Browsing by Author "Sajid, Muhammad"

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    Metal-tolerant morganella morganii isolates can potentially mediate nickel stress tolerance in Arabidopsis by upregulating antioxidative enzyme activities
    (Landes Bioscience, 2024-03) Naqqash, Tahir; Aziz, Aeman; Baber, Muhammad; Shahid, Muhammad; Sajid, Muhammad; Emanuele, Radicetti; Gaafar, Abdel-Rhman Z; Hodhod, Mohamed S; Haider, Ghulam
    Plant growth-promoting rhizobacteria (PGPRs) have been utilized to immobilize heavy metals, limiting their translocation in metal contaminated settings. However, studies on the mechanisms and interactions that elucidate how PGPRs mediate Nickel (Ni) tolerance in plants are rare. Thus, in this study we investigated how two pre-characterized heavy metal tolerant isolates of Morganella morganii (ABT9 and ABT3) improve Ni stress tolerance in Arabidopsis while enhancing its growth and yield. Arabidopsis seedlings were grown for five weeks in control/Ni contaminated (control, 1.5 mM and 2.5 mM) potted soil, in the presence or absence of PGPRs. Plant growth characteristics, quantum yield, and antioxidative enzymatic activities were analyzed to assess the influence of PGPRs on plant physiology. Oxidative stress tolerance was quantified by measuring MDA accumulation in Arabidopsis plants. As expected, Ni stress substantially reduced plant growth (shoot and root fresh weight by 53.25% and 58.77%, dry weight by 49.80% and 57.41% and length by 47.16% and 64.63% over control), chlorophyll content and quantum yield (by 40.21% and 54.37% over control). It also increased MDA content by 84.28% at higher (2.5 mM) Ni concentrations. In contrast, inoculation with M. morganii led to significant improvements in leaf chlorophyll,­quantum­yield,­and­Arabidopsis­biomass­production.­The­mitigation­of­adverse­effects­of­Ni­stresson­biomass­observed­in­M. morganii-inoculated­plants­was­attributed­to­the­enhancement­of­antioxidative­ enzyme­ activities­ compared­ to­ Ni-treated­ plants.­ This­ upregulation­ of­ the­ antioxidative­ defensemechanism­ mitigated­ Ni-induced­ oxidative­stress,­ leading­to­ improved­ performance­ of­the­ photosynthetic­machinery,­which,­ in­turn,­enhanced­ chlorophyll­ content­ and­quantum­yield.­Understanding­theunderlying­mechanisms­of­these­tolerance-inducing­processes­will­help­to­complete­the­picture­of­PGP s-­ mediated­defense­signaling.­Thus,­it­suggests­that­M. morganii PGP s­candidate­can­potentially­be­utilizedfor­plant­growth­promotion­by­reducing­oxidative­stress­via­upregulating­antioxidant­defense­systems­inNi-contaminated­soils­and­reducing­Ni­metal­uptake.
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    New benzimidazole based Schiff bases as potent anti-alzheimer agents: Synthesis, bio-evaluation and molecular docking study
    (Elsevier, 2024-03) Othman, Mohamed s; Hayat, Shawkat; Rahim, Fazal; Taha, Muhammad; Sajid, Muhammad; Khan, Shoaib; Iqbal, Wajeeha; Shah, Syed Adnan Ali; Fareid, Mohamed A; Aboelnaga, Shimaa M; Abdel-Hafez, Lina JM; Hafez, Mohamed M
    In search of potent anti-Alzheimer agent benzimidazole based Schiff base derivatives (1–18) were synthesized and evaluated as dual inhibitor for acetylcholinesterase and butyrylcholinesterase enzymes. All analogs among the series except analog 1 and 16 showed a variable degree of inhibitory activity with IC50 value ranging between 0.10 ± 0.01 to 12.40 ± 0.30 µM for acetylcholinesterase and 0.20 ± 0.01 to 11.10 ± 0.30 µM for butyrylcholinesterase. The most potent analog found among the series was analog 8 having IC50 value 0.10 ± 0.01 and 0.20 ± 0.01 µM for both acetylcholinesterase and butyrylcholinesterase inhibition respectively. The structures of all synthesized analogs were confirmed through NMR and HR-EIMS. Structure activity relationship (SAR) has been established for all newly synthesized derivatives. To understand the binding interaction of most active derivatives with enzyme active site, molecular docking study were performed. The toxicity and mutagenicity of compound 8 was predicted using in silico software, namely Derek Nexus® (version 6.3). Various toxicity endpoints, including chromosomal damage, skin sensitization, hepatotoxicity were predicted. The degradation profile of compound 8 was predicted in silico by Zeneth software (version 9.0.1) resulting in a probability of the formation of seven potential degradation products.