Abstract:
Anti-phospholipid syndrome (APS) is an autoimmune disease characterized by thrombosis and
miscarriage events. Still, the molecular mechanisms underlying APS, which predisposes to a wide
spectrum of complications, are being explored. Seventy patients with primary and secondary APS
were recruited, in addition to 35 healthy subjects. Among APS groups, the gene expression levels
of XIST, Gab2, and TAK1 were higher along with declined miRNA155 level compared with controls.
Moreover, the sera levels of ICAM-1, VCAM-1, IL-1ꞵ, and TNF-α were highly elevated among APS
groups either primary or secondary compared with controls. The lncRNA XIST was directly correlated
with Gab2, TAK1, VCAM-1, ICAM-1, IL-1ꞵ, and TNF-α. The miRNA155 was inversely correlated with
XIST, Gab2, and TAK1. Moreover, ROC curve analyses subscribed the predictive power of the lncRNA
XIST and miRNA155, to diferentiate between primary and secondary APS from control subjects. The
lncRNA XIST and miRNA155 are the upstream regulators of the Gab2/TAK1 axis among APS patients
via infuencing the levels of VCAM-1, ICAM-1, IL1ꞵ, and TNF-α which propagates further infammatory
and immunological streams. Interestingly, the study addressed that XIST and miRNA155 may
be responsible for the thrombotic and miscarriage events associated with APS and provides new
noninvasive molecular biomarkers for diagnosing the disease and tracking its progression.
