Faculty Of Dentistry Research Paper

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Browsing Faculty Of Dentistry Research Paper by Author "6th October City"

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    Bond durability of different resin cements to caries-affected dentin under simulated intrapulpal pressure
    (IEEE, 2015) Mohamed M.F.; El Deeb H.A.; Gomaa I.E.; Mobarak E.H.; Operative Dentistry; Modern Science and Arts University; 6th October City; Egypt; Restorative Dentistry Department; Faculty of Oral and Dental Medicine; Cairo University; Cairo; Egypt; Department of Pharmaceutical Biology and Biotechnology; German University in Cairo-GUC; Cairo; Egypt
    Objective: To evaluate the durability of the bond of different resin cement systems to normal dentin (ND) and caries-affected dentin (CAD) with and without simulated intrapulpal pressure (IPP). Methods and Materials: Molars with midcoronal caries were used. Occlusal enamel was cut to expose both dentin substrates (ND and CAD). Dentin substrates were differentiated using visual, tactile, caries-detecting dye, and dye-permeability methods. Prepared crown segments were equally divided according to the tested resin cement systems: etch-andrinse resin cement, self-etch resin cement containing methacryloyloxydecyl dihydrogen phosphate (MDP), and self-adhesive resin cement. In addition to the dentin substrates and the resin cement types, the effect of application/storage conditions (with or without simulated IPP and with or without thermocycling) were tested. A microtensile bond strength test was done using a universal testing machine. Failure modes were determined using a scanning electron microscope. Results: Etch-and-rinse resin cement strength values were significantly affected by the difference in the dentin substrates as well as the different application/storage conditions. Selfetch adhesive containing MDP bonded equally to ND and CAD and remained stable under all tested conditions. Self-adhesive resin cement revealed a similar bond to ND and CAD; however, its values were the lowest, especially when IPP and thermocycling were combined. Mixed failure was the predominant failure mode. Conclusions: Etch-and-rinse resin cement was sensitive to dentin substrate and application/storage conditions. Resin cement with selfetch adhesive containing MDP revealed more reliable bonding to ND/CAD even when IPP and thermocycling were combined. The bonding of the self-adhesive resin cement could not compete with other resin cements. � Operative Dentistry.
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    The diagnostic and prognostic value of salivary sCD44 level determination in oral malignant and potentially premalignant lesions
    (2012) Ghalwash D.M.; Gaaly K.E.; Zahran F.M.; Shaker O.; El-Fol H.A.; Department of Oral Medicine; Periodontology and Radiology; Faculty of Dentistry; MSA University; 6th October City; Egypt; Department of Oral Medicine and Periodontology; Faculty of Oral and Dental Medicine; Cairo University; Egypt; Department of Oral Medicine and Periodontology; Faculty of Oral and Dental Medicine; Cairo University; Gedda; Saudi Arabia; Department of Oral Medicine; Faculty of Dentistry; King Abdul Aziz University; Gedda; Saudi Arabia; Department of Biochemistry; Faculty of Medicine; Cairo University; Egypt; Department of Surgical Oncology; Hospitals of Menoufia University; Egypt
    A key factor in the lack of improvement in prognosis of oral squamous cell carcinoma (OSCC) lesions over the years is the fact that a significant proportion are not diagnosed or treated until they reach an advanced stage. A molecular marker for malignant transformation in innocent looking oral lesions and a monitor for the aggressiveness of malignant lesions might be of help. The present study included 40 subjects: 10 healthy control subjects, 10 patients with potentially premalignant oral lesions with dysplastic changes and 10 others without, in addition to 10 patients suffering from OSCC. Levels of soluble CD44 (sCD44) were measured in whole unstimulated saliva (WUS) using an enzyme linked immuneassay (ELISA). In patients suffering from malignant lesions the salivary sCD44 level was correlating well with the grading of the lesion. Also, most of the patients with the highest salivary sCD44 levels showed postoperative relapse. A highly significant difference was found in the mean value of salivary sCD44 level between the control group and the premalignant with dysplasia and the cancer groups, and on the other hand, a non significant difference was found between the control and the premalignant without dysplasia group. Also, a highly significant difference was found between salivary sCD44 level in cancer patients and those with premalignant lesions without dysplasia, and non significant difference between the cancer patients and those with premalignant lesions with dysplasia. A ROC Curve was created to estimate salivary sCD44 level with the highest sensitivity and specificity which was 100% and 66.7% respectively. Results indicated that a level of salivary sCD44 lying within the range of 19.2 to 20.4 ng/ml could indicate malignant transformation within oral mucosal lesions.
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    Salivary microRNAs in oral cancer
    (Blackwell Publishing Ltd, 2015) Zahran F.; Ghalwash D.; Shaker O.; Al-Johani K.; Scully C.; Division of Oral Medicine; Oral Diagnostic Sciences Department; Faculty of Dentistry; King Abdulaziz University; Jeddah; Saudi Arabia; Department of Oral Medicine and Periodontology; Faculty of Oral and Dental Medicine; Cairo University; Cairo; Egypt; Department of Oral Medicine and Periodontology; Faculty of Dentistry; October University for Modern Sciences and Arts; 6th October City; Egypt; Department of Medical Biochemistry; Faculty of Medicine; Cairo University; Cairo; Egypt; UCL; London; United Kingdom
    Objective: This study investigated the use of three salivary microRNAs (miRNA-21, miRNA-184, and miRNA-145) as possible markers for malignant transformation in oral mucosal lesions. Materials and methods: Salivary whole unstimulated samples were collected from a study group of 100 subjects, consisting of 20 clinically healthy controls, 40 patients with oral potentially malignant disorders (PMDs) [20 with dysplastic lesions and 20 without dysplasia], 20 with biopsy-confirmed oral squamous cell carcinoma (OSCC), and 20 with recurrent aphthous stomatitis (RAS) as disease controls. Total RNA was isolated and purified from saliva samples using the microRNA Isolation Kit (Qiagen, UL). miRNA expression analysis was performed using qRT-PCR (Applied Biosystems). Results: There was a highly significant increase in salivary miRNA-21 and miRNA-184 in OSCC and PMD (with and without dysplasia) when compared to healthy and disease controls (P�<�0.001). Conversely, miRNA-145 levels showed a highly significant decrease in OSCC and PMD overall (P�<�0.001). RAS cases showed no significant difference from normal controls in any measured miRNA (P�>�0.05). The only microRNA to discriminate between OSCC and PMD with dysplasia was miRNA-184. When receiver operating characteristic curves were designed for the three miRNAs, cutoff points delineating the occurrence of malignant change were a fourfold increase in miRNA-21 with specificity 65% and sensitivity 65%, a 0.6 decrease in miRNA-145, with specificity 70% and sensitivity 60%, and a threefold increase of miRNA-184, with specificity 75% and sensitivity 80%. Calculating the area under the curve revealed that miRNA-184 was the only one among the studied miRNAs that provided good diagnostic value. Conclusion: Salivary determination of the miRNAs tested might furnish a noninvasive, rapid adjunctive aid for revealing malignant transformation in oral mucosal lesions, particularly miRNA-184. � 2015 John Wiley & Sons A/S.