In vivo anti-inflammatory activity and UPLC-MS/MS profiling of the peels and pulps of Cucumis melo var. cantalupensis and Cucumis melo var. reticulatus

Ezzat, Shahira M; Raslan M.; Salama M.M.; Menze E.T.; El Hawary S.S.

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dc.contributor.author	Ezzat, Shahira M
dc.contributor.author	Raslan M.
dc.contributor.author	Salama M.M.
dc.contributor.author	Menze E.T.
dc.contributor.author	El Hawary S.S.
dc.contributor.other	Department of Pharmacognosy
dc.contributor.other	Faculty of Pharmacy
dc.contributor.other	Cairo University
dc.contributor.other	Cairo
dc.contributor.other	11562
dc.contributor.other	Egypt; Department of Pharmacognosy
dc.contributor.other	Faculty of Pharmacy
dc.contributor.other	October University for Modern Science and Arts (MSA)
dc.contributor.other	6th October12566
dc.contributor.other	Egypt; Biotechnology and Life Sciences Department
dc.contributor.other	Faculty of Postgraduate Studies for Advanced Sciences
dc.contributor.other	Beni-Suef University62511
dc.contributor.other	Egypt; Department of Pharmacognosy
dc.contributor.other	Faculty of Pharmacy
dc.contributor.other	The British University in Egypt
dc.contributor.other	El-Sherouk City
dc.contributor.other	Egypt; Department of Pharmacology and Toxicology
dc.contributor.other	Faculty of Pharmacy
dc.contributor.other	Ain Shams University
dc.contributor.other	Cairo
dc.contributor.other	11566
dc.contributor.other	Egypt
dc.date.accessioned	2020-01-09T20:40:37Z
dc.date.available	2020-01-09T20:40:37Z
dc.date.issued	2019
dc.identifier.issn	3788741
dc.identifier.other	https://doi.org/10.1016/j.jep.2019.03.015
dc.identifier.other	PubMed ID 30857984
dc.identifier.uri	https://t.ly/py76r
dc.description	Scopus
dc.description	MSA Google Scholar
dc.description.abstract	Ethnopharmacological relevance: Cucumis melo var. cantalupensis and Cucumis melo var. reticulatus are the most famous varieties of netted muskmelon or cantaloupe in Egypt. Cantaloupe has a great reputation as an anti-inflammatory drug for hot inflammation of liver, cough, eczema, and kidney disorders such as ulcers in the urinary tract, and our objective was to confirm this use scientifically. Materials and methods: Inflammation was induced in adult male Sprague Dawley rats by subcutaneous injection of 0.05 ml of carrageenan (1% solution in saline) into the plantar surface of the right hind paw 30 min after oral pretreatment of the rats with 95% ethanolic extracts of Cucumis melo var. cantalupensis peels (CCP) and pulps (CCU) and Cucumis melo var. reticulatus peels (CRP) and pulps (CRU) at doses of 25 and 50 mg/kg. Indomethacin (10 mg/kg) was used as a standard drug. The effect of the tested samples was measured on the oedema volume, as well as PGE-2, TNF-? IL-6 and IL-1? levels. Metabolic profiling of the extracts was performed using UPLC-MS/MS analysis. Results: Pretreatment of rats with the ethanol extract of the pulps and peels of the two varieties at doses of 25 and 50 mg/kg significantly inhibited the carrageenan-induced increase in the oedema volume of the rat paws after 3 h, except for the low dose of the French cantaloupe pulp. CRP at 50 mg/kg caused the most significant reductions in both TNF-? (P < 0.05) and IL-1? (P < 0.001) levels, while CCP caused the most significant reductions in PGE-2 and IL-6 (P < 0.05) levels. Increases in PGE-2, TNF-? IL-6 and IL-1? levels were also significantly prevented by indomethacin (10 mg/kg). UPLC-MS/MS facilitated the identification of 44 phenolic compounds, including phenolic acids and flavonoids. Conclusion: This is the first report of the chemical and biological study of the peels of Cucumis melo var. cantalupensis and Cucumis melo var. reticulatus. � 2019	en_US
dc.description.uri	https://www.scimagojr.com/journalsearch.php?q=23015&tip=sid&clean=0
dc.language.iso	English	en_US
dc.publisher	Elsevier Ireland Ltd	en_US
dc.relation.ispartofseries	Journal of Ethnopharmacology
dc.relation.ispartofseries	237
dc.subject	October University for Modern Sciences and Arts
dc.subject	جامعة أكتوبر للعلوم الحديثة والآداب
dc.subject	University of Modern Sciences and Arts
dc.subject	MSA University
dc.subject	Cantaloupe	en_US
dc.subject	Interleukin-6	en_US
dc.subject	Peel	en_US
dc.subject	Tumour necrosis factor-?	en_US
dc.subject	UPLC-MS/MS	en_US
dc.subject	antiinflammatory agent	en_US
dc.subject	Cucumis melo var cantalupensis extract	en_US
dc.subject	Cucumis melo var reticulatus extract	en_US
dc.subject	flavonoid	en_US
dc.subject	indometacin	en_US
dc.subject	interleukin 1beta	en_US
dc.subject	interleukin 6	en_US
dc.subject	phenol derivative	en_US
dc.subject	plant extract	en_US
dc.subject	prostaglandin E2	en_US
dc.subject	tumor necrosis factor	en_US
dc.subject	unclassified drug	en_US
dc.subject	antiinflammatory agent	en_US
dc.subject	carrageenan	en_US
dc.subject	Il6 protein, rat	en_US
dc.subject	interleukin 1beta	en_US
dc.subject	interleukin 6	en_US
dc.subject	phytochemical	en_US
dc.subject	plant extract	en_US
dc.subject	prostaglandin E2	en_US
dc.subject	tumor necrosis factor	en_US
dc.subject	adult	en_US
dc.subject	animal experiment	en_US
dc.subject	animal model	en_US
dc.subject	antiinflammatory activity	en_US
dc.subject	Article	en_US
dc.subject	cantaloupe	en_US
dc.subject	carrageenan-induced paw edema	en_US
dc.subject	controlled study	en_US
dc.subject	Cucumis	en_US
dc.subject	Cucumis melo var reticulatus	en_US
dc.subject	dose response	en_US
dc.subject	fruit peel	en_US
dc.subject	fruit pulp	en_US
dc.subject	in vivo study	en_US
dc.subject	inflammation	en_US
dc.subject	liquid chromatography-mass spectrometry	en_US
dc.subject	male	en_US
dc.subject	metabolism	en_US
dc.subject	nonhuman	en_US
dc.subject	rat	en_US
dc.subject	animal	en_US
dc.subject	chemically induced	en_US
dc.subject	chemistry	en_US
dc.subject	edema	en_US
dc.subject	foot	en_US
dc.subject	fruit	en_US
dc.subject	liquid chromatography	en_US
dc.subject	muskmelon	en_US
dc.subject	pathology	en_US
dc.subject	phytotherapy	en_US
dc.subject	Sprague Dawley rat	en_US
dc.subject	tandem mass spectrometry	en_US
dc.subject	Animals	en_US
dc.subject	Anti-Inflammatory Agents	en_US
dc.subject	Carrageenan	en_US
dc.subject	Chromatography, Liquid	en_US
dc.subject	Cucumis melo	en_US
dc.subject	Dinoprostone	en_US
dc.subject	Edema	en_US
dc.subject	Foot	en_US
dc.subject	Fruit	en_US
dc.subject	Interleukin-1beta	en_US
dc.subject	Interleukin-6	en_US
dc.subject	Male	en_US
dc.subject	Phytochemicals	en_US
dc.subject	Phytotherapy	en_US
dc.subject	Plant Extracts	en_US
dc.subject	Rats, Sprague-Dawley	en_US
dc.subject	Tandem Mass Spectrometry	en_US
dc.subject	Tumor Necrosis Factor-alpha	en_US
dc.title	In vivo anti-inflammatory activity and UPLC-MS/MS profiling of the peels and pulps of Cucumis melo var. cantalupensis and Cucumis melo var. reticulatus	en_US
dc.type	Article	en_US
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dcterms.source	Scopus
dc.identifier.doi	https://doi.org/10.1016/j.jep.2019.03.015
dc.identifier.doi	PubMed ID 30857984
dc.Affiliation	October University for modern sciences and Arts (MSA)