Browsing by Author "Diab A."

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Antimicrobial activity of hybrids terpolymers based on magnetite hydrogel nanocomposites
(MDPI AG, 2019) Farag R.K.; Labena A.; Fakhry S.H.; Safwat G.; Diab A.; Atta A.M.; Petroleum Application Department; Egyptian Petroleum Research Institute (EPRI); Nasr City; Cairo; 11727; Egypt; Faculty of Biotechnology; October University for Modern Science and Arts; 26 July Mehwar Road intersection with Wahat Road; 6th October City P.O. Box 2511; Egypt; Chemistry Department; College of Science; King Saud University; P.O. Box 2455; Riyadh; 11451; Saudi Arabia
In the past few years, the development of hydrogel properties has led to the emergence of nanocomposite hydrogels that have unique properties that allow them to be used in various different fields and applications such as drug delivery, adsorption soil containing, tissue engineering, wound dressing, and especially antimicrobial applications. Thus, this study was conducted in order to fabricate a novel crosslinked terpolymer nanocomposite hydrogel using the free radical copolymerization method based on the usage of 2-acrylamido-2-methylpropane sulfonic acid (AMPS), acrylamide (AAm), acrylonitrile (AN), and acrylic acid (AA) monomers and iron oxide (Fe3O4) magnetic nanoparticles and using benzoyl peroxide as an initiator and ethylene glycol dimethacrylate (EGDMA) as a crosslinker. The structure of the synthesized composite was confirmed using Fourier transform infrared (FTIR) spectroscopy and x-ray powder diffraction (XRD) measurements. Furthermore, the surface morphology and the magnetic nanoparticle distributions were determined by scanning electron microscopy (SEM) measurement. In addition, the swelling capacity of the hydrogel nanocomposite was measured using the swelling test. Lastly, the efficiency of the produced composite was evaluated as an antimicrobial agent for Gram-positive and Gram-negative bacterial strains and a fungal strain. � 2019 by the authors.
Evaluation of Mir-224, Mir-215 and Mir-143 as Serum biomarkers for HCV associated Hepatocellular carcinoma
(Asian Pacific Organization for Cancer Prevention, 2017) Mamdouh S.; Khorshed F.; Aboushousha T.; Hamdy H.; Diab A.; Seleem M.; Saber M.; Department of Biochemistry and Molecular Biology; Theodor Bilharz Research Institute; October University for Modern Sciences and Arts; Giza; Egypt; Department of Pathology; October University for Modern Sciences and Arts; Giza; Egypt; Department of Surgery; Theodor Bilharz Research Institute; October University for Modern Sciences and Arts; Giza; Egypt; Faculty of Biotechnology; October University for Modern Sciences and Arts; Giza; Egypt; Department of Surgery; National Hepatology and Tropical Medicine Research Institute; Cairo; Egypt
HCV induced hepatitis and hepatocellular carcinoma as its sequel are major health problems world-wide and especially in Egypt. For diagnosis and during treatment of liver diseases, liver functions are monitored through determination of serum levels of liver enzymes and a-fetoprotein although the obtained information is generally not sufficient for either early detection of hepatic insult or effective follow up of therapeutic effects. More sensitive biomarkers may help to achieve these goals. MiRNAs are small non-coding RNAs that have an important role in gene expression and regulation. Many, such as miR-224, miR-215, miR-143 are correlated with tumor appearance and with the degree of fibrosis in lung, breast and colon cancer. This study was performed to estimate the level of these miRNAs in serum of patients with HCV-associated hepatitis and HCC in relation to grade of hepatitis, stage of fibrosis and differentiation of tumor tissue. In addition, correlations between serological and tissue levels were assessed. A total of 80 patients were examined, out of which 50 were included in the study. Blood samples and tissue specimens from malignant tumor and corresponding non-tumor tissue of HCV hepatitis patients were collected. Blood samples from 20 healthy volunteers were also obtained as controls. It was found that miRNAs profiles differed in HCC patients compared to controls and HCV-associated hepatitis cases. Distinction of tumor grade and fibrosis stage of patients as well as between different grades of tumor differentiation proved possible, making miRNAs promising biomarkers for diagnosis and assessment of treatment response of HCC patients.
The prognostic significance of the long non-coding RNAs “CCAT1, PVT1” in t(8;21) associated Acute Myeloid Leukemia
(Elsevier B.V., 2019) El-Khazragy N.; Elayat W.; Matbouly S.; Seliman S.; Sami A.; Safwat G.; Diab A.; Clinical Pathology and Hematology Department; Faculty of Medicine; Ain Shams University Biomedical Research Department; P.O. Box 11381; Cairo; Egypt; Department of Medical Biochemistry; Faculty of Medicine; Ain Shams University; Egypt; Department of Pediatrics; Faculty of Medicine; Ain Shams University; Egypt; Faculty of Biotechnology; October University for Modern Sciences and Arts (MSA); Cairo; Egypt
Long non-coding RNA (LncRNA) is recently linked to various types of cancers, CCAT and PVT1 are two LncRNAs linked to t(8;21) associated Acute Myeloid Leukemia, the interplay between CCAT, PVT1 and the MYC proto-oncogene implicated in t(8;21) could present an opportunity for using LncRNA as prognostic biomarker or a target for therapy, We investigated the expression levels of LncRNAs in 70 patients; 30 with t(8;21) positive AML and 40 with t(8;21) negative AML, We found that CCAT1 and PVT1 are expressed in higher levels in t(8;21) positive �AML by 5.3 folds compared to t(8;21) negative group; the expression values were significantly associated with high-risk clinical criteria; moreover, they are associated with lower overall survival (OS) rate and leukemia-free survival (LFS), however we didn't find a statistically significant cut-off value of LncRNAs using the Cox regression analysis for Lnc_PVT1 except with LFS, we conclude that high expression levels of CCAT1 and PVT1 are associated with poor prognosis while being poor prognostic biomarkers in t(8;21) associated AML. � 2019 Elsevier B.V.