Browsing by Author "Al-Gendy A.A."

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Botanical and genetic characteristics of Lobularia libyca (viv). C.F.W. Meissn. (Brassicaceae)
(International Journal of Pharmacognosy and Phytochemical Research, 2014) Zaghloul S.S.; Nematallah K.A.; Al-Gendy A.A.; Ayoub N.; Pharmacognosy Department; October University for Modern Sciences and Arts (MSA); Giza; Egypt; Pharmacognosy Department; Zagazig University; Al-Sharkiya; Egypt; Pharmacognosy Department; Ain-Shams University; Cairo; Egypt; Pharmacognosy Department; BUE University; Cairo; Egypt
Lobularia libyca (viv). C.F.W. Meissn. is an annual herbaceous desert plant native to North Africa belonging to Family Brassicaceae (Cruciferae). Study of the botanical features was carried out for the root, the young and old stems, leaf, flower and seed of the plant. L. libyca was characterized by the presence of non-glandular branched unicellular two armed hair in the stem, leaf and flower while the root shows narrow cork and xylem occupies about 80% of the diameter of the root. The flower is characterized by hairy sepals and spherical pollen grains with three germ pores and three germ furrows. The pericarp is characterized by hairy epicarp and presence of fibrosclerides as innermost layer of the mesocarp. The septum shows pitted lignified parenchyma. The seed shows accumbent embryo and the testa is characterized by the presence of pigment layer with reddish brown content. The DNA of the plant was extracted from leaf samples and analyzed using eleven random decamer primers. A total of 89 random amplified polymorphic DNA (RAPD) markers were identified. Both the botanical study and the DNA fingerprint helped in the identification of the plant.
Glucosinolates profile, volatile constituents, antimicrobial, and cytotoxic activities of Lobularia libyca
(Institute of Electrical and Electronics Engineers Inc., 2016) Al-Gendy A.A.; Nematallah K.A.; Zaghloul S.S.; Ayoub N.A.; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA); Giza; Egypt; Faculty of Pharmacy; Zagazig University; Zagazig; Egypt; Faculty of Pharmacy; British University in Egypt; Cairo; Egypt; Faculty of Pharmacy; Ain-Shams University; Cairo; Egypt; Faculty of Medicine; Umm Al-Qura University; Makkah; Saudi Arabia
Context: Brassicaceae plants are associated with protection against cancers due to their glucosinolate contents. Objectives: We investigate fresh leaves, roots and ripe seeds of Lobularia libyca (Viv.) C.F.W. Meissn. (Brassicaceae) to identify their glucosinolate constituents, antimicrobial and cytotoxic activities Materials and methods: The glucosinolates were identified using GC-MS analysis of their hydrolysis products and LC-MS analysis in the case of seeds. Disc diffusion (1 mg/disc) and minimum inhibitory concentration (0�160 ?g/mL) methods were used to evaluate the antimicrobial activity of seed hydrolysate. In vitro cytotoxicity against colorectal HCT-116, hepatic HUH-7, breast MCF-7 and lung A-549 cells was evaluated for seed hydrolysate (0.01�100 ?g/mL) using the sulforhodamine B assay and doxorubicin as a standard Results: Three glucosinolates were identified for the first time in this plant and genus Lobularia. Glucoiberverin was the major compound accumulated in the seeds and leaves, while glucoiberin and glucoerucin were detected only in the seeds. No glucosinolates were detected in roots under the same experimental conditions. Other volatile constituents, e.g., terpenes and fatty acids were only identified in the seeds. The seed hydrolysate showed significant antimicrobial activities against Candida albicans and Pseudomonas aeruoginosa (MIC = 64 and 82 ?g/mL, respectively). The seed hydrolysate exhibited a marked selective cytotoxicity in vitro against colorectal, hepatic and breast cancer cell lines. The IC 50 values were 0.31, 2.25 and 37 ?g/mL, respectively. Discussion and conclusion: The results indicated the antimicrobial activity of L. libyca and the selective effect of the seed hydrolysate as a cytotoxic drug that is potentially more active than doxorubicin against HCT-116. � 2016 Informa UK Limited, trading as Taylor & Francis Group.
Phenolic profile, anti-inflammatory, antinociceptive, anti-ulcerogenic and hepatoprotective activities of Pimenta racemosa leaves
(BioMed Central Ltd., 2018) Moharram F.A.-E.; Al-Gendy A.A.; El-Shenawy S.M.; Ibrahim B.M.; Zarka M.A.; Helwan University; Department of Pharmacognosy; Faculty of Pharmacy; Cairo; 11795; Egypt; Zagazig University; Department of Pharmacognosy; Faculty of Pharmacy; Zagazig; 44519; Egypt; National Research Center; Department of Pharmacology; Dokki; Cairo; 12622; Egypt; Modern Sciences and Arts University; Department of Pharmacognosy; Faculty of Pharmacy; 6 October City; Egypt; Modern University for Technology and Information; Department of Pharmacognosy; Faculty of Pharmacy; Cairo; Egypt
Background: Pimenta racemosa tree has many traditional uses where its leaves are used as herbal tea for treatment of flatulence, gastric disorder, osteoarthritis, colds and fever in addition to its analgesic and anti-inflammatory activities. So, this study aimed to isolate phenolic constituents of 80% aqueous methanol extract (AME) of leaves and evaluate its biological activities. Methods: The defatted AME was chromatographed and structures of the isolated compounds were elucidated using UV, NMR spectroscopy and UPLC-ESI-MS analysis. Antioxidant activity was investigated using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity. Anti-inflammatory activity was evaluated using carrageenan - induced paw oedema, while antinociceptive activity was determined by chemical and thermal stimuli. Anti-ulcerogenic effect of AME against gastric damage induced by ethanol in Wister male albino rats was evaluated. Also, hepatoprotective activity was investigated through determination of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) following oral administration of paracetamol. Both of Anti-ulcerogenic and hepatoprotective activities (125, 250 and 500 mg/kg b.wt.) were supported by histopathological examinations. Results: Gallic acid (1), methyl gallate (2), avicularin (3), quercetin 3-O-?-D-arbinopyranoside (4), quercetin 3-O-?-D-glucopyranoside (5), quercetrin (6), cynaroside (7), strictinin (8), castalagin (9), grandinin (10) quercetin (11) and ellagic acid (12) were isolated. AME showed significant radical scavenging activity (SC50 = 4.6 ?g/mL), promising anti-inflammatory effect through inhibition of oedema and antinociceptive activity by reduction in number of writhes after acetic acid injection and prolongation of reaction time towards the thermal stimulus. AME reduced the gastric mucosal lesions compared with ethanol control and ranitidine groups, ALT at the three doses and AST only at 125 and 250 mg/kg b.wt., when compared with paracetamol group. The results were confirmed by histopathological studies. Conclusion:P. racemosa leaves are rich in phenolic compounds and showed significant biological activities. � 2018 The Author(s).