Browsing by Author "Zaghloul S.S."

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Botanical and genetic characteristics of Lobularia libyca (viv). C.F.W. Meissn. (Brassicaceae)
(International Journal of Pharmacognosy and Phytochemical Research, 2014) Zaghloul S.S.; Nematallah K.A.; Al-Gendy A.A.; Ayoub N.; Pharmacognosy Department; October University for Modern Sciences and Arts (MSA); Giza; Egypt; Pharmacognosy Department; Zagazig University; Al-Sharkiya; Egypt; Pharmacognosy Department; Ain-Shams University; Cairo; Egypt; Pharmacognosy Department; BUE University; Cairo; Egypt
Lobularia libyca (viv). C.F.W. Meissn. is an annual herbaceous desert plant native to North Africa belonging to Family Brassicaceae (Cruciferae). Study of the botanical features was carried out for the root, the young and old stems, leaf, flower and seed of the plant. L. libyca was characterized by the presence of non-glandular branched unicellular two armed hair in the stem, leaf and flower while the root shows narrow cork and xylem occupies about 80% of the diameter of the root. The flower is characterized by hairy sepals and spherical pollen grains with three germ pores and three germ furrows. The pericarp is characterized by hairy epicarp and presence of fibrosclerides as innermost layer of the mesocarp. The septum shows pitted lignified parenchyma. The seed shows accumbent embryo and the testa is characterized by the presence of pigment layer with reddish brown content. The DNA of the plant was extracted from leaf samples and analyzed using eleven random decamer primers. A total of 89 random amplified polymorphic DNA (RAPD) markers were identified. Both the botanical study and the DNA fingerprint helped in the identification of the plant.
Chemical and biological investigation of essential oil of oroxylum indicum L. Leaves cultivated in Egypt
(International Journal of Pharmacognosy and Phytochemical Research, 2015) Zaghloul S.S.; Azzam S.M.; Eid H.H.; Hassan H.A.; Sleem A.A.; Pharmacognosy Department; Cairo University; Cairo; Egypt; Pharmacognosy Department; October University for Modern Sciences and Arts (MSA); Giza; Egypt; Pharmacology Department; National Research Center; Giza; Egypt
Hydrodistilled essential oil of fresh leaves of Oroxylum indicum L. family Bignoniaceae cultivated in Egypt was analyzed by gas chromatography?mass spectrometry (GC/MS) .The yield of essential oil was (0.2 %) . Thirty nine components were identified representing (89.85%). Ar-tumerone (19.37%), a sesquiterpene ketone, was the major constituent in the oil followed by methyl hexadecanoate (6.2%), laurenan-2-one (5.59%) and isopropyl butanoate (5.57%). The oxygenated constituents represents (63.81 %) while non- oxygenated constituents represents (26.04 %) .The oil showed significant cytotoxic activity on MCF7 (breast carcinoma cell line), HEPG2 (liver carcinoma cell line) and HELA (cervix carcinoma cell line) ,in addition to good in vivo and in vitro antioxidant activity. The oil also exhibited both hepatoprotective and heptocurative action, but showed weak antimicrobial activity against tested bacteria with no effect against fungi. � 2015, International Journal of Pharmacognosy and Phytochemical Research. All rights reserved.
Comparative study of volatile oil content and antimicrobial activity of pecan cultivars growing in Egypt
(2013) El Hawary S.S.; Zaghloul S.S.; El Halawany A.M.; El Bishbishy M.H.; Department of Pharmacognosy; Faculty of Pharmacy; Cairo University; Cairo 11562; Egypt; Department of Pharmacognosy; Faculty of Pharmacy; Modern Sciences and Arts University; Giza; Egypt; Faculty of Pharmacy; King Abdulaziz University; Jeddah; Saudi Arabia
The volatile oils obtained from the leaves of four pecan cultivars growing in Egypt were evaluated for their chemical composition and antimicrobial activity. The selected cultivars (cv.) were Carya illinoinensis (Wangneh.) K. Koch. cv. Wichita, C. illinoinensis cv. Western Schley, C. illinoinensis cv. Cherokee, and C. illinoinensis cv. Sioux. The gas chromatography-mass spectrometry analyses revealed that the volatile oils from samples of the different cultivars differ in composition and percentage of their components. ?-Curcumene was found as the major constituent of the cv. Wichita oil, whereas germacrene D was the major component of cv. Sioux, cv. Cherokee, and cv. Western Schley. The antimicrobial activity was assayed using the Kirby-Bauer Method by measuring the zone of inhibition of growth. All volatile oils displayed an antimicrobial activity against the tested bacterial strains. On the other hand, only the volatile oil of cv. Wichita showed an antifungal effect on Aspergillus flavus. This work has identified candidates of volatile oils for future in vivo studies to develop antibiotic substitutes for the diminution of human and animal pathogenic bacteria. Nevertheless, the variations of the volatile oil components and antimicrobial potencies of the different studied cultivars, necessitate identifying the cultivars used in future studies. � 2013 Mary Ann Liebert, Inc., and Korean Society of Food Science and Nutrition.
Glucosinolates profile, volatile constituents, antimicrobial, and cytotoxic activities of Lobularia libyca
(Institute of Electrical and Electronics Engineers Inc., 2016) Al-Gendy A.A.; Nematallah K.A.; Zaghloul S.S.; Ayoub N.A.; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA); Giza; Egypt; Faculty of Pharmacy; Zagazig University; Zagazig; Egypt; Faculty of Pharmacy; British University in Egypt; Cairo; Egypt; Faculty of Pharmacy; Ain-Shams University; Cairo; Egypt; Faculty of Medicine; Umm Al-Qura University; Makkah; Saudi Arabia
Context: Brassicaceae plants are associated with protection against cancers due to their glucosinolate contents. Objectives: We investigate fresh leaves, roots and ripe seeds of Lobularia libyca (Viv.) C.F.W. Meissn. (Brassicaceae) to identify their glucosinolate constituents, antimicrobial and cytotoxic activities Materials and methods: The glucosinolates were identified using GC-MS analysis of their hydrolysis products and LC-MS analysis in the case of seeds. Disc diffusion (1 mg/disc) and minimum inhibitory concentration (0�160 ?g/mL) methods were used to evaluate the antimicrobial activity of seed hydrolysate. In vitro cytotoxicity against colorectal HCT-116, hepatic HUH-7, breast MCF-7 and lung A-549 cells was evaluated for seed hydrolysate (0.01�100 ?g/mL) using the sulforhodamine B assay and doxorubicin as a standard Results: Three glucosinolates were identified for the first time in this plant and genus Lobularia. Glucoiberverin was the major compound accumulated in the seeds and leaves, while glucoiberin and glucoerucin were detected only in the seeds. No glucosinolates were detected in roots under the same experimental conditions. Other volatile constituents, e.g., terpenes and fatty acids were only identified in the seeds. The seed hydrolysate showed significant antimicrobial activities against Candida albicans and Pseudomonas aeruoginosa (MIC = 64 and 82 ?g/mL, respectively). The seed hydrolysate exhibited a marked selective cytotoxicity in vitro against colorectal, hepatic and breast cancer cell lines. The IC 50 values were 0.31, 2.25 and 37 ?g/mL, respectively. Discussion and conclusion: The results indicated the antimicrobial activity of L. libyca and the selective effect of the seed hydrolysate as a cytotoxic drug that is potentially more active than doxorubicin against HCT-116. � 2016 Informa UK Limited, trading as Taylor & Francis Group.
Polyphenols in Ammania auriculata: Structures, antioxidative activity and cytotoxicity
(Govi-Verlag Pharmazeutischer Verlag GmbH, 2014) Nawwar M.A.; Youb N.A.; El-Raey M.A.; Zaghloul S.S.; Hashem A.M.; Mostafa E.S.; Eldahshan O.; Werner V.; Becker A.; Haertel B.; Lindequist U.; Linscheid M.W.; Department of Phytochemistry and Plant Systematics; National Research Center; Dokki Cairo; Egypt; Department of Pharmacognosy; Faculty of Pharmacy; Ain-Shams University; Cairo; Egypt; October University for Modern Sciences and Arts; 6th October City; Egypt; Institute of Pharmacy; Pharmaceutical Biology; Ernst-Moritz-Arndt-Universit�t; Greifswald; Mecklenburg-Vorpommern; Germany; Department of Chemistry; Laboratory of Applied Analytical and Environmental Chemistry; Humboldt -University; Berlin; Germany
Chemical and biological investigations of the extract of Ammania auriculata (Lytheraceae) resulted in the identification of eight polyphenols (1 - 8) for the first time from this plant, including the gallotannin, 2,3,6-tri-O-galloyl-(?,?)-4C1-glucopyranose (8), for which 1D and 2D-NMR spectra were recorded and assigned for the first time. The structures of all isolates (1 - 8) were elucidated by conventional methods, spectroscopic analysis, including 1D and 2D NMR, and by HR-ESIMS as well. All of the isolated compounds were evaluated for their antioxidant activities, determined by the DPPH and ORAC methods and for their cytotoxicity against the keratinocyte cell line HaCaT using the neutral red assay (NRU) and cell cycle analysis. Compounds 1, 3, 4, 5, and 6 significantly inhibited reactive oxygen species production with ED50 values between 3.22 and 9.79 ?g/ml. Compounds 1, 3, 4, and 5 showed cytotoxic activity against HaCaT cells with IC50 values between 30.7 and 84.1 ?g/ml. The new galloyl glucose (8) was found not cytotoxic. Ellagitannins, 2,3-hexahydroxy-(?/?)-glucopyranose (1) and 1-O-galloyl 2,3-hexahydroxy-(?)-glucopyranose (5) possess remarkable antioxidative and comparably weak cytotoxic activity.
Profile of bioactive compounds in Nymphaea alba L. leaves growing in Egypt: Hepatoprotective, antioxidant and anti-inflammatory activity
(BioMed Central Ltd., 2017) Bakr R.O.; El-Naa M.M.; Zaghloul S.S.; Omar M.M.; October University for Modern Sciences and Arts (MSA); Pharmacognosy Department; Faculty of Pharmacy; Giza; Egypt; October University for Modern Sciences and Arts (MSA); Pharmacology Department; Faculty of Pharmacy; Giza; Egypt; October University for Modern Sciences and Arts (MSA); Pharmaceutics Department; Faculty of Pharmacy; Giza; Egypt; Universite Laval; Axe of Regenerative Medicine; Faculty of Medicine; Quebec City; Canada
Background: Nymphaea alba L. represents an interesting field of study. Flowers have antioxidant and hepatoprotective effects, rhizomes constituents showed cytotoxic activity against liver cell carcinoma, while several Nymphaea species have been reported for their hepatoprotective effects. Leaves of N. alba have not been studied before. Therefore, in this study, in-depth characterization of the leaf phytoconstituents as well as its antioxidant and hepatoprotective activities have been performed where N. alba leaf extract was evaluated as a possible therapeutic alternative in hepatic disorders. Methods: The aqueous ethanolic extract (AEE, 70%) was investigated for its polyphenolic content identified by high-resolution electrospray ionisation mass spectrometry (HRESI-MS/MS), while the petroleum ether fraction was saponified, and the lipid profile was analysed using gas liquid chromatography (GLC) analysis and compared with reference standards. The hepatoprotective activity of two doses of the extract (100 and 200 mg/kg; P.O.) for 5 days was evaluated against CCl4-induced hepatotoxicity in male Wistar albino rats, in comparison with silymarin. Liver function tests; aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), gamma glutamyl transpeptidase (GGT) and total bilirubin were performed. Oxidative stress parameters; malondialdehyde (MDA), reduced glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), total antioxidant capacity (TAC) as well as inflammatory mediator tumour necrosis factor (TNF)-? were detected in the liver homogenate. Histopathological examination of the liver and immunohistochemical staining of caspase-3 were performed Results: Fifty-three compounds were tentatively identified for the first time in N.alba leaf extract, where ellagitannins represent the main identified constituents. Nine hydrocarbons, two sterols and eleven fatty acids were identified in the petroleum ether extract where, palmitic acid and linolenic acids represented the major saturated and unsaturated fatty acid respectively. N.alba AEE significantly improved the liver function, oxidative stress parameters as well as TNF-? in addition to the amelioration of histopathological features of the liver and a profound decrease in caspase-3 expression. Conclusion: These results shed light on the hepatoprotective effect of N. alba that is comparable with that of silymarin. The antioxidant activities of N. alba extract in addition to the inhibition of crucial inflammatory mediator, as TNF-?, might be the possible hepatoprotective mechanisms. � 2017 The Author(s).