Browsing by Author "Waziry, Mahmoud Mostafa Fathy"

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    The Effect of Alterations in Water Quality Parameters on the Occurrence of Bacterial Diseases in Different Aquatic Environments
    (Nexus Academic Publishers, 01/11/2021) Khalefa, Hanan S; Abdel-Moneam, Dalia A; Ismael, Elshaimaa; Waziry, Mahmoud Mostafa Fathy; Ali, Mennaallah Samir Gaber; Zaki, Manal M
    The current study investigated the influence of adverse water quality parameters in different localities (Qarun Lake, Fayoum Province, Egypt; Mariotteya stream, Giza Governorate, Egypt; and Mediterranean coastal water, Alexandria Governorate, Egypt) on different fish species during the winter. Water and fish samples were collected to evaluate the physicochemical properties and heavy metal distribution in the water. Furthermore, bacteriological analysis of water and fish was performed. The physicochemical characteristics of water samples revealed high conductivity, alkalinity, and higher values of water hardness in Lake Qarun, Fayoum province and Mediterranean coastal water, Alexandria Governorate. However, the parameters in Mariotteya water were within normal limits. Heavy metals were detected in Lake Qarun and the Mediterranean Coast. However, none of the heavy metals analyzed were found in the Mariotteya water samples. Moreover, detected trace elements, cadmium and lead, exceeded the permissible limits. The bacterial load of the collected water samples revealed only the isolation of Escherichia coli and other coliform bacteria from Lake Qarun, while other bacteria included Vibrio spp. (2.48 log10 CFU/mL), Aeromonas spp. (2.70 log10 CFU/mL), and Pseudomonas spp. (3.18, 2.04 log10 CFU/mL) isolated from Lake Qarun and Mariotteya stream, and Staphylococcus spp. (2.00, 1.95, and 1.00 log10 CFU/mL) from Mariotteya stream, Lake Qarun, and Mediterranean Coast, respectively. Among the isolated bacteria from collected fishes, Aeromonas spp. weres detectable at a higher percentage (36.2%) followed by Vibrio spp. (31.4%), Pseudomonas spp. (16.2%), and Staphylococcus spp. (6.7%). Moreover, the highest percentage of bacterial isolates was recovered from Lake Qarun. Large shrimps from the Mediterranean Coast showed a high percentage of Vibrio spp. (40%) isolation. The log of viable microbial count and chemical parameters in water bodies had a strong correlation coefficient (r > 0.75), suggesting that the ecosystem is highly polluted by agricultural and industrial contamination © 2021 Khalefa et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
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    Efficiency of Chitosan/Graphene oxide Nanocomposite for Improvement of Water Quality
    (October University for Modern Sciences and Arts, 2020) Waziry, Mahmoud Mostafa Fathy
    The pollution of water became an international concern. Regardless which purpose water is used, it should not be contaminated by pathogenic microorganisms or harmful heavy metals. Chitosan nanoparticles (CNPs) have been considered potential antibacterial agents effective against a large variety of microorganisms. In addition to graphene oxide which plays a critical role in removal of heavy metals beside its antimicrobial activity. The biocompatible polymer mixed with the GO NPs led to low cytotoxicity on human cells, uniform size and excellent stability. This research mentions a critical estimation of the contribution of the mixed GO\CS nanomaterials in water treatment and analyze the physical, chemical and microbiological properties of surface water taken from several sites in Egypt (temperature, pH, turbidity, ammonia, Phosphorous. aeruginosa, Vibrio, and Flavobacterium) and thus improve these values using the nanocomposite. Water examination were done by chemical and heavy metal examination by ICP-OES elemental analysis technique and bacteriological examination of water and fish samples by a molecular identification of isolated bacteria through PCR and sequencing. GO\CS nanocomposite have been prepared by mixing the aqueous solution of chitosan and graphene oxide in the present of diluted acetic acid and the characterization of nanomaterial achieved by using TEM. The water examination was conducted and the result shows that samples were contaminated with Cu, Cd, and Zn. Graphene oxide and Chitosan nanocomposite was successfully prepared and added to the water sample which contains Staph.spp., Pseudomonous spp. And Aeromonas spp. And the heavy metals. The composite showed a positive result in the elimination and removal of water contaminant.