Browsing by Author "A. Choucry, Mouchira"

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    Antibacterial, antioxidant, and topical antiinflammatory activities of Bergia ammannioides: A wound-healing plant
    (Informa Healthcare, 2015) Ezzat, Shahira M; A. Choucry, Mouchira; A. Kandil, Zeinab
    Context: Despite the traditional use of Bergia ammannioides Henye ex Roth. (Elatinaceae) for the treatment of wounds in India, there is a scarcity of scientific data supporting this use. Objective: The objective of this study is to assess wound-healing potentiality of the plant, to study pharmacological activities that may contribute in eliminating wound complications, and to investigate the biologically active fractions. Material and methods: The ethanolic extract (EtOH) of the aerial parts was fractionated to obtain n-hexane (HxFr), chloroform (ClFr), ethyl acetate (EtFr), and n-butanol (BuOH) fractions. EtOH and its fractions were formulated in strength of 5 and 10% w/w ointment and tested for wound-healing activity using the excision model. The topical anti-inflammatory, in vitro antioxidant, and antibacterial activities were evaluated. HxFr and EtFr were chemically investigated to isolate their constituents. Results: Application of EtOH, HxFr, and EtFr (10% w/w ointments) leads to 71.77, 85.62, and 81.29% healing of the wounds with an increase in the collagen content. HxFr had the strongest anti-inflammatory (64.5% potency relative to Voltaren) and antibacterial activity (MIC ¼ 104 mg/ml against Staphylococcus aureus), while EtFr showed the strongest antioxidant activity against DPPH, ABTS+ , and super oxide radical with an IC50 value of 10.25 ± 0.01, 66.09 ± 0.76, and 167.33 ± 0.91 mg/ml, respectively. b-Sitosterol, lupeol, cyclolaudenol, and cycloartenol were isolated from HxFr. Quercetin, ellagic acid, kaempferol-3-O-a-L-rhamnoside, and quercetin-3-O-a-L-rhamnoside were isolated from EtFr. Discussion and conclusion: Our study presents scientific evidence for the efficacy of B. ammannioides in enhancing wound healing, and the first isolation of cyclolaudenol and cycloartenol from Bergia