; Assiut University; Department of Pathology Faculty of Medicine; Assiut; 71515; Egypt; Department of Physiology; Faculty of Medicine; Helwan University; Cairo; 11795; Egypt
Background: Herbal treatment may have a chondroprotective and therapeutic effect on Osteoarthritis (OA). We investigated the mechanism of action of ginger and curcumin rhizomes cultivated in Egypt in treatment of OA in rat model. Methods: Thirty-five albino rats were intra-articularly injected with Monosodium Iodoacetate in the knee joint. Ginger and curcumin was orally administered at doses of 200 and 400mg/kg (F200 and F400). Serum levels of cartilage oligomeric matrix protein (COMP), hyaluronic acid (HA), malondialdehyde (MDA), myeloperoxidase (MPO), Interleukin-1 beta (IL-1?) and superoxide dismutase activity (SOD) were measured using ELISA. The composition of the herbal formula hydro-ethanolic extract was characterized using UPLC-ESI-MS. Histopathological changes in injected joints was examined using routine histopathology. Statistical analysis was performed using one-way ANOVA. Results: Serum levels of COMP, HA, MPO, MDA, and IL-1? were significantly decreased in F 200, F 400 and V groups when compared to OA group (P value <0.0001). On the other hand SOD levels were significantly elevated in treated groups compared to OA groups (P value <0.0001). Conclusions: The ginger/curcumin at 1:1 had chondroprotective effect via anti-inflammatory and antioxidant effect in rat OA model. Further pharmacological and clinical studies are needed to evaluate this effect. � 2017 The Author(s).
Resistin mediates tomato and broccoli extract effects on glucose homeostasis in high fat diet-induced obesity in rats
(BioMed Central Ltd., 2016) Aborehab N.M.; El Bishbishy M.H.; Waly N.E.; Faculty of Pharmacy; MSA University; Department of Biochemistry; Giza; 11787; Egypt; Faculty of Pharmacy; MSA University; Department of Pharmacognosy; Giza; 11787; Egypt; Faculty of Medicine; Helwan University; Department of Physiology; Helwan; 11787; Egypt
Background: Resistin is an adipocyte hormone that regulates glucose metabolism. Elevated levels of resistin may cause insulin resistance. This may link obesity, and increased fat mass to type II diabetes and insulin resistance. We hypothesized that treatment with tomato and broccoli extracts regulates glucose homeostasis via modulation of resistin levels in high fat diet-induced obesity rats (HFD). Methods: Forty-eight male albino rats were divided into 8 groups as follows: control, HFD, stop fat diet (SD), Tomato 200 mg/kg (T200), Tomato 400 mg/kg (T400), Broccoli 200 mg/kg (B200), Broccoli 400 mg/kg (B400), and Chromax (CX). Treatment continued for 1 month. Serum levels of resistin, leptin, adiponectin, glucose and insulin were measured using ELISA and spectrophotometry. Results: Serum levels of resistin were significantly reduced in the T 200, T 400, B 200, B 400 and CX groups to: 4.13 � 0.22 ng/ml, 1.51 � 0.04 ng/ml, 4.13 � 0.22 ng/ml, 2.32 � 0.15 ng/ml and 1.37 � 0.03 ng/ml, respectively, compared to HFD group and SD group (P value < 0.0001). Non-significant differences were found between T 400, B 400 and CX groups. Serum levels of leptin were significantly reduced in the T 400 (22.7 � 0.84 pg/ml) group compared to the B 400 (41 � 2.45 Pg/ml) and CX groups (45.7 � 2.91 Pg/ml), P value < 0.001. Serum levels of adiponectin were significantly increased in the T 400 group (131 � 3.84 pg/ml) compared to the CX group (112 � 4.77 pg/ml), P value < 0.01. Conclusions: Our results demonstrate that tomato and broccoli extract treatment regulates glucose homeostasis via reduction of serum resistin and may be a useful non-pharmacological therapy for obesity. � 2016 The Author(s).