Browsing by Author "Shaker O.G."

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    Study of microRNAs-21/221 as potential breast cancer biomarkers in Egyptian women
    (Elsevier B.V., 2016) Motawi T.M.K.; Sadik N.A.H.; Shaker O.G.; El Masry M.R.; Mohareb F.; Biochemistry Department; Faculty of Pharmacy; Cairo University; Kasr El-Einy; Cairo; Egypt; Medical Biochemistry and Molecular Biology Department; Faculty of Medicine; Cairo University; Egypt; Biochemistry Department; Faculty of Dentistry; October University for Modern Sciences & Arts (MSA); Giza; Egypt; The Bioinformatics Group; School of Energy; Environment and AgriFood; Cranfield University; Bedford; MK43 0AL; United Kingdom
    microRNAs (miRNAs) play an important role in cancer prognosis. They are small molecules, approximately 17�25 nucleotides in length, and their high stability in human serum supports their use as novel diagnostic biomarkers of cancer and other pathological conditions. In this study, we analyzed the expression patterns of miR-21 and miR-221 in the serum from a total of 100 Egyptian female subjects with breast cancer, fibroadenoma, and healthy control subjects. Using microarray-based expression profiling followed by real-time polymerase chain reaction validation, we compared the levels of the two circulating miRNAs in the serum of patients with breast cancer (n=50), fibroadenoma (n=25), and healthy controls (n=25). The miRNA SNORD68 was chosen as the housekeeping endogenous control. We found that the serum levels of miR-21 and miR-221 were significantly overexpressed in breast cancer patients compared to normal controls and fibroadenoma patients. Receiver Operating Characteristic (ROC) curve analysis revealed that miR-21 has greater potential in discriminating between breast cancer patients and the control group, while miR-221 has greater potential in discriminating between breast cancer and fibroadenoma patients. Classification models using k-Nearest Neighbor (kNN), Nave Bayes (NB), and Random Forests (RF) were developed using expression levels of both miR-21 and miR-221. Best classification performance was achieved by NB Classification models, reaching 91% of correct classification. Furthermore, relative miR-221 expression was associated with histological tumor grades. Therefore, it may be concluded that both miR-21 and miR-221 can be used to differentiate between breast cancer patients and healthy controls, but that the diagnostic accuracy of serum miR-21 is superior to miR-221 for breast cancer prediction. miR-221 has more diagnostic power in discriminating between breast cancer and fibroadenoma patients. The overexpression of miR-221 has been associated with the breast cancer grade. We also demonstrated that the combined expression of miR-21 and miR-221can be successfully applied as breast cancer biomarkers. 2016 Elsevier B.V.
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    Visfatin -948 G/T and resistin -420 C/G polymorphisms in Egyptian type 2 diabetic patients with and without cardiovascular diseases
    (National Research Council of Canada, 2014) Motawi T.M.K.; Shaker O.G.; El-Sawalhi M.M.; Abdel-Nasser Z.M.; Biochemistry Department; Faculty of Pharmacy; Cairo University; Cairo; Egypt; Medical Biochemistry and Molecular Biology Department; Faculty of Medicine; Cairo University; Cairo; Egypt; Biochemistry Department; Faculty of Pharmacy; Modern Sciences and Arts University; Cairo; Egypt
    Diabetes mellitus is one of the main threats to human health in the 21st century. Visfatin/Nampt and resistin are novel adipokines that have been implicated in the pathogenesis of type 2 diabetes mellitus (T2DM) and cardiovascular disease (CVD) complication. Several genetic studies have shown inconsistent results regarding association of visfatin/Nampt gene (NAMPT) and resistin gene (RETN) polymorphisms with T2DM and CVD complications. Here, we investigate whether NAMPT -948G/T and RETN -420C/G polymorphisms are associated with T2DM, its CVD complications, and serum adipokines levels in 90 Egyptian diabetic patients (44 without CVD and 46 with CVD) along with 60 healthy control subjects. Higher frequencies of NAMPT -948G/G and RETN -420G/G were observed among T2DM patients compared with controls. Furthermore, the frequencies of these genotypes were significantly higher in T2DM patients with CVD than those without CVD. Both NAMPT -948G/G and RETN -420G/G genotypes and G alleles were significantly associated with T2DM and CVD in Egyptian diabetic patients. Moreover, serum visfatin/Nampt and resistin levels were markedly elevated in T2DM patients, with the highest values observed in G/G genotypes among T2DM patients with CVD. In addition, positive correlations were observed between plasma adipokines levels and CVD risk factors. In conclusion, our data suggests that genetic variations in NAMPT -948G/T and RETN -420C/G may contribute to the disposition for T2DM and its CVD complications in Egyptian patients. However, further studies with greater sample size should be performed to verify these results. 2014 Published by NRC Research Press.