Field-amplified sample stacking cyclodextrin modified capillary electrophoresis for quantitative determination of diastereomeric saponins
Emara S.; Masujima T.; Zarad W.; Mohamed K.; Kamal M.; Fouad M.; EL-Bagary R.
Date issued:
2014
Publisher:
Springer Netherlands
,
Oxford University Press
Series Info:
Journal of Chromatographic Science
52
Type:
Article
Keywords:
October University for Modern Sciences and Arts
,
جامعة أكتوبر للعلوم الحديثة والآداب
,
University of Modern Sciences and Arts
,
MSA University
,
beta cyclodextrin derivative
,
boric acid
,
plant extract
,
saponin
,
capillary electrophoresis
,
chemistry
,
clover
,
limit of detection
,
pH
,
plant seed
,
procedures
,
reproducibility
,
statistical model
,
beta-Cyclodextrins
,
Borates
,
Electrophoresis, Capillary
,
Hydrogen-Ion Concentration
,
Limit of Detection
,
Linear Models
,
Plant Extracts
,
Reproducibility of Results
,
Saponins
,
Seeds
,
Trifolium
Abstract:
Successful simultaneous diastereomeric separation and sensitive determination of two pairs of triterpenoidal saponins have been achieved by capillary electrophoresis (CE) using ?-cyclodextrin (?-CD) as a stereoselective agent to cooperate with borate complexation. A usual technique for isolation and group separation of saponins was developed as an appropriate purification step prior to the determination of individual saponins by CE. Soyasaponin I (S1), azukisaponin V (S2), bersimoside I (S3) and bersimoside II (S4) could be well separated within 14 min in a fused-silica capillary (60 cm long to the detector with an additional 10 cm to the cathode; 75 ?m i.d.). The background electrolyte was borate buffer (80 mM, pH 10), containing 24 mM ?-CD. The separation voltage was 14 kV with a detection wavelength of 195 nm. The sample was electrokinetically injected using a voltage of 16 kV for 12 s. Methanol (70%) was used as the diluent for field-amplified sample stacking after hydrodynamic injection of short water plug (5 cm, 4 s). The method was partially validated for linearity, repeatability, reproducibility, limits of detection and limits of quantification. The correlation coefficients of the calibration curves were all >0.998, and the recoveries were from 98.23 to 96.21%. The Author [2013]. Published by Oxford University Press. All rights reserved.
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