Browsing by Author "Said M.M."

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A novel thermostable and halophilic thioredoxin reductase from the Red Sea Atlantis II hot brine pool
(Public Library of Science, 2019) Badiea E.A.; Sayed A.A.; Maged M.; Fouad W.M.; Said M.M.; Esmat A.Y.; Department of Biochemistry; Faculty of Science; Ain Shams University; Cairo; Egypt; Department of Biology; School of Sciences and Engineering; American University in Cairo; New Cairo; Egypt; Children Cancer Hospital; Cairo; Egypt; Faculty of Biotechnology; October University for Modern Sciences and Arts; 6th October City; Cairo; Egypt
The highly extreme conditions of the lower convective layer in the Atlantis II (ATII) Deep brine pool of the Red Sea make it an ideal environment for the search for novel enzymes that can function under extreme conditions. In the current study, we isolated a novel sequence of a thioredoxin reductase (TrxR) enzyme from the metagenomic dataset established from the microbial community that resides in the lower convective layer of Atlantis II. The gene was cloned, expressed and characterized for redox activity, halophilicity, and thermal stability. The isolated thioredoxin reductase (ATII-TrxR) was found to belong to the high-molecularweight class of thioredoxin reductases. A search for conserved domains revealed the presence of an extra domain (Crp) in the enzyme sequence. Characterization studies of ATIITrxR revealed that the enzyme was halophilic (maintained activity at 4 M NaCl), thermophilic (optimum temperature was 65�C) and thermostable (60% of its activity was retained at 70�C). Additionally, the enzyme utilized NADH in addition to NADPH as an electron donor. In conclusion, a novel thermostable and halophilic thioredoxin reductase has been isolated with a unique sequence that adapts to the harsh conditions of the brine pools making this protein a good candidate for biological research and industrial applications. � 2019 Badiea et al.
Synthesis and antitumor activity of some fused heterocyclic compounds based on cyclohepta[b]thiophene derivatives
(Bulgarian Academy of Sciences, 2014) El-Sharkawy K.A.; Said M.M.; Dardas G.; Department of Organic Chemistry; Faculty of Biotechnology; October University for Modern Sciences and Arts (MSA); El-Wahat Road; 6; October City; A. R.; Egypt; Pharmaceutical Chemistry Department; Pharmacy College; Jazan University; Jazan City; Saudi Arabia; Department of Organic Chemistry; Faculty of Pharmacy; Suez Canal University; Ismailia; A.R.; Egypt
The reaction of 5,6,7,8-tetrahydro-4H-cyclohepta[b]thiophene derivatives 3a, b with acetic anhydride in presence of glacial acetic acid produced the acetamido derivatives 4a, b. Cyclization of the latter compounds gave the annulated products 5a, b. Compounds 3a, b reacted with one of the activated methylene groups of malononitrile (2a) and afforded compounds 7a, b through internal cyclization of the intermediates of compounds 6a, b. The latter products were reacted with the cyclic ketones 8a, b, c in presence of elemental sulphur and afforded compounds 9a-f. Compounds 3a, b reacted with different types of aldehydes 10a, b, c to produce compounds 11a-f. Finally the products11a-f reacted with hydrazine hydrate (12) affording compounds 14a-f via the proposed intermediate formation of compounds 13a-f. The antitumor activities of the synthesized compounds were tested using three different cell lines. 2014 Bulgarian Academy of Sciences, Union of Chemists in Bulgaria.
Synthesis of novel S-acyl and S-alkylpyrimidinone derivatives as potential cytotoxic agents
(Springer Netherlands, 2016) Said M.M.; Taher A.T.; El-Nassan H.B.; El-Khouly E.A.; Pharmaceutical Organic Chemistry Department; Faculty of Pharmacy; Cairo University; 33 Kasr El-Aini Street; Cairo; 11562; Egypt; Organic chemistry Department; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA); Giza; Egypt
Abstract: Two series of 4-phenyl-5-cyanopyrimidin-6-one derivatives bearing various S-alkyl or S-acyl moieties at position 2 were prepared as cytotoxic agents. All compounds were tested for possible anti-cancer activity on two cell lines (MCF-7 and HCT-116). The MCF-7 cell line was found to be more sensitive than the HCT-116 cell line to the action of the compounds. Compound 8g was the most potent on the MCF-7 cell line with IC50 18.3�nM/mL, whereas its IC50 on the normal cell line (MRC-5) was 64.38�nM/mL, indicating its safety and selectivity towards the MCF-7 cell line. On the other hand, compound 8d was the most potent compound on the HCT-116 cell line with IC50 23.8�nM/mL. Compound 8g was screened against five kinases. The compound showed selective inhibitory activity against pim1 kinase with IC50 11.62��M. Graphical Abstract: [Figure not available: see fulltext.] � 2016, Springer Science+Business Media Dordrecht.