Browsing by Author "Salman T.M."

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Adiponectin and E-selectin concentrations in relation to inflammation in obese type 2 diabetic patients with coronary heart disease(s)
(2011) El-Mesallamy H.O.; Hamdy N.M.; Salman T.M.; Mahmoud S.; Biochemistry Department; Faculty of Pharmacy; Ain Shams University; Cairo; Egypt; Biochemistry Department; Faculty of Pharmacy; AL-Azhar University; Cairo; Egypt; Modern Sciences and Arts University; Cairo; Egypt
Aim. Adipose tissue is now regarded as a source of proinflammatory mediators which may contribute to vascular injury, insulin resistance (IR), and atherogenesis, however, some of them have a protective role against vascular inflammation and/or IR; namely adiponectin and nitric oxide (NO). Adiponectin is a fat derived hormone, which enhances insulin sensitivity. In experimental studies adiponectin was shown to have anti-atherogenic properties by suppressing endothelial expression of adhesion molecules as endothelial-selectin (E-selectin) and inflammatory cytokines as high-sensitivity C-reactive protein (hsCRP), interleukin-1? (IL-1?), and monocyte chemotactic protein-1 (MCP-1). Therefore, the aim of the study was to evaluate plasma adiponectin, E-selectin, hsCRP, IL-1?, and MCP-1 concentrations in obese patients with and without coronary heart disease (CHD) having type 2 diabetes mellitus (DM) and evaluation of their relationship with selected anthropometric, biochemical, and clinical parameters. Methods. The study group consisted of (N.=70) males, 20 of which served as healthy non-obese controls (group I) (mean age 38.53.7 years; mean BMI 28 1.2 kg/m2). Patients enrolled in the study were classified into the following groups: type 2 DM obese subjects without CHD (group II) (N.=25) (mean age 42.23 years; mean BMI 32.11.4 kg/m2) and type 2 DM obese subjects with CHD (group III) (N.=25) (mean age 40.63 years; mean BMI 31.51.2 kg/m 2). Glucose and insulin estimation was performed and insulin resistance index (HOMA-IR) was calculated. In the fasting state, the plasma HbA1c, adiponectin, E-selectin, in comparison to hsCRP, IL-1?, MCP-1, and lipid parameters were estimated. Results. FBG, HbA 1c%, lipids, insulin, MDA, NO, hsCRP, IL-?, MCP-1, Adiponectin as well as E-selectin concentration were significantly different in patients with type 2 DM and CHD in comparison to patients without CHD and moreover, the healthy control group (P=0.01). There was a significant negative correlation between adiponectin and E-selectin (r=-0.642; P=0.0001). Conclusion. Our study supports the hypothesis that decreased level of adipokine(s), together with increased oxidative stress, pro-inflammatory marker(s) as well as endothelial adhesion molecule(s) contributes to the complex process of atherosclerosis in type 2 diabetic obese patients that may lead eventually to CHD.
Evaluating the role of curcum powder as a protective factor against bladder cancer - an experimental study
(Asian Pacific Organization for Cancer Prevention, 2012) El-Mesallamy H.; Salman T.M.; Ashmawey A.M.; Osama N.; Biochemistry Department; Faculty of Pharmacy; Egypt; Ain Shams University; National Cancer Institute; Cairo University; Egypt; Cancer Biology Department; National Cancer Institute; Cairo University; Cairo; Egypt; Al-Azhar University; National Cancer Institute; Cairo University; Egypt; MSA University; National Cancer Institute; Cairo University; Egypt
Throughout human history, plant products have been used for many purposes including as medicines. Herbal products and spices can be used as preventive agents against cancer due to their antimicrobial, antioxidant and antitumorigenic properties. This study was designed to evaluate the potential protective effect of curcum in rats administered nitrosamine precursors; dibutylamine (DBA) and sodium nitrate (NaNO3); and infected with Escherichia coli (E. coli) and also to monitor changes in nuclear factor the Kappa B p65 (NF-?B p56) pathway and its downstream products, Bcl-2 and interleukin-6 (IL-6), in parallel with nitrosamine precursors, E. coli and curcum treatment. Rats were divided into three groups (n=25 each; except of control group, n+20). Group I a normal control group, group II administered DBA/NaNO3 in drinking water and infected with E. coli and group III was administered DBA/NaNO3 in drinking water, infected with E. coli and receiving standard diet containing 1% curcum powder. Histopathological examination reflected that the curcum treated group featured a lower incidence of urinary bladder lesions,and lower levels of NF-?B, Bcl-2 and IL-6, than the group receiving nitrosamine precursor and infected with E. coli. These findings suggested that curcum may have a protective role during the process of bladder carcinogenesis by inhibiting the NF-?B pathway and its downstream products.
Role of chronic E. Coli infection in the process of bladder cancer- An experimental study
(2012) El-Mosalamy H.; Salman T.M.; Ashmawey A.M.; Osama N.; Biochemistry Department; Faculty of Pharmacy; Ain Shams University; Cairo; Egypt; Biochemistry Department; Faculty of Pharmacy; Al-Azhar University; Cairo; Egypt; Cancer Biology Department; National Cancer Institute; Cairo University; Cairo; Egypt; Biochemistry Department; Faculty of Pharmacy; Modern Sciences and Arts University; Cairo; Egypt
Background: Bladder cancer is a common malignancy in Egypt. A history of urinary tract infection can be considered as a risk factor for bladder cancer. Escherichia coli (E. coli) infection is responsible for 70% of urinary tract infection. This study aimed to evaluate the role of chronic E. coli infection during bladder carcinogenesis. In order to achieve this aim, we investigated the histopathological changes in bladder tissue and measured the level of nuclear factor kappa p65 (NF-Bp65), Bcl-2 and interleukin 6 (IL-6) in four groups each consisting of 25 male albino rats except of control group consisting of 20 rats. The first group was normal control group, the second group was infected with E. coli, the third group was administered nitrosamine precursor, and the forth group was infected with E. coli and administered nitrosamine precursor. Results: The histopathological examination revealed that E. coli infected group was able alone to produce some histopathological changes in bladder tissue and that nitrosamine precursor plus E. coli group showed highest incidences of urinary bladder lesions than the nitrosamine precursor group. NF-Bp65, Bcl-2 and IL-6 levels were significantly higher in nitrosamine precursor plus E. coli group than the other groups. Conclusion: These findings suggested that urinary bladder infection by E. coli may play a major additive and synergistic role during bladder carcinogenesis. � 2012 El-Mosalamy et al.; licensee BioMed Central.
Serum human leukocyte antigen-G and soluble interleukin 2 receptor levels in acute lymphoblastic leukemic pediatric patients
(Asian Pacific Organization for Cancer Prevention, 2012) Motawi T.M.K.; Zakhary N.I.; Salman T.M.; Tadros S.A.; Biochemistry Department; Faculty of Pharmacy; Egypt; Cancer Biology Department; National Cancer Institute; Egypt; Cairo University; Egypt; Al-Azhar University; Egypt; MSA University; Egypt
Aims and Background: Human leukocyte antigen-G and interleukin-2 receptor play pivotal roles in the proliferation of lymphocytes, and thus generation of immune responses. Their overexpression has been evidenced in different malignant hematopoietic diseases. This study aimed to validate serum soluble human leukocyte antigen-G (sHLA-G) and serum soluble interleukin-2 receptor (sIL-2R) as an additional tool for the diagnosis and follow up of acute lymphoblastic leukemia (ALL). Subjects and Methods: Both markers were determined by ELISA in the serum of 33 ALL pediatric patients before treatment and after intensification phase of chemotherapy as well as in the serum of 14 healthy donors that were selected as a control group. Results: ALL patients showed abnormal CBC and high serum lactate dehydrogenase, which were improved after chemotherapy. Also, there was a non-significant increase in serum sHLA-G in ALL patients compared with the control group. However, after chemotherapy, sHLA-G was increased significantly compared with before treatment. On the other hand, serum sIL-2R in ALL patients was increased significantly compared with the control group. After chemotherapy, sIL-2R decreased significantly compared with before treatment. Conclusions: From these results it could be suggested that measurement of serum sHLA-G might be helpful in diagnosis of ALL, while sIL-2R might be useful in diagnosis and follow-up of ALL in pediatric patients.