Browsing by Author "A. Salem, Mohamed"

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    BIOLOGICAL ACTIVITY AND STANDARDIZATION OF THE ETHANOLIC EXTRACT OF THE AERIAL PARTS OF MENTHA SUAVEOLENS EHRH
    (World Journal of Pharmacy and Pharmaceutical Sciences, 2014) Ezzat, Shahira M; I. El-Askary, Hesham; A. El-Kashoury, El-Sayeda; A. Kandil, Zeinab; A. Salem, Mohamed
    A biologically guided fractionation of the ethanolic extract of the aerial parts of Mentha suaveolens Ehrh. cultivated in Egypt revealed that the ethyl acetate fraction had the highest hepatoprotective activity as it prevented the increase caused by CCl4 in the levels of aspartate aminotransferase (AST), alanine amino transferase (ALT) and alkaline phosphatase (ALP) enzymes by 51.6%, 57.0% and 56.7%, respectively. The same fraction also showed the highest antioxidant activity; in vivo (as it restored the glutathione level in diabetic rats by 98.0 %) and in vitro as it had the highest free radical scavenging activity (IC50=31g/ mL). It also had the highest cytotoxic activity against human liver carcinoma cell line (HEPG2) (IC50 = 5.1 μg/ mL). Consequently, this fraction was purified to yield caffeic acid and rosamarinic acid. Rosmarinic acid, the major component possessed cytotoxic activity on HEPG2 (IC50 = 4.5 μg/ mL) comparable to doxorubicin (IC50 = 3.73 μg/mL). A rapid reversed-phase high-performance liquid chromatography (RP-HPLC) method was developed for the standardization of the active ethyl acetate fraction and the method was validated. Rosmarinic acid content in M. suaveolens was estimated by RPHPLC, to be 1.16 and 50.17% w/w for the powdered aerial parts and ethyl acetate fraction, respectively.
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    Chemical and biological study of Mentha suaveolens Ehrh. cultivated in Egypt
    (Journal of Medicinal Plant Research, 2014) Ezzat, Shahira M; A. El-Kashoury, El-Sayeda; A. Kandil, Zeinab; A. Salem, Mohamed; A. Sleem, Amany
    Investigation of the different fractions of the ethanolic extract of the aerial parts of M. suaveolens growing in Egypt yielded nine compounds; two new triterpenes [3β-acetyl -22α-hydroxy ursa-12,20- diene (compound 1) and 2α, 3β-dihydroxy-olean-18-en-29-oic acid (compound 7)] and nine known compounds: a sterol and its glucoside; β-sitosterol (compound 2) and β-sitosterol-3-O- β-D-glucoside (compound 4), a triterpene; oleanolic acid (compound 3), two monoterepenes; dihydrolimonene (compound 5) and 7-hydroxy-p-cymene (compound 6), two flavonoids; isoquercitrin (compound 8) and rutin (compound 9) which were isolated from M. suaveolens Ehrh. for the first time. The structures of the isolated compounds were identified by spectral data (UV, MS, 1D and 2D-NMR) and comparison with authentic samples. Moreover, the ethanolic extract showed potent analgesic activity as compared to indomethacin. The ethyl acetate fraction was the most potent as anti-inflammatory (88%), followed by the ethanolic extract (82.9%) as compared with indomethacin. The ethanolic extract and its four subfractions showed a moderate inhibitory activity against the tested human pathogenic bacteria.