A putative Chondroprotective role for IL-1β and MPO in herbal treatment of experimental osteoarthritis
Aborehab N.M.; El Bishbishy M.H.; Refaiy A.; Waly N.E.
Date issued:
2017
Publisher:
BioMed Central Ltd.
Series Info:
BMC Complementary and Alternative Medicine
17
Type:
Article
Keywords:
COMP
,
Curcumin
,
Ginger
,
HA
,
IL-1?
,
MDA
,
MPO
,
Osteoarthritis
,
alcohol
,
cartilage oligomeric matrix protein
,
curcumin
,
ginger extract
,
hyaluronic acid
,
interleukin 1beta
,
iodoacetic acid
,
malonaldehyde
,
myeloperoxidase
,
superoxide dismutase
,
cartilage oligomeric matrix protein
,
curcumin
,
hyaluronic acid
,
interleukin 1beta
,
malonaldehyde
,
peroxidase
,
plant extract
,
protective agent
,
superoxide dismutase
,
animal experiment
,
animal model
,
animal tissue
,
antiinflammatory activity
,
antioxidant activity
,
Article
,
chondroprotection
,
controlled study
,
drug effect
,
drug mechanism
,
Egypt
,
enzyme activity
,
enzyme linked immunosorbent assay
,
histopathology
,
liquid chromatography-mass spectrometry
,
male
,
nonhuman
,
osteoarthritis
,
protein blood level
,
randomized controlled trial
,
rat
,
animal
,
blood
,
chemistry
,
disease model
,
ginger
,
metabolism
,
Animals
,
Cartilage Oligomeric Matrix Protein
,
Curcumin
,
Disease Models, Animal
,
Ginger
,
Hyaluronic Acid
,
Interleukin-1beta
,
Male
,
Malondialdehyde
,
Osteoarthritis
,
Peroxidase
,
Plant Extracts
,
Protective Agents
,
Superoxide Dismutase
Abstract:
Background: Herbal treatment may have a chondroprotective and therapeutic effect on Osteoarthritis (OA). We investigated the mechanism of action of ginger and curcumin rhizomes cultivated in Egypt in treatment of OA in rat model. Methods: Thirty-five albino rats were intra-articularly injected with Monosodium Iodoacetate in the knee joint. Ginger and curcumin was orally administered at doses of 200 and 400mg/kg (F200 and F400). Serum levels of cartilage oligomeric matrix protein (COMP), hyaluronic acid (HA), malondialdehyde (MDA), myeloperoxidase (MPO), Interleukin-1 beta (IL-1?) and superoxide dismutase activity (SOD) were measured using ELISA. The composition of the herbal formula hydro-ethanolic extract was characterized using UPLC-ESI-MS. Histopathological changes in injected joints was examined using routine histopathology. Statistical analysis was performed using one-way ANOVA. Results: Serum levels of COMP, HA, MPO, MDA, and IL-1? were significantly decreased in F 200, F 400 and V groups when compared to OA group (P value <0.0001). On the other hand SOD levels were significantly elevated in treated groups compared to OA groups (P value <0.0001). Conclusions: The ginger/curcumin at 1:1 had chondroprotective effect via anti-inflammatory and antioxidant effect in rat OA model. Further pharmacological and clinical studies are needed to evaluate this effect. � 2017 The Author(s).
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