Investigating miRNA-661 and ATG4-B mRNA expression as potential biomarkers for hepatocellular carcinoma

Ali M.A.; Matboli M.; El-Khazragy N.; Saber O.; El-Nakeep S.; Abdelzaher H.M.; Shafei A.E.-S.; Mostafa R.

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Investigating miRNA-661 and ATG4-B mRNA expression as potential biomarkers for hepatocellular carcinoma

Ali M.A.; Matboli M.; El-Khazragy N.; Saber O.; El-Nakeep S.; Abdelzaher H.M.; Shafei A.E.-S.; Mostafa R.

Full Text link: https://t.ly/q2rjD

Date issued: 2018

Doi: https://doi.org/10.2217/bmm-2017-0273 PubMedID29441798

Publisher: Future Medicine Ltd.

Series Info: Biomarkers in Medicine
12

Type: Article

Keywords: ATG-4B , bioinformatics , biomarkers , hepatocellular carcinoma , miR-661 , prognosis , alanine aminotransferase , bilirubin , cysteine proteinase , messenger RNA , microRNA , microRNA 661 , protein ATG 4 , unclassified drug , ATG4B protein, human , autophagy related protein , cysteine proteinase , messenger RNA , microRNA , MIRN661 microRNA, human , tumor marker , adult , alanine aminotransferase blood level , area under the curve , Article , ATG4 B gene , bilirubin blood level , cancer prognosis , cancer staging , cancer survival , Child Pugh score , disease marker , female , gene , gene expression , human , liver cell carcinoma , liver cirrhosis , major clinical study , male , protein blood level , receiver operating characteristic , reverse transcription polymerase chain reaction , sensitivity and specificity , survival rate , blood , case control study , genetics , liver cell carcinoma , liver tumor , metabolism , middle aged , pathology , prognosis , upregulation , Area Under Curve , Autophagy-Related Proteins , Biomarkers, Tumor , Carcinoma, Hepatocellular , Case-Control Studies , Cysteine Endopeptidases , Female , Humans , Liver Neoplasms , Male , MicroRNAs , Middle Aged , Neoplasm Staging , Prognosis , RNA, Messenger , ROC Curve , Up-Regulation

Abstract:

Aim: We aimed to examine the statistical association between serum expression of miRNA 661 (miR-661) and ATG-4B mRNA and hepatocellular carcinoma (HCC) based on in silico data analysis followed by clinical validation. Patients & methods: Quantitative reverse-transcriptase real-time PCR was used to examine the expression of miR-661 and ATG-4B mRNA in the sera of HCC patients versus control. Results: The expression of miR-661 and ATG-4B mRNA was positive in 97.14 and 77.14%, respectively, in HCC patients. The survival analysis showed that ATG-4B mRNA was an independent prognostic factor. Conclusion: Our data are the first report of its kind regarding the considerable clinical significance of miR-661 and ATG-4B mRNA in HCC patients. � 2018 2018 Future Medicine Ltd.