Browsing by Author "Amr K.S."

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    Circulating microRNAs as potential non-invasive biomarkers in pediatric patients with celiac disease
    (Elsevier B.V., 2019) Amr K.S.; Bayoumi F.S.; Eissa E.; Abu-Zekry M.; Medical molecular genetics Department; National Research Centre; Cairo; Egypt; Immunogenetics Department; National Research Centre; Cairo; Egypt; Microbiology and Immunology Department; MSA University; Egypt; Immunogenetics Department; National Research Centre; Cairo; Egypt; Abu El Reesh Children�s Hospital; Cairo University; Cairo; Egypt
    Celiac disease is an enteropathy induced by ingestion of gluten triggering an immune response in genetically predisposed individuals. MiRNAs are small non-coding RNAs that have a role as regulators of gene expression at the post transcriptional level. The aim of this study is to evaluate the possibility of using circulating miRNAs as non-invasive biomarkers in pediatric patients with celiac disease. In addition, we examine the effect of a gluten-free diet on the expression of these miRNAs in serum of CD patients. The expression pattern of miR-21 and miR-31 was estimated in serum of 25 untreated CD patients (recently diagnosed), 25 treated CD patients (on gluten-free diet) and 20 healthy controls using qRT-PCR. Our results demonstrated the significant up-regulation of microRNA-21 in the untreated celiac patients in comparison with the treated group and healthy controls. Moreover, miR-31 expression was significantly under-expressed in the untreated celiac patients in comparison with the treated group and healthy controls. Furthermore, the results showed that miR-21 expression level was significantly positively correlated with the tTG IgA auto-antibodies. In conclusion, circulating miRNA-21 and miRNA-31 could serve as potential non-invasive biomarkers for pediatric CD patients. � 2019, EDRA S.p.A. All rights reserved.
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    The role of microRNA-31 and microRNA-21 as regulatory biomarkers in the activation of T lymphocytes of Egyptian lupus patients
    (Springer Verlag, 2016) Amr K.S.; Bayoumi F.S.; Elgengehy F.T.; Abdallah S.O.; Ahmed H.H.; Eissa E.; Head of Medical Molecular Genetics Department; National Research Centre; Cairo; Egypt; Immunogenetics Department; National Research Centre; Cairo; Egypt; Head of Microbiology and Immunology Department; MSA University; Cairo; Egypt; Department of Rheumatology and Rehabilitation; Faculty of Medicine; Cairo University; Cairo; Egypt; Faculty of Science; Cairo University; Giza; Egypt; Head of Medical Molecular Genetics Department; National Research Centre; El Buhouth St.; Cairo; Dokki 12622; Egypt
    Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by familial aggregation and genetic predisposition. MicroRNAs (MiRNAs) serve as critical biomarkers in lupus patients because of their aberrant expression in different SLE stages. The study aimed to investigate the correlation of miR-31 and miR-21 with IL-2 in SLE patients as regulatory biomarkers in the activation of T lymphocytes of Egyptian lupus patients. Quantitative RT-PCR is carried out to estimate the expressions of miR-31 and miR-21, and IL-2 levels were determined using ELISA in plasma of 40 patients with SLE, 20 of their first-degree relatives and 20 healthy controls. The study also determined the systemic lupus erythematosus disease activity index (SLEDAI) score and proteinuria in SLE patients. The results revealed that miR-31 was lower expressed, while miR-21 was high expressed in SLE patients compared to their first-degree relatives and controls. MiR-31 was negatively correlated with SLEDAI and proteinuria in lupus patients, while miR-21 showed positive correlation with them. Also we found that there is a significant positive correlation between miR-31 and IL-2 in SLE patients, while miR-21 was negatively correlated with IL-2 level in patients. In conclusion, the study disclosed a significant association between miR-31 and miR-21 expression with IL-2 level in SLE patients. The regulatory biomarkers of miR-31 and miR-21 might have an impact on regulating IL-2 pathway expression and in turn on the activation of T lymphocytes in SLE. � 2016, Springer-Verlag Berlin Heidelberg.