Seroprevalence of occult hepatitis B among Egyptian paediatric hepatitis C cancer patients

Raouf H.E.; Yassin A.S.; Megahed S.A.; Ashour M.S.; Mansour T.M.

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dc.contributor.author	Raouf H.E.
dc.contributor.author	Yassin A.S.
dc.contributor.author	Megahed S.A.
dc.contributor.author	Ashour M.S.
dc.contributor.author	Mansour T.M.
dc.contributor.other	Department of Microbiology and Immunology
dc.contributor.other	Faculty of Pharmacy
dc.contributor.other	Modern Sciences and Arts University
dc.contributor.other	Giza
dc.contributor.other	Egypt; Department of Microbiology and Immunology
dc.contributor.other	Faculty of Pharmacy
dc.contributor.other	Cairo University
dc.contributor.other	Kasr El-Aini St.
dc.contributor.other	Cairo
dc.contributor.other	11562
dc.contributor.other	Egypt; Cancer Biology Department
dc.contributor.other	National Cancer Institute
dc.contributor.other	Cairo University
dc.contributor.other	Cairo
dc.contributor.other	Egypt
dc.date.accessioned	2020-01-09T20:42:01Z
dc.date.available	2020-01-09T20:42:01Z
dc.date.issued	2015
dc.identifier.issn	13520504
dc.identifier.other	https://doi.org/10.1111/jvh.12260
dc.identifier.other	PubMed ID 24754376
dc.identifier.uri	https://t.ly/0Ewyl
dc.description	Scopus
dc.description.abstract	Occult hepatitis B infection is characterized by the presence of hepatitis B virus (HBV) DNA in the serum in the absence of hepatitis B surface antigen (HBsAg). Prevalence of hepatitis C virus (HCV) infections in Egypt is among the highest in the world. In this study, we aim at analysing the rates of occult HBV infections among HCV paediatric cancer patients in Egypt. The prevalence of occult HBV was assessed in two groups of paediatric cancer patients (HCV positive and HCV negative), in addition to a third group of paediatric noncancer patients, which was used as a general control. All groups were negative for HBsAg and positive for HCV antibody. HBV DNA was detected by nested PCR and real-time PCR. HCV was detected by real-time PCR. Sequencing was carried out in order to determine HBV genotypes to all HBV patients as well as to detect any mutation that might be responsible for the occult phenotype. Occult hepatitis B infection was observed in neither the non-HCV paediatric cancer patients nor the paediatric noncancer patients but was found in 31% of the HCV-positive paediatric cancer patients. All the detected HBV patients belonged to HBV genotype D, and mutations were found in the surface genome of HBV leading to occult HBV. Occult HBV infection seems to be relatively frequent in HCV-positive paediatric cancer patients, indicating that HBsAg negativity is not sufficient to completely exclude HBV infection. These findings emphasize the importance of considering occult HBV infection in HCV-positive paediatric cancer patients especially in endemic areas as Egypt. � 2014 John Wiley & Sons Ltd.	en_US
dc.description.uri	https://www.scimagojr.com/journalsearch.php?q=25300&tip=sid&clean=0
dc.language.iso	English	en_US
dc.publisher	Blackwell Publishing Ltd	en_US
dc.relation.ispartofseries	Journal of Viral Hepatitis
dc.relation.ispartofseries	22
dc.subject	October University for Modern Sciences and Arts
dc.subject	جامعة أكتوبر للعلوم الحديثة والآداب
dc.subject	University of Modern Sciences and Arts
dc.subject	MSA University
dc.subject	Cancer	en_US
dc.subject	Egypt	en_US
dc.subject	HBV	en_US
dc.subject	HCV	en_US
dc.subject	occult HBV	en_US
dc.subject	paediatric	en_US
dc.subject	alanine aminotransferase	en_US
dc.subject	alkaline phosphatase	en_US
dc.subject	aspartate aminotransferase	en_US
dc.subject	bilirubin	en_US
dc.subject	hepatitis B antibody	en_US
dc.subject	hepatitis B core antigen	en_US
dc.subject	hepatitis B surface antigen	en_US
dc.subject	hepatitis C antibody	en_US
dc.subject	virus DNA	en_US
dc.subject	adolescent	en_US
dc.subject	alanine aminotransferase blood level	en_US
dc.subject	alkaline phosphatase blood level	en_US
dc.subject	Article	en_US
dc.subject	aspartate aminotransferase blood level	en_US
dc.subject	bilirubin blood level	en_US
dc.subject	cancer patient	en_US
dc.subject	child	en_US
dc.subject	childhood disease	en_US
dc.subject	clinical article	en_US
dc.subject	controlled study	en_US
dc.subject	Egyptian	en_US
dc.subject	female	en_US
dc.subject	hepatitis B	en_US
dc.subject	Hepatitis B virus	en_US
dc.subject	Hepatitis B virus genotype D	en_US
dc.subject	hepatitis C	en_US
dc.subject	Hepatitis C virus	en_US
dc.subject	human	en_US
dc.subject	infant	en_US
dc.subject	male	en_US
dc.subject	occult hepatitis b	en_US
dc.subject	preschool child	en_US
dc.subject	prevalence	en_US
dc.subject	priority journal	en_US
dc.subject	real time polymerase chain reaction	en_US
dc.subject	school child	en_US
dc.subject	seroprevalence	en_US
dc.subject	virus detection	en_US
dc.subject	virus load	en_US
dc.subject	blood	en_US
dc.subject	chemistry	en_US
dc.subject	chronic hepatitis C	en_US
dc.subject	classification	en_US
dc.subject	complication	en_US
dc.subject	DNA sequence	en_US
dc.subject	Egypt	en_US
dc.subject	genetics	en_US
dc.subject	genotype	en_US
dc.subject	hepatitis B	en_US
dc.subject	isolation and purification	en_US
dc.subject	neoplasm	en_US
dc.subject	polymerase chain reaction	en_US
dc.subject	Adolescent	en_US
dc.subject	Child	en_US
dc.subject	Child, Preschool	en_US
dc.subject	DNA, Viral	en_US
dc.subject	Egypt	en_US
dc.subject	Female	en_US
dc.subject	Genotype	en_US
dc.subject	Hepatitis B	en_US
dc.subject	Hepatitis B Surface Antigens	en_US
dc.subject	Hepatitis B virus	en_US
dc.subject	Hepatitis C Antibodies	en_US
dc.subject	Hepatitis C, Chronic	en_US
dc.subject	Humans	en_US
dc.subject	Male	en_US
dc.subject	Neoplasms	en_US
dc.subject	Polymerase Chain Reaction	en_US
dc.subject	Real-Time Polymerase Chain Reaction	en_US
dc.subject	Sequence Analysis, DNA	en_US
dc.subject	Seroepidemiologic Studies	en_US
dc.title	Seroprevalence of occult hepatitis B among Egyptian paediatric hepatitis C cancer patients	en_US
dc.type	Article	en_US
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dcterms.source	Scopus
dc.identifier.doi	https://doi.org/10.1111/jvh.12260
dc.identifier.doi	PubMed ID 24754376
dc.Affiliation	October University for modern sciences and Arts (MSA)