Browsing by Author "Bayoumi F.S."

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Circulating microRNAs as potential non-invasive biomarkers in pediatric patients with celiac disease
(Elsevier B.V., 2019) Amr K.S.; Bayoumi F.S.; Eissa E.; Abu-Zekry M.; Medical molecular genetics Department; National Research Centre; Cairo; Egypt; Immunogenetics Department; National Research Centre; Cairo; Egypt; Microbiology and Immunology Department; MSA University; Egypt; Immunogenetics Department; National Research Centre; Cairo; Egypt; Abu El Reesh Children�s Hospital; Cairo University; Cairo; Egypt
Celiac disease is an enteropathy induced by ingestion of gluten triggering an immune response in genetically predisposed individuals. MiRNAs are small non-coding RNAs that have a role as regulators of gene expression at the post transcriptional level. The aim of this study is to evaluate the possibility of using circulating miRNAs as non-invasive biomarkers in pediatric patients with celiac disease. In addition, we examine the effect of a gluten-free diet on the expression of these miRNAs in serum of CD patients. The expression pattern of miR-21 and miR-31 was estimated in serum of 25 untreated CD patients (recently diagnosed), 25 treated CD patients (on gluten-free diet) and 20 healthy controls using qRT-PCR. Our results demonstrated the significant up-regulation of microRNA-21 in the untreated celiac patients in comparison with the treated group and healthy controls. Moreover, miR-31 expression was significantly under-expressed in the untreated celiac patients in comparison with the treated group and healthy controls. Furthermore, the results showed that miR-21 expression level was significantly positively correlated with the tTG IgA auto-antibodies. In conclusion, circulating miRNA-21 and miRNA-31 could serve as potential non-invasive biomarkers for pediatric CD patients. � 2019, EDRA S.p.A. All rights reserved.
Clinical and laboratory profile of urinary tract infections associated with extended spectrum-lactamase producing Escherichia coli and Klebsiella pneumoniae
(International Journal of Green Economics, 2016) Al Yousef S.A.; Younis S.; Farrag E.; Moussa H.S.; Bayoumi F.S.; Ali A.M.; Department of Medical Laboratory Technology; College of Applied Medical Sciences; University of Hafr Al Batin; Hafr Al Batin; Saudi Arabia; Faculty of Women for Art; Science and Education; Ain Shams University; Egypt; Microbiology Department; Faculty of Pharmacy; MSA University; Cairo; Egypt
Background. Urinary tract infection (UTI) is mainly due to invasion of the urethra, bladder or kidneys by pathogens. The emergence of extended spectrum �-lactamases (ESBL) is responsible for frequently observed empirical therapy failures. Objectives. To study the clinical and laboratory characteristics of UTIs caused by ESBL producing Escherichia coli (E. coli) and Klebsiella pneumonia (K. pneumonia). Methods. A cross-sectional clinical and laboratory study was performed at King Khalid Hospital, Hafr Al Batin, Saudi Arabia between March 2014 to October 2015. A total of 908 urine samples from suspected UTI patients was collected. Samples were isolated on Cysteine Electrolyte-Deficient (CLED) agar. Positive cultures were identified and tested for antimicrobial susceptibility by MicroScan WalkAway-96 SI System, and then ESBL was confirmed by double disc synergy test (DDST) and phenotypic confirmatory disc diffusion test (PCDDT). Results. A total of 680 samples (288 males and 392 females) were culture positive. 520 samples (76.5%)of E. Coli were found and 160 samples of K. pneumonia were identified (23.5%). ESBL testing showed 296 (218 E. coli and 78 K. pneumonia) samples of positive isolates. Non-ESBL isolates showed highest resistance to ampicillin followed by Mezocillin and Trimethoprim-Sulphamethoxazole- which are usually recommended as the initial treatment of UTI-while ESBL isolates showed resistance to third generation cephalosporin along with Ampicillin and Trimethoprim-Sulphamethoxazole. In this study, four significant risk factors for ESBL infection such as diabetes, recurrent UTI, previous use of antibiotics and previous hospitalization were found. Conclusion. Identifying the risk factors and antibiotic susceptibility patterns associated with ESBL producing E. coli and K. pneumonia is a useful guide for treatment strategy and control of ESBL UTI. 2016 by the Association of Clinical Scientists, Inc.
The effect of 3D-printed polycaprolactone (PCL) scaffold with different surface coatings on the immunogenicity of stem cells from human exfoliated deciduous teeth (SHED)
(Nova Science Publishers, Inc., 2018) Ahmed N.E.-M.B.; El Azeem A.F.A.; Bayoumi F.S.; Department of Oro-Dental Genetics; Medical Research Centre of Excellence; National Research Centre; Cairo; Egypt; Stem Cell Laboratory; Center of Excellence for Advanced Sciences; National Research Centre; Cairo; Egypt; Department of Microbiology and Immunology; Faculty of Pharmacy; October University of Modern Sciences and Arts (MSA); Cairo; Egypt; Department of Immunogenetics; National Research Centre; Cairo; Egypt
Biomaterials have allowed many advances in the field of bone tissue engineering (BTE). Polycaprolactone (PCL) is an FDA degradable polymer that has been used for manufacturing scaffolds in bone tissue engineering. Different modifications have been made to PCL scaffold in order to improve its surface properties and osteoinductive abilities. It is essential that any modification of the engineered scaffold should avoid altering the properties of the seeded cells. Stem cells isolated from human exfoliated deciduous teeth (SHED)-similar to all other mesenchymal stem cells (MSCs)-do not express costimulatory molecules on their surface. These molecules are essential for the completion of T cell activation and therefore the lack of their expression accounts for the low immunogenicity of MSCs. In this study, SHED were isolated and seeded on 3D-printed PCL scaffolds which were either non-coated or coated with either nanohydroxyapatite (N-HAp) or multiwalled carbon nanotubes (MWCNTs). Cells cultured without scaffolds were used as a control. After three-day culture, all cells were collected and analyzed by flow cytometry for the expression of surface co-stimulatory molecules; CD40, CD80, and CD86. Results showed that different coating materials evidently affected the immune-genicity of the seeded SHED. The expression of CD40, CD80, and CD86 markers was significantly higher in cells seeded on MWCNTs/PCL scaffolds, followed by cells seeded on N-HAp/PCL scaffolds when compared to cells seeded on non-coated PCL scaffolds. On the other hand, cells seeded on non-coated PCL scaffolds showed no significant difference in their expression to the specified markers when compared to the control group. The data presented in this study are significant when considering allogeneic MSCs treatments in order to avoid immune rejection. � Nova Science Publishers, Inc.
Evaluation of genexpert MTB/RIF assay for direct diagnosis of pulmonary tuberculosis
(Saudi Arabian Armed Forces Hospital, 2016) Moussa H.S.; Bayoumi F.S.; Ali A.M.; Botany Department; Faculty of Women for Art; Science; and Education; Ain Shams University; Cairo; Egypt; Microbiology Department; Faculty of Pharmacy; Modern Sciences and Arts University; Cairo; Egypt; Department of Medical Laboratory Technology; College of Applied Medical Science; University of Dammam; Hafr Al Batin; Saudi Arabia
Objectives: To evaluate the performance of GeneXpert MTB/RIF assay for direct diagnosis of pulmonary tuberculosis (PTB). Methods: This is a cross-sectional study conducted between October 2013 and February 2016 at Abbassaia Chest Hospital and Ain Shams University Hospital, Cairo, Egypt. Inclusion criteria were adults between 18 and 60 years with suspected PTB and classified into 5 clinical categories based on their clinical, radiological, and laboratory findings: confirmed TB, probable TB, possible TB, unlikely TB, and not TB. Two sputum samples from each participant were analyzed by GX and the results were compared by conventional culture. Results: In total, 218 participants were enrolled: 71 had confirmed TB; 112, highly probable TB; 20, probable TB; 10, unlikely TB; and 5, no TB. The sensitivity and specificity of the GX assay were 93% and 98.3% respectively. GeneXpert was positive in 93% of confirmed TB and 2.2% of probable TB cases. Conclusions: GeneXpert is a rapid and promising technique with good sensitivity (93%) and specificity (98.3%), but it cannot be used as a standalone PTB diagnostic tool. There is a need for more GX evaluation studies in countries with low TB incidence. � 2016, Saudi Arabian Armed Forces Hospital. All rights reserved.
Gene Xpert for direct detection of Mycobacterium Tuberculosis in stool specimens from children with presumptive pulmonary tuberculosis
(Association of Clinical Scientists, 2016) Moussa H.S.; Bayoumi F.S.; Mohamed A.M.A.; Faculty of Women for Art; Science and Education; Ain Shams University; Cairo; Egypt; Microbiology Department; Faculty of Pharmacy; MSA University; Cairo; Egypt; Department of Immunogenetics; National Research Centre; Giza; Egypt; Egyptian Company of Serum and Vaccines; Giza; Egypt
Background. Gene Xpert(GX) is a novel real time polymerase chain reaction (RT-PCR) assay which was endorsed by the World Health Organization (WHO) in 2011 for tuberculosis (TB) diagnosis and susceptibility to refampicin(RIF). Objective. To evaluate GX for direct diagnosis of TB in stool samples from children with suspected pulmonary Tuberculosis (PTB). Methods. Children older than one year and younger than 16 years with presumptive PTB were enrolled and classified to five clinical categories based on clinical, radiological, and laboratory findings: Confirmed TB, probable TB, possible TB, Unlikely TB, and not TB. Two stool samples were collected from each child and tested for the presence of Mycobacterium tuberculosis (MTB) by GX and the obtained results were compared to Lowenstien-Jensen (LJ) culture as a gold standard. Results. In total, 115 children were enrolled. 36 had been confirmed with TB, 61 probably TB, 10 possible TB, 5 unlikely TB, and 3 not TB. GX had a sensitivity of 83.33 and 80.56 % and specificity of 98.73 and 99.36 % by patients and samples respectively. GX was positive in 83.3% of confirmed TB as well as 1.6 and 0.8% of probable TB cases by patients and samples respectively. Conclusions. GX provided timely results with quit acceptable sensitivity and good specificity compared to LJ culture. In this study, sensitivity calculations take into account only children with confirmed TB. GX could not detect TB in children with probable TB, so it should not be used alone for TB diagnosis. Further studies for GX stool protocol optimization and assessment is required. � 2016 by the Association of Clinical Scientists, Inc.
The role of microRNA-31 and microRNA-21 as regulatory biomarkers in the activation of T lymphocytes of Egyptian lupus patients
(Springer Verlag, 2016) Amr K.S.; Bayoumi F.S.; Elgengehy F.T.; Abdallah S.O.; Ahmed H.H.; Eissa E.; Head of Medical Molecular Genetics Department; National Research Centre; Cairo; Egypt; Immunogenetics Department; National Research Centre; Cairo; Egypt; Head of Microbiology and Immunology Department; MSA University; Cairo; Egypt; Department of Rheumatology and Rehabilitation; Faculty of Medicine; Cairo University; Cairo; Egypt; Faculty of Science; Cairo University; Giza; Egypt; Head of Medical Molecular Genetics Department; National Research Centre; El Buhouth St.; Cairo; Dokki 12622; Egypt
Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by familial aggregation and genetic predisposition. MicroRNAs (MiRNAs) serve as critical biomarkers in lupus patients because of their aberrant expression in different SLE stages. The study aimed to investigate the correlation of miR-31 and miR-21 with IL-2 in SLE patients as regulatory biomarkers in the activation of T lymphocytes of Egyptian lupus patients. Quantitative RT-PCR is carried out to estimate the expressions of miR-31 and miR-21, and IL-2 levels were determined using ELISA in plasma of 40 patients with SLE, 20 of their first-degree relatives and 20 healthy controls. The study also determined the systemic lupus erythematosus disease activity index (SLEDAI) score and proteinuria in SLE patients. The results revealed that miR-31 was lower expressed, while miR-21 was high expressed in SLE patients compared to their first-degree relatives and controls. MiR-31 was negatively correlated with SLEDAI and proteinuria in lupus patients, while miR-21 showed positive correlation with them. Also we found that there is a significant positive correlation between miR-31 and IL-2 in SLE patients, while miR-21 was negatively correlated with IL-2 level in patients. In conclusion, the study disclosed a significant association between miR-31 and miR-21 expression with IL-2 level in SLE patients. The regulatory biomarkers of miR-31 and miR-21 might have an impact on regulating IL-2 pathway expression and in turn on the activation of T lymphocytes in SLE. � 2016, Springer-Verlag Berlin Heidelberg.