Browsing by Author "Wasfi, R"

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    Antimicrobial activity of Vitex agnus-castus Essential Oil and Molecular Docking Study of Its Major Constituents
    (2/26/2020) Bakr, R.O; Zaghloul, S.S; Hassan, R A; Sonousi, A; Wasfi, R; Fayed, M A A
    Antimicrobial resistance represents a public health problem worldwide that is associated with high morbidity and mortality which rose up the need for natural products as being an effective alternative. This study aims to evaluate the antimicrobial activity of the Vitex agnus-cactus L. essential oil (EO) towards bacterial and fungal strains of economic importance, besides, correlating its chemical constituents to the observed antimicrobial and antifungal activity using molecular docking. The chemical composition of essential oil was analyzed by gas chromatography-mass spectroscopy (GC-MS), where oxygenated monoterpenes (44.98 %) and monoterpenes (32.2 %) represented the major classes. Molecular docking study was carried out for the major identified essential oil constituents against bacterial protein targets, where, sabinene, 1,8 cineole, and linalool (the major oil constituents) acted on multi targets and reflected the effective antibacterial activity. Additionally, caryophyllene and verticiol showed a high binding affinity to Candida’s Farnesyl pyrophosphate synthase, a critical enzyme responsible for cell membrane integrity. V. agnus-cactus L. oil demonstrated itself as a powerful anticandidal agent providing a possible candidate in the pharmaceutical formulations. © 2020, © 2020 Har Krishan Bhalla & Sons. SciVal Topic Prominence
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    First-time characterization of viable but non-culturable Proteus mirabilis: Induction and resuscitation
    (Blackwell Publishing Inc., 2020) Ashour, H.M; Elshishtawy, H.M; Abdellatif, G.R; Wasfi, R
    Pathogenic bacteria can enter into a viable but non-culturable (VBNC) state under unfavourable conditions. Proteus mirabilis is responsible for dire clinical consequences including septicaemia, urinary tract infections and pneumonia, but is not a species previously known to enter VBNC state. We suggested that stress-induced P. mirabilis can enter a VBNC state in which it retains virulence. P. mirabilis isolates were incubated in extreme osmotic pressure, starvation, low temperature and low pH to induce a VBNC state. Resuscitation was induced by temperature upshift and inoculation in tryptone soy broth with Tween 20 and brain heart infusion broth. Cellular ultrastructure and gene expression were examined using transmission electron microscopy (TEM) and quantitative real-time polymerase chain reaction (qPCR), respectively. High osmotic pressure and low acidity caused rapid entry into VBNC state. Temperature upshift caused the highest percentage of resuscitation (93%) under different induction conditions. In the VBNC state, cells showed aberrant and dwarf morphology, virulence genes and stress response genes (envZ and rpoS) were expressed (levels varied depending on strain and inducing factors). This is the first-time characterization of VBNC P. mirabilis. The ability of P. mirabilis pathogenic strains to enter a stress-induced VBNC state can be a serious public health threat. © 2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.
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    Phenotypic detection of various β-lactamases among multidrug resistant isolates of Escherichia coli and Klebseilla pneumoniae isolated from urinary tract infections in some Egyptian hospitals
    (Egyptian Society of Biotechnology, 2014) Raslan, ARK; Wasfi, R; Mansy, MS; Ashour, MS
    In the present study a total of 205 isolates were recovered from186 different cases from urinary tract infections ward in some Egyptian hospitals (Dar El-Fouad Hospital, EL-Demerdash Hospital, El-Sheikh Zayed Hospital and Kasr Al-Ainy Hospital) from September 2011 to October 2012. The most predominant bacteria isolated from urine samples was Escherichia coli 49.76% (102/205), followed by Klebseilla pneumonia represented 14.63% (35/205). The growing number and rapid increase in antibiotic resistance among E. coli, Klebsiella spp. has prompted us to investigate the resistance mechanisms among these isolates. It was found that the rates of potential ESβL production among isolates of E. coli and Klebsiella spp. were 46.08% (47/102) and 60% (21/35) respectively. While, the rates of potential ESβLs and AmpC β-lactamase production among E. coli and Klebsiella spp., were 22.55% (23/102) and 25.71% (9/35), respectively. Our study revealed that the rates of carbapenemases production among E. coli and Klebsiella spp. were 0.98% (1/102) and 8.57% (3/35), respectively.