Browsing by Author "Younis S.A."

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    Novel mycosynthesis of cobalt oxide nanoparticles using Aspergillus brasiliensis ATCC 16404-optimization, characterization and antimicrobial activity.
    (Blackwell Publishing Ltd, 2020) Omran B.A.; Nassar H.N.; Younis S.A.; El-Salamony R.A.; Fatthallah N.A.; Hamdy A.; El-Shatoury E.H.; El-Gendy N.S.; Department of Processes Design & Development; Egyptian Petroleum Research Institute; Nasr City; Cairo; Egypt; Department of Microbiology; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA); 6th of October City; Egypt; Depratment of Analysis and Evaluation; Egyptian Petroleum Research Institute; Nasr City; Cairo; Egypt; Department of Civil and Environmental Engineering; Hanyang University; Seoul; South Korea; Department of Microbiology; Faculty of Science; Ain Shams University; Abbassia; Cairo; Egypt; Center of Excellence; October University for Modern Sciences and Arts (MSA); 6th of October City; Egypt
    Aims: Investigate the capability of Aspergillus brasiliensis ATCC 16404 to mycosynthesize Co3O4-NPs. Methods and Results: Mycelial cell-free filtrate of A. brasiliensis ATCC 16404 was applied for mycosynthesis of Co3O4-NPs. The preliminary indication for the formation of Co3O4-NPs was the change in colour from yellow to reddish-brown. One-factor-at a time-optimization technique was applied to determine the optimum physicochemical conditions required for the mycosynthesis of Co3O4-NPs and they were found to be: 72h for reaction time, pH 11, 30C, 100revmin?1 for shaking speed in the darkness using 4mmoll?1 of CoSO4.7H2O and 55% of A. brasiliensis dry weight mycelium (w/v). The mycosynthesized Co3O4-NPs were characterized using various techniques: spectroscopy including UV/Vis spectrophotometry, dynamic light scattering (DLS), zeta potential measurement, energy-dispersive X-ray analysis, Fourier transform infrared spectroscopy and X-ray diffraction; and vibrating sample magnetometry and microscopy including field emission scanning electron microscopy and high-resolution transmission electron microscopy. Spectroscopic techniques confirmed the formation of Co3O4-NPs and the microscopic ones confirmed the shape and size of the mycosynthesized Co3O4-NPs as quasi-spherical shaped, monodispersed nanoparticles with a nano size range of 20-27nm. The mycosynthesized Co3O4-NPs have excellent magnetic properties and exhibited a good antimicrobial activity against some pathogenic micro-organisms. Conclusion: Ferromagnetic Co3O4-NPs with considerable antimicrobial activity were for the first time mycosynthesized. Significance and Impact of the Study: The use of fungi as potential bionanofactories for mycosynthesis of nanoparticles is relatively a recent field of research with considerable prospects. 2019 The Society for Applied Microbiology