Evaluation of osteopontin as a biomarker in Hepatocellular carcinomas in Egyptian patients with chronic HCV cirrhosis
Abdel-Hafiz S.M.; Hamdy H.E.; Khorshed F.M.; Aboushousha T.S.; Safwat G.; Saber M.A.; Seleem M.; Soliman A.H.
Date issued:
2018
Publisher:
Asian Pacific Organization for Cancer Prevention
Series Info:
Asian Pacific Journal of Cancer Prevention
19
Type:
Article
Keywords:
October University for Modern Sciences and Arts
,
University for Modern Sciences and Arts
,
MSA University
,
جامعة أكتوبر للعلوم الحديثة والآداب
,
Alpha-fetoprotein
,
Hepatocellular carcinoma
,
Osteopontin
,
Real-time PCR
,
alpha fetoprotein
,
osteopontin
,
SPP1 protein, human
,
tumor marker
,
adult
,
blood
,
case control study
,
chronic hepatitis C
,
complication
,
evaluation study
,
female
,
follow up
,
Hepacivirus
,
human
,
isolation and purification
,
liver
,
liver cell carcinoma
,
liver tumor
,
male
,
metabolism
,
middle aged
,
pathology
,
prognosis
,
receiver operating characteristic
,
virology
,
Adult
,
alpha-Fetoproteins
,
Biomarkers, Tumor
,
Carcinoma, Hepatocellular
,
Case-Control Studies
,
Female
,
Follow-Up Studies
,
Hepacivirus
,
Hepatitis C, Chronic
,
Humans
,
Liver
,
Liver Neoplasms
,
Male
,
Middle Aged
,
Osteopontin
,
Prognosis
,
ROC Curve
Abstract:
Background: Hepatocellular carcinoma (HCC) is a high incidence disease in Egypt with a poor prognosis and survival. Biomarkers are important for diagnosis of HCC at an early stage. Osteopontin (OPN), a glycoprotein secreted by macrophages, osteoblasts, and T cells, is also highly expressed in a variety of tumors, such as examples in the breast, colon, and stomach. The present study aimed to correlate the serum level of OPN in HCV-positive hepatocellular carcinoma patients, with OPN expression in tumor and non-tumor liver tissues in order to identify its efficacy as a biomarker for diagnosis. Material and Methods: Out of total of 146 patients, 80 were selected for inclusion in the study. Blood samples as well as specimens of tumor and non-tumor liver tissue were collected. In addition, blood samples from 20 healthy volunteers were obtained as controls. Serum OPN and alpha-fetoprotein (AFP) were evaluated by ELISA for HCC and control groups. OPN and AFP gene expression were examined by real-time PCR, after homogenization and DNA extraction from serum samples and liver tissues. Results: It was found that serum OPN levels were significantly higher in the HCC group compared to normal group (P=0.009), with a strong positive correlation with AFP expression. However, there was no significant difference between OPN expression in tumor and non-tumor liver tissue. Conclusion: Serum OPN is highly suggested to be a professional candidate for HCC early diagnosis, with a diagnostic ability and accuracy equal or higher than for AFP. � 2017, Asian Pacific Organization for Cancer Prevention.
Description:
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